STEM CELLS AND REGENERATION
SyNPL: Synthetic Notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo
Summary: Optimised Synthetic Notch circuitry in mouse pluripotent stem cells provides a modular tool with which to monitor cell-cell interactions and program synthetic patterning of cell fates in culture and in embryos.
Summary: Genetic fate mapping and single-cell RNA sequencing reveal Procr expression as early as embryonic day 7.5 and demonstrate an essential role for Procr+ endothelial progenitor cells in establishment of the murine vasculature.
Hbxip is essential for murine embryogenesis and regulates embryonic stem cell differentiation through activating mTORC1
Summary: Characterization of the essential role of Hbxip in embryogenesis and embryonic stem cell differentiation, demonstrating that Hbxip is required for activating mTORC1 in these processes.
A non-disruptive and efficient knock-in approach allows fate tracing of resident osteoblast progenitors during repair of vertebral lesions in medaka
Summary: A novel CRISPR/Cas9 homology-directed knock-in approach allows fate tracing of resident collagen10a1 (col10a1) osteoblast progenitors during repair of vertebral lesions in a medaka osteoporosis model while preserving endogenous col10a1 expression.
Summary: Daw1 promotes the onset of timely cilia motility for robust axial straightening during zebrafish development.
Highlighted Article: Analyses of the relationships between chromatin architecture and regulatory activities at the HoxD locus show that ancestral transcription patterns can be maintained while new regulations evolve.
Nucleolin loss of function leads to aberrant Fibroblast Growth Factor signaling and craniofacial anomalies
Summary: Loss of function of the ribosomal RNA processing protein Nucleolin results in cartilage and bone hypoplasia in the craniofacial region of zebrafish, which can be rescued by FGF8 supplementation.
Highlighted Article: A genetic framework involving RPK2, CVP2, CVL1 and OPS reveals how Arabidopsis embryonic cotyledons modulate vascular branching for organ growth by an auxin-independent mechanism.
Feedforward regulatory logic controls the specification-to-differentiation transition and terminal cell fate during Caenorhabditis elegans endoderm development
Summary: This study describes the architecture of the gene regulatory network underlying endoderm development in C. elegans and illuminates the mechanisms that provoke specification, differentiation and commitment during embryogenesis.
The cellular function of ROP GTPase prenylation is important for multicellularity in the moss Physcomitrium patens
Summary: The unicellular state of the moss Physcomitrium patens is caused in part by uncontrolled cell expansion and defects in cell wall integrity, which are regulated by ROP GTPase activation by prenylation.
Summary: Analysis of Actrt1-knockout mice reveals that ACTRT1, ACTRT2, ACTL7A and ACTL9 form a complex of sperm-specific actin-related proteins, and ACTRT1 in the acroplaxome anchors developing acrosomes to the sperm nucleus.
The extra-embryonic area opaca plays a role in positioning the primitive streak of the early chick embryo
Summary: Two adjacent extra-embryonic tissues, the area opaca and the marginal zone, interact to influence the polarity of the early chick embryo.
Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice
Summary: Analysis of protamine 1-deficient mice revealed that heterozygous (Prm1+/−) males are subfertile, whereas homozygous males are infertile, with Prm1 deficiency leading to defective chromatin compaction and sperm damage.
Summary: Salt-triggered activation of pectin methyl esterase changes pectin in Arabidopsis, inducing at least two pathways: a CrRLK1L-dependent pathway downregulating salt stress responses and a CrRLK1L-independent pathway that activates downstream signaling.
Summary: Disruption of the biosynthesis of heparan sulfate and chondroitin sulfate enhances retinal permeability, suggesting their roles in the formation of the basement membrane and glycocalyx and maintenance of the retinal barrier.
Alignment of the cell long axis by unidirectional tension acts cooperatively with Wnt signalling to establish planar cell polarity
Highlighted Article: Unidirectional tissue tension deriving from morphogenetic movements aligns the cell long axis; this alignment acts cooperatively with Wnt signalling to establish planar cell polarity in Xenopus neuroectoderm.
Uncoupling transcription and translation through miRNA-dependent poly(A) length control in haploid male germ cells
Summary: miRNA-dependent poly(A) length control represents an important mechanism underlying uncoupled translation and transcription in male germ cells undergoing spermiogenesis.
TECHNIQUES AND RESOURCES
Fluorescent cytoskeletal markers reveal associations between the actin and microtubule cytoskeleton in rice cells
Summary: We generated rice cytoskeleton fluorescent marker lines and demonstrated cytoskeletal organization and associations in live rice cells, indicating the utility of the marker lines for investigations in rice biology.