... not rescue Vg1-depleted embryos, we report that a second allele is effective. This work resolves the mystery of Vg1 function,and shows it to be an essential maternal regulator of embryonic patterning. Oligonucleotides (oligos) were as follows: Vg1A (antisense),5′-G * C * C * ATGTAACCTTG * A * G...

... and negative functions, dictates cell fate and behavior. Using an antisense approach to knockdown gene function in vivo, we find that each of the three Gli proteins is required for the induction of all primary neurons in the amphibian neural plate and regulates the bHLH/Notch neurogenic cascade. Analyses...

... alleles all contain transversions in highly conserved amino acids of the extracellular domains,suggesting these alleles produce dominant-negative effects. Antisense oligonucleotides that create specific splicing defects in the hab mRNA phenocopy the recessive phenotypes and, surprisingly, some of the ZMD...

... expression in the early embryo. * Author for correspondence (e-mail: heabq9@chmcc.org ) 5 8 2004 © 2004. 2004 Foxh1 Fast1 Antisense Nodal Xnr5 Xnr6 Xnr3 Xenopus FoxH1 (Fast1) was first characterized as the transcriptional partner for Smad proteins forming...