Issues

Summary: This Spotlight discusses recent studies using single-cell transcriptomics to investigate cellular heterogeneity within human inner ear organoids and their similarity to the native tissue.

Summary: This Review examines the role of bioengineered vasculature in tissue engineering and regenerative medicine, discussing design principles, self-assembly, microfluidics, 3D printing and challenges such as hierarchical branching, organ specificity and scalability for clinical use.

Summary: Human WNT7A is loaded on dendritic filopodia, triggering localised calcium signalling and synaptic protein clustering to form spines at specific dendrite locations.

Highlighted Article: When grafted into damaged pig sub-retinal space, polarized retinal sheets enriched in cone photoreceptor progenitors generated from human iPSCs survive and integrate the host retinal tissue, providing a framework for clinical tests.

Summary: Primary intestinal organoids rely on Lgr5⁺ intestinal stem cells for survival following genotoxic injury induced by doxorubicin treatment, clarifying the cell types necessary for intestinal organoid regeneration in vitro.

Summary: flt1 inactivation promotes zebrafish cardiac regeneration by enhancing coronary revascularization and endocardial expansion, while limiting myofibroblast differentiation.

Summary: Pax3-expressing cells generate neural crest and paraxial mesoderm. Here, we show that they also produce transient haematopoietic progenitors capable of generating embryonic myeloid and erythroid cells.

Highlighted Article: Delayed activation of the T-cell lineage commitment regulator Bcl11b in mouse thymic progenitors due to distal enhancer deletion reduces T-cell output and increases Zbtb16-driven ILC generation.

Summary: Control of ninjurin activity provides a common target through which diverse developmental, environmental and immune stimuli can be integrated to control haemocyte dispersal and immune function in Drosophila.

Summary: A cross-species study providing new understanding of the biochemical regulation by cAMP-PKA of oocyte meiotic resumption and how initiation of oocyte maturation has complexified during animal evolution.

Summary: ZNRF3-deficient embryonic male gonads exhibit pervasive disruption of sex-specific expression leading to unresolved gonadal supporting cell fates.

Summary: A previously unreported function for non-muscle myosin downstream of MRCK-1 in excretory canal outgrowth that may be conserved in the development of seamless tubes in other organisms.

Summary: The axonally synthesized m⁵C reader Ybx1 regulates axon growth by controlling local translation of its target mRNAs, which could be a general mechanism in both the central and peripheral nervous systems.

Highlighted Article: A previously unreported non-genomic androgen signaling pathway in embryonic development is revealed by a chemical-genetic screen in zebrafish.

Summary: The anatomy, symmetry and gene expression of the Marchantia polymorpha meristem changes during its lifespan, providing a foundational framework for identifying previously unreported mechanisms of meristem regulation.

Summary: Dysregulation of the CDK8 kinase module is linked to human diseases. This study reveals distinct roles of CDK8-CycC and Med12-Med13 in ribosome biogenesis, nascent protein synthesis, and cell-cycle progression.

Summary: Protein binding and genetic studies identify and characterize structural and functional motifs within the intracellular domain of Drosophila Dachsous.