...Anne Bourdais; Patricia Viard; Jenny Bormann; Côme Sesboüé; Daniel Guerrier; Nicole Therville; Julie Guillermet-Guibert; John Carroll; Guillaume Halet ABSTRACT The phosphoinositide 3-kinase (PI3K)/Akt pathway is thought to regulate key steps of mammalian oogenesis, such as dormant oocyte awakening...

... in craniofacial structures and other tissues containing neural crest (NC)-derived cells, we hypothesized that DDX3 is also important for NC development. Using Xenopus tropicalis as a model, we show that DDX3 is required for normal NC induction and craniofacial morphogenesis by regulating AKT kinase activity...

... is a secreted cystine-knot protein that functions in a positive feedback loop with FGF to activate Erk signaling and induce mesoderm. Wnt FGF Erk1 Akt Brachyury Nodal Antero-posterior axis Xenopus The vertebrate embryonic body plan forms via the specification of three germ layers...

... but significantly increased under differentiation conditions. Therefore, depletion of three PKD genes was able to phenocopy PKD inhibition. Mechanistically, PKD inhibition activated PI3K/AKT signaling by increasing the level of AKT phosphorylation, and the addition of a PI3K/AKT signaling pathway inhibitor...

... controls AKT signaling in gastrulas and glycolysis rate in adult cells. Here, we focus on PFKFB4 function in NC during and after neurulation, using time-controlled or hypomorph depletions in vivo . We find that PFKFB4 is essential both for specification of functional premigratory NC and for its migration...

...Jason S. L. Yu; Wei Cui Phosphatidylinositide 3 kinases (PI3Ks) and their downstream mediators AKT and mammalian target of rapamycin (mTOR) constitute the core components of the PI3K/AKT/mTOR signalling cascade, regulating cell proliferation, survival and metabolism. Although these functions...

...Wen Luo; Xia Zhao; Hengwei Jin; Lichan Tao; Jingai Zhu; Huijuan Wang; Brian A. Hemmings; Zhongzhou Yang Second heart field (SHF) progenitors exhibit continued proliferation and delayed differentiation, which are modulated by FGF4/8/10, BMP and canonical Wnt/β-catenin signaling. PTEN-Akt signaling...

... of activation of the serine/threonine kinase AKT in PGCs during EGC formation; notably, AKT activation, in combination with LIF and bFGF, enhanced EGC formation and caused ∼60% of E10.5 PGCs to become EGCs. The results indicate that the majority of PGCs at E10.5 could acquire pluripotency with an activated AKT...

... in the accelerated growth and temporal development resulting from re-oxygenation following hypoxia. We show that chronic hypoxia reduced, and re-oxygenation accelerated, embryonic growth and developmental rate. Whereas hypoxia repressed the Igf1 receptor and its downstream Erk1/2 and Akt signaling activities, re...

... to be a downstream target of Alk5 in vivo and in vitro. Loss of Alk5 -mediated signaling also stimulated Pten gene expression and inhibited ERK phosphorylation in vivo. Using lung epithelial cells, we show that Alk5 -regulated Hes1 expression is stimulated through Pten and the MEK/ERK and PI3K/AKT pathways. Thus...

... the molecular pathways active during epidermal barrier formation. Akt signaling increased as the barrier wave crossed epidermis and Jun was transiently dephosphorylated. Inhibitor experiments on embryonic explants showed that the dephosphorylation of Jun was dependent on both Akt and protein phosphatase 2A(Pp2a...

... analyzed the effect of the serine/threonine kinase AKT, one of the major downstream effectors of PI3K, on the developmental potency of PGCs. We used transgenic mice that expressed an AKT-MER fusion protein, the kinase activity of which could be regulated by the ligand of modified estrogen receptor (MER), 4...

... than previously thought. † Author for correspondence (e-mail: [email protected] ) * These authors contributed equally 14 9 2007 © 2007. 2007 TrkB TrkC BDNF Neurogenesis Gliogenesis Self-renewal Cortical precursors Neural stem cells In utero electroporation Akt...