Issues

Summary: This Primer gives an overview of the essential roles of engrailed genes across species in establishing positional information during development, including the subdivision of territories into distinct compartments and the elaboration of specialized properties of subgroups of cells.

Summary: Culture of human embryonic stem cells on a micropattern system reveals that timely NODAL inhibition potentiates WNT signalling and directs anterior primitive streak progenitors to the notochord lineage.

Highlighted Article: The enhanced activity of phosphomutant ASCL1 depends on more efficient chromatin remodelling during in vitro neurogenesis.

Summary: Runx1 non-redundantly regulates a gene network in mouse satellite cells that is required for muscle maintenance and repair, which is mediated through interactions with Etv4 at ETS:RUNX composite motifs.

Summary: Genetic analyses in zebrafish reveal novel roles for cellular retinoic acid binding proteins in germ cells development and sex determination.

Highlighted Article: Single-cell RNA sequencing to track the reprogramming of missing mesoderm cells of sea urchin embryos reveals how each of three cell types is transfated beginning with early endoderm.

Highlighted Article: Muscle cell state is regulated by the imprinted Dlk1-Dio3 noncoding RNA locus through its coordinate control of mitochondrial activity and histone modifications.

Summary: GAS1 contributes to early kidney development through the integration of multiple different signaling pathways.

Summary: Castor acts in Type 2 neuroblast lineages as a temporal transcription factor to specify adult central complex columnar neuron identity.

Summary: Fgf20-expressing neuronal clusters act as short-range signalling centres that pattern proneural gene expression within hindbrain segments and induce glial fates in the segment centre.

Summary: The sclerotome, an embryonic structure within the somites, is the source of median fin cells and is reduced in smoothback mutant embryos, which do not form dorsal fins as adults.

Summary: SABER-seq is a CRISPR-Cas molecular recorder that stores transient developmental signaling inputs as permanent mutations in cellular genomes for extraction via single-cell transcriptomics.

Summary: A novel toolkit, utilising Tocky-canonical correspondence analysis, enables the identification of developmental trajectories in vivo through flow cytometric analysis using Fluorescent Timer proteins, elucidating temporal trajectories of thymic T-cell development.

Summary: A new resource that utilises MiMIC lines for endogenous gene tagging with MS2 and PP7 loops can be used for live imaging of transcription in a range of Drosophila tissues.