...Lauren J. Walker; Rebecca A. Roque; Maria F. Navarro; Michael Granato ABSTRACT The receptor tyrosine kinase MuSK, its co-receptor Lrp4 and the Agrin ligand constitute a signaling pathway that is crucial in axial muscle for neuromuscular synapse development, yet whether this pathway functions...

... differentiation at developing neuromuscular junctions remains unknown. By using axon severing and microfluidic chamber assays, we first showed that treatment of a protein synthesis inhibitor, cycloheximide, inhibits agrin-induced presynaptic differentiation in cultured Xenopus spinal neurons. Newly synthesized...

... scanning electron microscopy combined with immunofluorescence analyses. We identified morphologically active EMT regions that associated with key components of the extracellular matrix, including the basement membrane-associated proteoglycan agrin. Deletion of agrin resulted in impaired EMT and compromised...

... matrix (ECM) components are present in the embryonic gut, but their role in regulating ENS development is largely unknown. Here, we identify heparan sulfate proteoglycan proteins, including collagen XVIII (Col18) and agrin, as important regulators of enteric neural crest-derived cell (ENCDC) development...

... role for LRP4 in dendrite and synapse formation during development. LRP4 Central nervous system development Synapse formation Dendritogenesis Transsynaptic tracing Agrin In utero electroporation PSD95 Bassoon Mouse Deutsche Forschungsgemeinschaft 10.13039/501100001659...

...Ruth Herbst; Ekaterina Avetisova; Steven J. Burden Mice lacking Musk, a muscle-specific receptor tyrosine kinase that is activated by agrin, fail to form neuromuscular synapses and consequently die at birth because of their failure to move or breathe. We produced mice that express a chimeric...

...James T. Campanelli; Gregory G. Gayer; Richard H. Scheller ABSTRACT Agrin is a component of the extracellular matrix that regulates aspects of neuromuscular junction differentiation. Identification of agrin-binding proteins has lead to the suggestion that α-dystroglycan is a muscle cell surface...