Summary: This Primer provides an overview of the role of TEAD proteins during development and homeostasis, highlighting how TEADs interact with different co-factors to drive distinct transcriptional and morphological outcomes.
The road to generating transplantable organs: from blastocyst complementation to interspecies chimeras
Summary: This Review summarizes the progress made in and roadblocks hindering the growth of human organs in animals via interspecies blastocyst complementation approaches.
STEM CELLS AND REGENERATION
Summary: An organoid model of neural tube extension can be used to dissect the cellular organization and patterning decisions that dictate early human nervous system development.
HoxD transcription factors define monosynaptic sensory-motor specificity in the developing spinal cord
Summary: Members of the HoxD family of transcription factors play an integral role in the synaptic specificity of monosynaptic sensory-motor connections in the developing spinal cord.
Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E
Summary: Combined use of a PB [Act-RFP] transgenesis model, selective pharmacological inhibition and expression analyses verified the essential role of eIF4E in the transition from maternal to embryonic control of mouse development.
Summary: PHF8 demethylates astrogenic and synaptogenic genes during astrocyte differentiation, and astrocytic PHF8 depletion has a striking effect on neuronal synapse formation and maturation in vitro.
EHF is essential for epidermal and colonic epithelial homeostasis, and suppresses Apc-initiated colonic tumorigenesis
Highlighted Article: A novel whole-body knockout mouse lacking the Ets DNA-binding domain of EHF, reveals an essential role for the EHF transcription factor in the maintenance of epidermal and intestinal epithelial cell homeostasis, and that its loss promotes colon tumour development.
Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 activity
Summary: An enhancer and the transcription factors Ascl1 and Neurog2 are required for retinal Otx2 expression. The Otx2 regulatory network is surprisingly flexible during retinal development.
Laminin alpha 5 regulates mammary gland remodeling through luminal cell differentiation and Wnt4-mediated epithelial crosstalk
Summary: Luminal mammary epithelial cells produce basement membrane laminin α5 necessary for mammary epithelial growth and differentiation. Laminin α5 loss compromises hormone receptor-positive luminal cell function and Wnt4-mediated crosstalk between epithelial cells.
The complete cell lineage and MAPK- and Otx-dependent specification of the dopaminergic cells in the Ciona brain
Summary: A detailed analysis of the complete cell lineage of Ciona larval dopaminergic cells reveals the mechanisms and regulatory factors involved in commitment to the dopaminergic cell fate.
TECHNIQUES AND RESOURCES
Summary: A reverse genetic system to identify essential regulators of reproductive and maternal-effect processes is used to disrupt genes with known maternal-effect functions.