... meiotic progression, increased germ cell apoptosis, sloughing of germ cells and, eventually, infertility of mice. Mechanistically, we discovered that hnRNPH1 could interact with the splicing regulator PTBP1 in SCs to regulate the pre-mRNA alternative splicing of the target genes functionally related...

... Alternative splicing Uncoupling of transcription and translation Infertility Mouse National Key Research and Development Program of China http://dx.doi.org/10.13039/501100012166 2021YFC2700200 2018YFC1003500 Science, Technology, and Innovation Commission of Shenzhen Municipality...

... interactome, with depletion of mitochondrial proteins identified in the wild-type KIF2A interactome. Our data raises new insights about the importance of alternative splice variants during brain development. Fig. 5. Protein interactome of KIF2A isoforms is revealed by a proximity labeling proteomics...

...Luping Yu; Huiru Zhang; Xuebing Guan; Dongdong Qin; Jian Zhou; Xin Wu ABSTRACT Alternative splicing (AS) contributes to gene diversification, but the AS program during germline development remains largely undefined. Here, we interrupted pre-mRNA splicing events controlled by epithelial splicing...

... interneuron fates, findings that expand the known repertoire of terminal-selector actions. Finally, we reveal that the temporal diversification of PBX1 functions in neuronal specification is achieved, at least in part, through the dynamic regulation of alternative splicing. In Caenorhabditis elegans , PBX/CEH...

... is characterised by a specific set of alternatively spliced transcripts that code for proteins involved in the formation of the photoreceptor connecting cilium, pre-mRNA splicing and epigenetic modifiers. Importantly, our data show that the transition from foetal to adult retina is characterised by a large...

...-mediated mRNA decay Premature termination codon Alternative splicing Sertoli cell 3′UTR shortening Testis Gonocyte Spermatogenesis Sterility RNA-Seq Mouse Sertoli cells (SCs) are required not only for male-specific sexual differentiation during embryonic development, but also...

...Yasuno Iwasaki; Gerald H. Thomsen Alternative splicing of pre-mRNAs is an important means of regulating developmental processes, yet the molecular mechanisms governing alternative splicing in embryonic contexts are just beginning to emerge. Polyglutamine-binding protein 1 (PQBP1) is an RNA-splicing...

.... Central nervous system Drosophila ELAV/Hu Hox RNA-binding protein RNA processing Alternative polyadenylation (APA) Alternative splicing Segment-specific apoptosis The nervous system of vertebrates and invertebrates shows a remarkable level of regionalisation along the anteroposterior (AP...

...Luis F. de Navas; Hilary Reed; Michael Akam; Rosa Barrio; Claudio R. Alonso; Ernesto Sánchez-Herrero Although most metazoan genes undergo alternative splicing, the functional relevance of the majority of alternative splicing products is still unknown. Here we explore this problem in the Drosophila...

...Rahul N. Kanadia; Victoria E. Clark; Claudio Punzo; Jeffrey M. Trimarchi; Constance L. Cepko Alternative splicing is the primary mechanism by which a limited number of protein-coding genes can generate proteome diversity. We have investigated the role of the alternative-splicing factor Sfrs1...

...Qiuxia Guo; James Y. H. Li The vertebrate Fgf8 gene produces multiple protein isoforms by alternative splicing. Two evolutionarily conserved spliceforms, Fgf8a and Fgf8b, exhibit distinct bioactivities, with Fgf8b having a more potent inductive activity due to higher affinity for Fgf receptors...

... days E9.5 and E18.5. Embryos with complete lack of Wt1 and embryos with selective ablation of the alternatively spliced Wt1( + KTS) isoform both had thinner olfactory epithelia and fewer neuronal progenitor cells than do normal animals. Mash1 and neurogenin 1, two basic helix-loop-helix transcription...

... of multiple alternatively spliced sma-9 transcripts suggests protein isoforms with potentially different cell sublocalization and molecular functions. We propose that SMA-9 isoforms function as transcriptional cofactors that confer specific responses to DBL-1 pathway activation. To identify additional...

... isoform that provides a molecular explanation for the different phenotypic effects of lesions at this locus. Alternative splicing yields two EGL-15 proteins containing different forms of a domain located within the extracellular region of the receptors immediately after the first IG domain. Neither...

...Caroline A. Spike; Andrew G. Davies; Jocelyn E. Shaw; Robert K. Herman Previous work has shown that C. elegans MEC-8 is a putative RNA-binding protein that promotes specific alternative splices of unc-52 transcripts. unc-52 encodes homologs of mammalian perlecan that are located extracellularly...

... 06 1999 14 04 1999 © 1999 by Company of Biologists 1999 Sex-lethal Alternative splicing Drosophila Sex determination Protein:protein interaction Anti-Sxl monoclonals m114 and m5 are described in Bopp et al. (1991) . Anti-Sis-b antibody 5A10 is described in Deshpande...

.... The organization of the bobcat and Manx transcription units was determined by comparing genomic and cDNA clones. The Manx-bobcat gene locus has an unusual organization in which a non-coding first exon is alternatively spliced at the 5′ end of two different mRNAs. The bobcat and Manx genes are expressed...

... by alternative splicing of the first exon of the FHF- 2 gene and is predicted to encode a polypeptide with a distinct amino-terminus. Whole-mount in situ hybridization reveals restricted domains of expression of cFHF-1 and cFHF-2 in the developing neural tube, peripheral sensory ganglia and limb buds, and shows...

... is alternatively spliced to generate at least 7 secreted isoforms that differ only at their mature amino terminus, the biological significance of these multiple isoforms is not known. In this report, we demonstrate that multiple FGF-8 isoforms are present at sites of Fgf8 expression during mouse development...