... of signaling pathways linked to native islet β-cell functional maturation, including evidence of fetal α-cell GLP-1 production and signaling to β-cells. Thus, the findings and resources detailed here show how pig pancreatic islet studies complement other systems for understanding the developmental programs...

... pancreas explants or in transgenic mice led to Pax6 downregulation and inhibition of α- and β-cell differentiation, resembling the molecular changes caused by haploinsufficient expression of Pax6. Accordingly, miR-7 knockdown resulted in Pax6 upregulation and promoted α- and β-cell differentiation...

... examine a decade of work on the contribution of vascular paracrine signals to developing tissues, with a focus on pancreatic β-cells. During the early stages of embryonic development, blood vessels are required for pancreas specification. Later, the vasculature constrains pancreas branching...

...David E. Metzger; Malgorzata Gasperowicz; Florian Otto; James C. Cross; Gerard Gradwohl; Kenneth S. Zaret Pancreatic β-cells arise from Ngn3 + endocrine progenitors within the trunk epithelium of the embryonic pancreas. The emergence of endocrine cells requires E-cadherin downregulation...

...M. Cristina Nostro; Farida Sarangi; Shinichiro Ogawa; Audrey Holtzinger; Barbara Corneo; Xueling Li; Suzanne J. Micallef; In-Hyun Park; Christina Basford; Michael B. Wheeler; George Q. Daley; Andrew G. Elefanty; Edouard G. Stanley; Gordon Keller The generation of insulin-producing β-cells from...

... reduction of β-cells, leading to some ambiguity as to the role of Hh during pancreas development and whether its function has completely diverged between species. Here, we have employed genetic and pharmacological manipulations to temporally delineate the role of Hh in zebrafish endocrine pancreas...

... pancreatic β-cell number, directing midline convergence ofβ-cells during early pancreatic islet formation, and specifying the anteroposterior location of foregut organs. Embryos deficient in cdx4 have a posteriorly shifted pancreas, liver and small intestine. The phenotype is more severe with knockdown...

... contributed equally to the work 22 12 2005 © 2006. 2006 Pancreas Zebrafish Insulin β-Cell Endoderm The vertebrate endoderm gives rise to the epithelial lining of the gut and to organs such as the thymus, liver and pancreas. This remarkable diversity of adult...

...Maike Sander; Lori Sussel; Jennifer Conners; David Scheel; Julie Kalamaras; Filemon Dela Cruz; Valerie Schwitzgebel; Andrea Hayes-Jordan; Michael German ABSTRACT Most insulin-producing β-cells in the fetal mouse pancreas arise during the secondary transition, a wave of differentiation starting...

... be expected from the mRNA pattern suggesting that fetal β -cells rapidly secrete insulin instead of storing it in the secretory granules. Alternatively, the translation of insulin mRNA could be inefficient. By double labeling the pancreas sections with polyclonal TGF- α antiserum and monoclonal insulin...