Summary: A novel hypothesis is presented incorporating a dynamic view of transcriptional states to explain how neural crest cells generate their diverse repertoire of cell types.
Highlighted Article: Given that KLF17 was shown to be sufficient, but not necessary, to establish naïve pluripotent hESCs, KLF17 might function as a peripheral regulator of human pluripotent stem cells.
STEM CELLS AND REGENERATION
Summary: This study reveals a new layer of regulation within the signaling network that controls differentiation of a Drosophila epithelial stem cell lineage.
Highlighted Article: Dynamic WNT11 expression in the cecal region regulates enteric neural crest cell colonization of the colorectum, revealing an essential role of the ceca in avian enteric nervous system development.
Summary: Epigenetic machinery centered on a LncRNA and the BAF complex temporally controls cell cycle progression and the neurogenic program of neocortical intermediate progenitors.
Live imaging of retinotectal mapping reveals topographic map dynamics and a previously undescribed role for Contactin 2 in map sharpening
Summary: This study describes the first visualization of a topographic map forming and maturing in a living animal and identifies the adhesion molecule Contactin 2 as a key regulator in that process.
TECHNIQUES AND RESOURCES
Hybridization chain reaction enables a unified approach to multiplexed, quantitative, high-resolution immunohistochemistry and in situ hybridization
Summary: Signal amplification based on the mechanism of hybridization chain reaction enables multiplexed, quantitative, high-resolution imaging of protein and RNA targets in highly autofluorescent tissues.
Summary: Use of proximity biotin ligation to identify components of a Dynein localization complex and the development of a nanobody-based approach to identify the interactome of any GFP-tagged protein.
Single-cell reconstruction with spatial context of migrating neural crest cells and their microenvironments during vertebrate head and neck formation
Summary: A comprehensive map of the transcriptional landscape of the first four chick branchial arches that defines a spatio-temporal reference resource during patterning of the vertebrate head and neck.