Special Issue: Modelling Development in vitro
Summary: The combination of light-sheet fluorescence and expansion microscopy enables imaging of mature human brain organoids in toto and down to synaptic resolution.
Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions
Summary: An optimized protocol is presented that allows morphological, quantifiable differences between the damaged and control human neuronal networks to be detected in three-dimensional cultures.
Human neural progenitors establish a diffusion barrier in the endoplasmic reticulum membrane during cell division
Summary: Asymmetric segregation of cellular components, which may contribute to the fate and behavior of human neural progenitors, is allowed by a diffusion barrier in the endoplasmic reticulum membrane during cell division.
Summary: The key developmental transcription factor eomesodermin can replace Wnt priming in the differentiation of human embryonic stem cells into mesendoderm.
Human iPSC-derived cerebral organoids model features of Leigh syndrome and reveal abnormal corticogenesis
Summary: Induced pluripotent stem cell-derived model systems reveal defects in early neurogenesis due to dysregulation of key metabolic pathways associated with Leigh syndrome.
Generation of functional human oligodendrocytes from dermal fibroblasts by direct lineage conversion
Summary: This paper shows the generation of myelinating human oligodendrocytes from dermal fibroblasts using a direct lineage conversion technique, which could be of high interest for regenerative medicine applications.