... revolutionised 3D imaging of fluorescently labelled tissues, organs and embryos. However, the complexity and high cost of existing software and computing solutions limit their widespread adoption, especially by researchers with limited resources. Here, we present Acto3D, an open-source software, designed...

... much more accessible to non-specialists with the development of ready-to-use tools. The study of oogenesis mechanisms and female reproductive success has also recently benefited from the development of efficient protocols for three-dimensional (3D) imaging of ovaries. Such datasets have a great...

... of multi-gene families encoding ligands, receptors, pathway modulators and intracellular components. Here, we report an integrated analysis of key genes in the 3D space of the mouse embryo across multiple stages of development. We applied a method for 3D/3D image transformation to map all gene expression...

...-sequencing, 3D imaging and lineage tracing, we classify the mouse lung mesenchyme into three proximal–distal axes that are associated with the endothelium, epithelium and interstitium, respectively. From proximal to distal: the vascular axis includes vascular smooth muscle cells and pericytes that transition...

... be shown that the axon guidance defects are different in netrin 1 compared with DCC mutants, suggesting the presence of additional netrin 1 receptors ( Belle et al., 2014 ). 3D imaging is also extremely valuable in studying the interaction of embryos with their extra-embryonic tissues. For example...

... show parallels in the adjacent mesonephric ducts. Gonad Lightsheet Mesonephros Urogenital IDISCO Tissue clearing 3D imaging Morphogenesis * These authors contributed equally to this work Interactions between tissues are integral to the formation and morphogenesis...

... whole chicken embryos and embryo brains, improving imaging quality for confocal and light-sheet microscopy. Tissue clearing 3D imaging Chicken embryo Light-sheet microscopy The easy accessibility and physiological independence of chicken embryos have made them an important biological...

... sorted basal mammary epithelial cells (MECs). Analysis of Confetti-reporter mice revealed the formation of uni-colored structures and thus the clonal nature of these organoids. High-resolution 3D imaging demonstrated that basal cell-derived complex organoids comprised an inner compartment of polarized...

... of the generated mice will be embryonic lethal; therefore, phenotyping methods tuned to the mouse embryo are needed. Methods that are robust, quantitative, automated and high-throughput are attractive owing to the numbers of mice involved. Three-dimensional (3D) imaging is a useful method for characterizing...

... bundles, radiating from the nucleus, are involved in the earliest stages of cortical array formation. * Author for correspondence and reprints 14 08 1990 © 1990 by Company of Biologists 1990 microtubules interphase microtubule array 3D imaging confocal scanning laser...