... that the nonadherent cells were derived from the conceptus and not a maternal source, we isolated allantoises and chorions from conceptuses derived from crossing a transgenic β-actin/GFP male mouse with a wild-type female mouse. In both allantois and chorion cultures the nonadherent cells were GFP + , indicating...

... of the allantois and for amnion closure, as demonstrated by loss of Bmp signalling. By contrast, the appearance of ectopic PGC-like cells and regionalized ectopic vasculogenesis and haematopoiesis in thickened Smad5 m1/m1 amnion are amnion defects that have not been associated with loss of Bmp signalling...

... a plausible explanation for why T / T mutant cells were not found within some defective allantoises of otherwise chimeric T / T conceptuses( Wilson et al., 1993 ): instead of failure to be displaced from the streak into the allantois, which we showed was not the case ( Fig. 3A ), T / T cells likely...

... wild-type (D) and Cdx2 +/- /Cdx4 -/0 mutant allantois (E). The allantois of the wild type was slightly smaller than that of the compound mutant, explaining the lower Flk1 staining in D. ( F - I ) Pecam staining of allantoises isolated at E8.25 just after chorio-allantoic fusion. Primary endothelial...

... mesoderm to the allantois throughout the latter's early development, microsurgery was used to remove allantoises at ten developmental stages. Subsequent whole embryo culture of operated conceptuses resulted in the formation of regenerated allantoises at all time points. Aside from being generally shorter...

... chorioallantoic fusion and die by 10.5 days post coitus. The allantoises of Tbx4 -mutant embryos are stunted, apoptotic and display abnormal differentiation. Endothelial cells within mutant allantoises do not undergo vascular remodeling. Heterozygous embryos show a mild, transient growth defect in the allantois...

... in yolk sac or allantois; chorioallantoic fusion fails; the amnion does not expand with the growth of the embryo, but misexpresses vascular and hematopoietic markers. Separation of the bulk of yolk sac mesoderm from the endodermal layer and adherence between mesoderm of yolk sac and amnion, indicate...

... cells to the allantois. Finally, when removed from conceptuses and cultured in isolation, neural plate and headfold-stage allantoises formed a conspicuous vascular network that was positive for Flk-1. Hence, the vasculature of the allantois is formed intrinsically by vasculogenesis rather than...

... to the allantois. Scale Bars (A,C) 200 μm. (B) 100 μ Allantoises of lacZ /+ hemizygous genotype were removed through two vitelline sites ( Fig. 2 ): (1) through non-blood island tissue slightly distal to the level of the site of insertion of the allantois with the amnion, or (2) directly through visible...

... is the expression of Wnt2 in the early heart field of 7.5–8.5 dpc (days post-coitum) mouse embryos, making Wnt2 a potentially useful gene marker for the early stages of heart development. Expression was also detected in the allantois from 8.0 dpc and at later stages in the placenta and umbilicus. Mice deficient...

... experiments, transplanted allantoises typically attached to the chorion despite loss of their connection with the hindgut region of the fetus. Hence selective attachment of the allantois to the chorion clearly cannot depend simply on its directed growth. While the transplanted allantoic tissue attached...

... dpc T/T +/+ chimeras exhibit many morpho-logical features of intact T/T mutants. In addition, a dramatic bias of T/T cells towards caudal regions (such as tail and allantois) was observed in all chimeras tested. This is likely to result from accumulation of nascent T/T mesoderm cells with time near...

... allantois The int-1 family currently comprises only two members, int-1 and the int-1 -related protein (irp). int-1 was first identified as a gene frequently activated in mouse mammary tumor virus-induced mammary tumors due to insertion of viral sequences ( Nusse and Varmus, 1982 ; Nusse et...