...Sakthi Sundararajan; Maki Wakamiya; Richard R. Behringer; Jaime A. Rivera-Pérez The bacterial lacZ gene is widely used as a reporter in a myriad of mouse transgenic experiments. β-Galactosidase, encoded by lacZ , is usually detected using X-gal in combination with ferric and ferrous ions...

... to demonstrate that these Msx1-β-galactosidase positive cells are of somitic origin. We propose that these cells are dermal progenitor cells that migrate from the somites and subsequently contribute to the dorsalmost dermis. By analysing Msx1 nlacZ expression in a Splotch mutant, we observed that migration...

... sequences in the cytoplasmic domain with the E. coli β-galactosidase gene. The mutant Notch2 gene encodes a 380 kDa Notch2-β-gal fusion protein with β-galactosidase activity. Notch2 homozygous mutant mice die prior to embryonic day 11.5, whereas heterozygotes show no apparent abnormalities and are fully...

... forelimb (see Table 1 ), the whole bud expresses the Msx1 nlacZ gene (data not shown). We have generated mice heterozygous for both Msx1 nlacZ and Myf5 nlacZ . In such a double heterozygous embryo shown in Fig. 5a (35 somites), the β-galactosidase-positive cells of the progress zone...

... to purify PGCs early in development using vital stains for β -galactosidase such as fluorescein-di-galactoside (FDG) ( MacGregor et al., 1991 ). This will facilitate the isolation of new markers restricted to germ cells which could be of value to help understand those factors that govern allocation...

...Susanne Detterbeck; Piero Morandini; Birgit Wetterauer; Andreas Bachmair; Karin Fischer; Harry K. MacWilliams ABSTRACT In transgenic strains of Dictyostelium discoideum that express β-galactosidase under the control of a prespore-specific promoter, only early slugs show reporter confined...

... 1993 by Company of Biologists 1993 floor plate β-galactosidase histochemistry protein transfer axon guidance transgenic mice The floor plate is a column of non-neuronal cells that bridges the right and left halves of the neural tube at the ventral midline. Cells in this location...

... muscle regulatory factors and ubiquitous transcription factors . Mol. Cell. Biol . 11 , 267 – 280 MacGregor , G. R. , Mogg , A. E. , Burke , J. F. and Caskey , C. T. ( 1987 ). Histochemical staining of clonal mammalian cell lines expressing E. coli β galactosidase indicates...

... bearing a transgene in which the 1 kb DNA 5 regulatory sequence of the desmin gene is linked to a reporter gene coding for Escherichia coli β -galactosidase (Des1-nlacZ) . The enhancer activity of the desmin promoter is very strong and the reporter gene expression is easily detected in tissue sections. We...

... of Biologists 1991 homeobox genes LacZ β -galactosidase transgenic mice gene regulation Murine homeobox genes are expressed in stage-, region-, and tissue-specific patterns during embryonic development (for reviews, see Kessel and Gruss, 1990 ; Sashikant et al . 1990 ). The Hox-2 . 2...

... growth cycle. We examined this hypothesis by studying the expression of TIMP at selected times during a single hair cycle using T1MP– lacZ transgenic mice to localize TIMP gene activity in the hair follicle. TIMP gene induction was visualized by staining mouse back skin for β -galactosidase ( β -gal...

... -galactosidase activity was detected under these conditions when the same plasmid without a promoter was injected. Furthermore, β -galactosidase activity was restricted to those cells receiving the injected gene and their progeny; in 2 to 8 cell embryos where individual blastomeres were readily identified, only...

... of a β -galactosidase reporter gene. Numerous strains are stained in specific cells and at specific stages of oogenesis and provide useful ovarian markers for cell types that in some cases have not previously been recognized by morphological criteria. Since recent data have suggested that a substantial...

...Laurent Fasano; Stephen Kerridge ABSTRACT About 184 P[lac,ry + ]A insertions ( O’Kane & Gehring, 1987 ) have been incorporated into the genome via P element-mediated transformation. The temporal-spatial localization of β -galactosidase, synthesized by these insertions during oogenesis...