... into sensory organs called neuromasts (NMs). Using single-cell RNA-sequencing and whole-mount in situ hybridization, we showed that mcf2lb is expressed in the pLLP during migration. Live imaging and subsequent 3D analysis of mcf2lb mutant pLLP cells showed disrupted apical constriction and subsequent rosette...

...-of-function experiments, we examine the roles of convergent extension and apical constriction, and define the role of the surface ectoderm during NTC. We show that NTC is a two-stage process with distinct spatiotemporal contributions of convergent extension and apical constriction at each stage. Convergent...

...Melisa A. Fuentes; Bing He ABSTRACT Epithelial folding mediated by apical constriction serves as a fundamental mechanism to convert flat epithelial sheets into multilayered structures. It remains unknown whether additional mechanical inputs are required for apical constriction-mediated folding...

...Ivan K. Popov; Heather J. Ray; Paul Skoglund; Ray Keller; Chenbei Chang ABSTRACT Apical constriction regulates epithelial morphogenesis during embryonic development, but how this process is controlled is not understood completely. Here, we identify a Rho guanine nucleotide exchange factor (GEF...

...Joel M. Brown; María J. García-García ABSTRACT Neural tube closure relies on the apical constriction of neuroepithelial cells. Research in frog and fly embryos has found links between the levels of intracellular calcium, actomyosin dynamics and apical constriction. However, genetic evidence...

... mouse liver development elucidates a novel self-organizing model of duct morphogenesis. NF2 Merlin ERM proteins Bile duct morphogenesis Lumen Apical constriction Mouse The coordinated establishment of polarity and communication among cells patterns epithelial tissues and can actively...

... neuroblasts as a model to study the apical constriction process during cell delamination. We observe dynamic myosin signals both around the cell adherens junctions and underneath the cell apical surface in the neuroectoderm. On the cell apical cortex, the nonjunctional myosin forms flows and pulses, which...

...Makoto Suzuki; Masanao Sato; Hiroshi Koyama; Yusuke Hara; Kentaro Hayashi; Naoko Yasue; Hiromi Imamura; Toshihiko Fujimori; Takeharu Nagai; Robert E. Campbell; Naoto Ueno Early in the development of the central nervous system, progenitor cells undergo a shape change, called apical constriction...

...Katsumi Fumoto; Hisako Takigawa-Imamura; Kenta Sumiyama; Tomoyuki Kaneiwa; Akira Kikuchi In lung development, the apically constricted columnar epithelium forms numerous buds during the pseudoglandular stage. Subsequently, these epithelial cells change shape into the flat or cuboidal pneumocytes...

... structure driven by an apical constriction, where apical accumulation of phospho-myosin light chain is positively regulated by FGF/ERK signaling. Our study delineates mechanisms governing the early formation of the MD, and also serves as a model of how an epithelial cell sheet is transformed to a tubular...

... apical junctions, with perturbations in apical constriction and actin accumulation. Similarly, knockdown of Fat1 in cortical precursors by in utero electroporation leads to overproliferation of radial glial precursors. Fat1 interacts genetically with the related cadherin Fat4 to regulate these processes...

...Richard A. Lang; Ken Herman; Albert B. Reynolds; Jeffrey D. Hildebrand; Timothy F. Plageman, Jr Apical constriction (AC) is a widely utilized mechanism of cell shape change whereby epithelial cells transform from a cylindrical to conical shape, which can facilitate morphogenetic movements during...

...Oliver A. Krupke; Robert D. Burke Apical constriction typically accompanies inward folding of an epithelial sheet. In recent years there has been progress in understanding mechanisms of apical constriction and their contribution to morphogenetic processes. Sea urchin embryos form a specialized...

...Daryl J. V. David; Qiming Wang; James J. Feng; Tony J. C. Harris Cell shape changes drive tissue morphogenesis during animal development. An important example is the apical cell constriction that initiates tissue internalisation. Apical constriction can occur through a phase of cyclic assembly...

... constriction and rosette assembly. We show that apical constriction in the LL primordium requires the activity of non-muscle myosin. We demonstrate further that shroom3 , a well-known regulator of non-muscle myosin activity, is expressed in the LL primordium and that its expression requires FGF signalling...

... The authors declare no competing financial interests. 25 6 2012 © 2012. Adherens junction remodelling Apical constriction EGFR signalling Bazooka Myosin II Drak Rok Drosophila During development, epithelial tissue remodelling requires regulated cell-cell...

... in the leading region. Fgf signaling was previously shown to be crucial for rosette formation in the pLLp. We demonstrate that activation of Fgf receptor (Fgfr) induces intracellular Ras-MAPK, which is required for apical constriction and rosette formation in the pLLp. Inhibiting Fgfr-Ras-MAPK leads to loss...

... eye when the lens placode forms the lens pit. This morphogenesis is accompanied by a columnar-to-conical cell shape change (apical constriction or AC) and is known to be dependent on the cytoskeletal protein Shroom3. Because Shroom3-induced AC can be Rock1/2 dependent, we hypothesized that during lens...

... J. D. , Harland R. M. , Wallingford J. B. ( 2003 ). Shroom induces apical constriction and is required for hingepoint formation during neural tube closure . Curr. Biol. 13 , 2125 - 2137 . Hashimoto H. , Ishikawa H. , Kusakabe M. ( 1999 ). Development...

.... apical constriction, basal nuclear migration) are poorly understood. We have identified a two-dimensional canonical BMP activity gradient in the chick neural plate that results in low and temporally pulsed BMP activity at the ventral midline/median hinge point (MHP). Using in vivo manipulations, high...