Summary: This Primer gives an overview of how recent technological advances have improved our understanding of the multifaceted, context-dependent functions of SOX2 as a master regulator of stem cell activities.
Highlighted Article: Mouse preimplantation embryos are vulnerable to Zika virus-induced lethality even in the presence of the zona pellucida, highlighting a potential risk of sexually transmitted infection in early pregnancy.
Highlighted Article: Cell-autonomous growth of specialized cells, such as stomata, causes local growth variability that is buffered by their immediate neighbors to ensure reproducible organ development.
Gata2, Nkx2-2 and Skor2 form a transcription factor network regulating development of a midbrain GABAergic neuron subtype with characteristics of REM-sleep regulatory neurons
Highlighted Article: The identification of unique molecular markers of GABAergic neuron subtypes in the periaqueductal grey and midbrain reticular formation can facilitate studying the function of specific neuronal circuits.
Summary: Bmp7, known to be crucial for nephron progenitor cells, is also required throughout kidney development and has a role promoting growth of the postnatal kidney.
Single-cell transcriptomic signatures and gene regulatory networks modulated by Wls in mammalian midline facial formation and clefts
Summary: Single-cell genomic approaches reveal transcriptomic atlases of midfacial primordia, and a mesenchymal gene regulatory network modulated by Wls during midline facial formation and fusion.
Dynamic profiling and functional interpretation of histone lysine crotonylation and lactylation during neural development
Summary: Recently identified histone lysine acylations such as histone lysine crotonylation and lactylation are tightly correlated with gene expression and regulate neuronal fate during neural development via transcriptome remodeling.
AMPK activates the Nrf2-Keap1 pathway to govern dendrite pruning via the insulin pathway in Drosophila
Summary: Genetic analysis reveals that crosstalk between the AMPK-insulin pathway and the Nrf2-Keap1 pathway is involved in neuronal remodelling during Drosophila metamorphosis.
Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Highlighted Article: In early postnatal mice, rapid eye size increment is governed by a retinal pigment epithelium-derived signaling pathway, which is composed of both positive and negative regulators of eye growth.
Lrig1- and Wnt-dependent niches dictate segregation of resident immune cells and melanocytes in murine tail epidermis
Summary: Pigmentation and immune surveillance functions in murine tail skin are spatially segregated by Lrig1- and Wnt-Lef1-dependent keratinocyte lineages that control the partitioning of melanocytes and tissue-resident immune cells into distinct epidermal niches.
TECHNIQUES AND RESOURCES
Summary: Implemented as an easy-to-use Fiji plug-in, SAIBR provides effective autofluorescence correction for cells and tissues using standard off-the-shelf imaging conditions.
Highlighted Article: A new open-access Normal Table of Xenopus development is presented that integrates new embryo images with gene expression markers in Xenbase, the Xenopus biomedical research knowledgebase.