Summary: The peri-implantation window of mammalian development is crucial for primordial germ cell (PGC) specification. This Review discusses the induction of PGCs in key mammalian models, including the new stem cell-based embryo models.
Dynamic extrinsic pacing of the HOX clock in human axial progenitors controls motor neuron subtype specification
Highlighted Article: Control by extrinsic factors of the sequential activation of HOX genes in axial progenitors is revealed by motor neuron subtype specification in human pluripotent stem cells.
Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives
Summary: An efficient strategy is described for the in vitro generation of previously hard-to-produce posterior spinal cord progenitors and MNs exhibiting a thoracic axial and, in particular, preganglionic columnar identity.
STEM CELLS AND REGENERATION
Ecdysone regulates the Drosophila imaginal disc epithelial barrier, determining the length of regeneration checkpoint delay
Summary: The Drosophila imaginal disc epithelial barrier matures in response to increasing ecdysone levels and re-localization of Cora along the lateral membrane: this limits Dilp8 signaling and determines the duration of regeneration-induced developmental delay.
PRDM16 regulates a temporal transcriptional program to promote progression of cortical neural progenitors
Summary: During neocortical development, PRDM16 promotes temporal progression of radial glia by regulating a dynamic gene expression program. This activity ensures the transition from mid- to late-neurogenesis.
Groucho co-repressor proteins regulate β cell development and proliferation by repressing Foxa1 in the developing mouse pancreas
Summary: Pancreatic β cells require Groucho co-repressors during development to repress a master regulator of the liver genetic program, Foxa1, and to allow Neurod1-mediated β cell expansion.
Highlighted Article: An in vitro study inferring that successful follicle assembly under the circulating physiological level of estrogen in the mouse fetus is achieved by liver-produced alpha-fetoprotein.
Summary: Actomyosin contractility guides the architectures of epithelial organs and regulates gene expression for progenitor cell patterning during branching morphogenesis.
Summary:abnormal pollen 1 encodes an L-type lectin receptor-like kinase and affects pollen starch accumulation through interaction with and phosphorylation of OsUGP2.
Highlighted Article: Evf2 lncRNA-enhancer targeting and long-range gene repression occur through regulation of pioneer transcription factor Sox2 in large subnuclear domains, controlling domain sizes, localization, site-specific Sox2 recruitment and DNA binding.
TECHNIQUES AND RESOURCES
A developmental lineage-based gene co-expression network for mouse pancreatic β-cells reveals a role for Zfp800 in pancreas development
Summary: Calculation of a gene co-expression network for pancreatic β-cell development reveals crucial roles for the clustered protocadherin genes and Zfp800 in pancreas development.
Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster
Summary: Single copies of short epitope tags were inserted into proteins of interest, allowing for in vivo binding and manipulation of the resulting chimeric proteins by genetically encoded epitope binders.