Summary: This Meeting Review summarizes the main results presented at the EMBL/IBEC Winter conference ‘Engineering Multicellular Systems’ held in Spain in February 2020.
Summary: This Review discusses how neuronal and glial development affects CNS angiogenesis and barriergenesis, and how this might lead to new interventions to treat neurovascular pathologies.
STEM CELLS AND REGENERATION
Survival of Drosophila germline stem cells requires the chromatin-binding protein Barrier-to-autointegration factor
Summary: Emerin/Otefin, a Drosophila nuclear lamina LEM-D protein, plays a major role in localizing Barrier-to-autointegration factor to the nuclear lamina, a partnership that contributes to stem cell maintenance.
Highlighted Article: Aged muscle stem cells display delayed activation dynamics, but retain a youthful activation trajectory, suggesting that changes to cell state dynamics might contribute to aging pathology.
ETHYLENE INSENSITIVE 3 suppresses plant de novo root regeneration from leaf explants and mediates age-regulated regeneration decline
Summary: Enhanced EIN3 activity in older plants suppresses WOX11/WOX5 activation to inhibit de novo root regeneration in detached Arabidopsis leaf explants.
WNT regulates programmed muscle remodeling through PLC-β and calcineurin in Caenorhabditis elegans males
Summary: Fluorescently tagged actin visualization, mutant analyses and Ca2+-based assays reveal the mechanisms that underpin muscle remodeling under a normal developmental program, which provides insights into how to control pathological remodeling.
A combinatorial code of transcription factors specifies subtypes of visual motion-sensing neurons in Drosophila
Summary: Morphological and transcriptomic analyses allowed the identification of a combinatorial code of transcription factors that controls the development of subtype-specific morphologies in motion-detecting neurons of the Drosophila visual system.
Highlighted Article: Analysis of conditional and temporal knockout mice reveals roles of Ebf3 in the differentiation of lateral plate mesenchymal cells towards pre-osteoblasts during sternum ossification at the boundary of the lateral and somitic mesoderm.
Highlighted Article: Embryonic Shh signalling integrates flight feather with digit specification in the chicken wing, providing insights into the evolution of avian flight.
TECHNIQUES AND RESOURCES
Highlighted Article: We identify endogenous CRISPR/Cas9 arrays from wild-type genomes, making whole-organism lineage tracing more feasible in non-model species for which it is hard to generate transgenic animals.
MicroRNA profiling of mouse cortical progenitors and neurons reveals miR-486-5p as a regulator of neurogenesis
Summary: Use of a double reporter mouse line reveals miRNA profiles of progenitor cell types (proliferating and differentiating) and newborn neurons during mouse cortical development.