... attributed. Highlighted Article: The identification of 18 genes that regulate retinal ganglion cell regeneration reveals that, owing to a lack of concordance with widespread retinal injury paradigms, some genes function in a context-specific manner during regeneration. Ascl1 CRISPR screen...

... RNA-sequencing of nascent Otx2 + cells identified the bHLH factors Ascl1 and Neurog2 as candidate regulators. CRISPR/Cas9 targeting of these factors showed that only the simultaneous loss of Ascl1 and Neurog2 prevented OTX2 expression. Our findings suggest that Ascl1 and Neurog2 act either redundantly...

... the proneural gene achaete-scute homolog 1 ( Ascl1 ) contributes to VMH development. Ascl1 transcripts were detected in embryonic day (E) 10.5 to postnatal day 0 VMH neural progenitors. The elimination of Ascl1 reduced the number of VMH neurons at E12.5 and E15.5, particularly within the VMH-central (VMH C...

... are relevant to the difference in neurogenic potential between these two cell types, we tested them in dissociated cultures of MG using either mimics or antagomiRs to increase or reduce expression, respectively. Among the miRNAs tested, miR-25 and miR-124 overexpression, or let-7 antagonism, induced Ascl1...

... for later-acting zygotic signals. This pre-patterning drives the propensity of Xenopus animal cap cells to adopt neural fates under various experimental conditions. Previous studies found that the maternally expressed transcription factor, encoded by the Xenopus achaete scute-like gene ascl1 , is enriched...

...-helix transcription factors (TFs), Ascl1 and Ptf1a, have contrasting functions in specifying these neurons. To understand how Ascl1 and Ptf1a function in this process, we identified their direct transcriptional targets genome-wide in the embryonic mouse neural tube using ChIP-Seq and RNA-Seq. We show...

... in regeneration and repair. However, generation of physiologically mature neurons in vitro remains problematic. Here we demonstrate the cell-cycle-dependent phosphorylation of a key reprogramming transcription factor, Ascl1, on multiple serine-proline sites. This multisite phosphorylation is a crucial regulator...

... in a subset of p2 precursors constitutes the crucial first step towards generating neuronal diversity in this domain. The mechanism behind the asymmetric Notch activation and downstream signaling events are, however, unknown at present. We show here that the Ascl1 and Neurog basic helix-loop-helix (bHLH...

...Sini-Maaria Virolainen; Kaia Achim; Paula Peltopuro; Marjo Salminen; Juha Partanen Diverse mechanisms regulate development of GABAergic neurons in different regions of the central nervous system. We have addressed the roles of a proneural gene, Ascl1 , and a postmitotic selector gene, Gata2...

... ©2005. 2005 Spinal cord development Dorsal horn bHLH Neuronal specification Mouse Atoh1 Neurog2 Ascl1 The circuitry of the nervous system depends on complex connections of diverse neuronal cell types. Thus, defining the mechanisms that regulate neuronal diversity is fundamental...