... as they colonized peripheral tissues during zebrafish development to determine whether cells tailor their migration strategy to their local tissue environment. We found that T cells in most sites migrated with F-actin-rich, leading-edge pseudopods, matching how they migrate in vitro . T cells notably deviated from...

... pole. Simultaneously, cortical microtubules disappear from the apical hemisphere but persist in the basal hemisphere. This is accompanied by the formation a dense network of fine actin filaments between the nucleus and the basal pole cortex. Experimental depolymerisation of either microtubules or actin...

...Briana Christophers; Shannon N. Leahy; David B. Soffar; Victoria E. von Saucken; Kendal Broadie; Mary K. Baylies ABSTRACT Cofilin, an actin-severing protein, plays key roles in muscle sarcomere addition and maintenance. Our previous work found that Drosophila cofilin ( DmCFL ) knockdown in muscle...

... results in defects of primary cilia, accompanied by increased RhoA activity and excessive actin polymerization at the basal body. We discovered that, mechanistically, Fuzzy interacts with and recruits the negative actin regulator ARHGAP35 (also known as p190A RhoGAP) to the basal body. We identified...

...Koichi Hasegawa; Takeshi K. Matsui; Junpei Kondo; Ken-ichiro Kuwako ABSTRACT During neural development, the actin filament network must be precisely regulated to form elaborate neurite structures. N-WASP tightly controls actin polymerization dynamics by activating an actin nucleator Arp2/3. However...

... the importance of the cytoskeleton, including actin filaments and microtubules, in rice development and environmental responses. Yet, reliable in vivo cytoskeleton markers are lacking in rice, which limits our knowledge of cytoskeletal functions in living cells. Therefore, we generated bright fluorescent marker...

...Meng-Meng Shan; Yuan-Jing Zou; Zhen-Nan Pan; Hao-Lin Zhang; Yi Xu; Jia-Qian Ju; Shao-Chen Sun ABSTRACT Mammalian oocyte maturation is a unique asymmetric division, which is mainly because of actin-based spindle migration to the cortex. In the present study, we report that a kinesin motor KIFC1...

... reconstruction Actin Development Image processing Nucleus Directional left-right (LR) asymmetry, which is evident in the external and internal morphology of many animals, is genetically determined ( Davison, 2020 ; Hobert et al., 2002 ; Inaki et al., 2018a , b ; Kuroda, 2015 ). Recent studies show...

... of network assembly, and provides insights into cell ratcheting, force transmission and pulsatility. Myosin Actin Morphogenesis Pulsatility Ratcheting Any observer who has watched an animal embryo develop has seen that the tissues that make up the embryo show a remarkable dynamism; indeed...

... crossover between sister PVD dendrites. Our genetic analysis suggests that FMI-1 promotes transient F-actin assembly at the tips of contacting sister dendrites to facilitate their efficient retraction during self-avoidance events, probably by interacting with WSP-1/N-WASP. Mutations of vang-1 , which...

... in corticofugal projections and axonal growth cones in Klc1 −/− mice. By live-cell imaging of CB1R-eGFP we characterized the axonal transport of CB1R vesicles and described the defects in transport that arise after KLC1 deletion. Cofilin activation, which is necessary for actin dynamics during growth cone...

... of a dynamic filopodium-like branchlet. The mechanisms underlying the initiation of dendrite branchlets are therefore crucial to the shaping of dendrites. Through in vivo time-lapse imaging of the subcellular localization of actin during the process of branching of Drosophila larva sensory neurons, combined...

...Ashley A. DeAguero; Lizzet Castillo; Sandy T. Oas; Kaveh Kiani; Anton L. Bryantsev; Richard M. Cripps ABSTRACT Serum response factor (SRF) has an established role in controlling actin homeostasis in mammalian cells, yet its role in non-vertebrate muscle development has remained enigmatic. Here, we...

...Hong-Hui Wang; Yu Zhang; Feng Tang; Meng-Hao Pan; Xiang Wan; Xiao-Han Li; Shao-Chen Sun ABSTRACT Cytoskeletal dynamics are involved in multiple cellular processes during oocyte meiosis, including spindle organization, actin-based spindle migration and polar body extrusion. Here, we report...

... of actomyosin activity, induces apical constriction, which is required for bottle cell formation during Xenopus gastrulation. Apical constriction Bottle cell Gastrulation RhoGEF Actin Myosin Xenopus National Institute of General Medical Sciences 10.13039/100000057 R01GM098566...

... and to specifically decipher the mechanisms controlling collective cell migration. Here, we identify the Drosophila Insulin/Insulin-like growth factor signalling (IIS) pathway as a key regulator in controlling actin dynamics in border cells, independently of its function in growth control. Loss of IIS activity blocks...

..., distributing in a PAR-dependent manner along the anterior–posterior (A–P) embryonic axis. PIP 2 cortical structures overlap with F-actin, and coincide with the actin regulators RHO-1 and CDC-42, as well as ECT-2. Particle image velocimetry analysis revealed that PIP 2 and F-actin cortical movements are coupled...

...Rob van der Kammen; Ji-Ying Song; Iris de Rink; Hans Janssen; Stefania Madonna; Claudia Scarponi; Cristina Albanesi; Wim Brugman; Metello Innocenti The Arp2/3 complex assembles branched actin filaments, which are key to many cellular processes, but its organismal roles remain poorly understood...

... 2017 http://www.biologists.com/user-licence-1-1/ Summary: This Review emphasizes the role of the actomyosin meshwork in determining the forces that operate within and between cells and their environment to shape and organize cells and tissues. Actin Contractility Morphogenesis...

... is associated with diseases including cancer. However, how the undesired activation of PKC is prevented during development remains poorly understood. We have previously shown that a protein kinase, IKKε, is active at the growing bristle tip and regulates actin bundle organization during Drosophila bristle...