Issues

Summary: This Spotlight article describes the major impact and influence Suzanne Eaton's work has had, and still has, on planar cell polarity-associated studies.

Summary: This Spotlight describes the contribution of Suzanne Eaton's pioneering work to our understanding of how morphogens such as Hedgehog can travel over long distances through their association with lipoprotein particles.

Summary: This Spotlight article reviews how Suzanne Eaton, a pioneer in the field of developmental tissue mechanics, connected quantitative cell behavior with physical modeling to reveal fundamental insights into epithelial morphogenesis.

Summary: This Review highlights Suzanne Eaton's pioneering work on the coordination of lipid metabolism with cellular homeostasis and development signalling pathways in the fruit fly Drosophila.

Summary: This Primer gives an overview of the considerations required when planning and conducting transcriptome profiling experiments, from sample isolation to data analysis and display, and discusses best practice in defining gene sets for use in addressing biological questions.

Summary: Retinal organoid systems derived from human pluripotent stem cells are micro-physiological systems that offer new insights into previously unexplored human retina development.

Highlighted Article: Studies of cell organisation and gene expression during human optic fissure closure support a model in which cells at the fissure margins undergo a transient epithelial-to-mesenchymal-like transition that facilitates cell rearrangement.

Summary: Dual-component mechanisms, including activation and repression, govern regeneration enhancer activity to ensure spatiotemporal gene expression during zebrafish heart regeneration.

Summary: Endocannabinoid signalling promotes neuronal maturation and differentiation to deep layer projection neurons in embryonic stem cells and human cerebral organoids via cannabinoid CB₁ receptor activation.

Summary: This study provides genetic tools to precisely mark and examine the dynamic behavior of heterogeneous stem cell populations in the ocular surface epithelium.

Summary: After spinal cord transection, zebrafish oligodendrocytes are transiently lost but re-established by reactive oligodendrocyte progenitor cells.

Highlighted Article: Morphogenesis and specification of cell types are linked by the proneural factor Neurog1, as seen during development of the zebrafish peripheral olfactory organ.

Highlighted Article: Pax6 can induce ectopic eye development in vivo, while retinal organoids can self-organise in vitro. We identify a Pax6 Turing network that could explain both phenomena.

Highlighted Article: Asymmetrical notochord contractility is essential and sufficient to drive embryonic tail bending in chordates, whereas differential epidermis proliferation is a coordinated mechanism that is also required for embryonic tail bending.

Summary: Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms that allow the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised solid organ.

Summary: Experiments using complementary models indicate that disruption of Irf6 and Esrp1/2 in mouse and zebrafish results in orofacial clefts, with an aberrant mesenchymal/epithelial cell population identified in the cleft of the esrp1/2 zebrafish mutant.

Summary: Live-imaging and cell-tracing experiments demonstrate that dynamic epithelial remodeling fuses the anterior and posterior pharyngeal pouches, which are generated independently, into a seamless array of pouches in zebrafish embryos.

Summary: The kinases MPK-1/ERK, GSK-3/GSK3 and CDK-2/CDK2 provide spatial and temporal control of LIN-45/Raf degradation by the ubiquitin ligase SEL-10/FBXW7 during C. elegans vulval precursor cell fate patterning.

Summary: Ovule primordia initiation, which determines the maximum ovule number and subsequent seed number in Arabidopsis, is asynchronous and is regulated by PIN1 polar distribution and the auxin response.

Summary: Mouse embryos display unidirectional movement regulated by muscle contractions, followed by bidirectional movement regulated by LPAR3 signaling, as they space out for implantation.

Summary: The antagonistic balance of GluR subtypes, which is associated with synaptic plasticity, is regulated by cAMP signalling in postsynaptic muscles of Drosophila NMJ synapses.

Highlighted Article: βIII spectrin suppresses the microtubule dynamics at the neuronal dendrite to inhibit abnormal lateral branching, which causes misoriented branch formation.

Summary: HY5 and phytochrome signaling in the shoot modulates coupling between shoot and root thermomorphogenesis. Auxin biosynthesis and signaling form a further regulatory axis for root responses to temperature.

Highlighted Article: Insights into the physical and cellular biology underlying craniofacial development will broaden our understanding of the etiology of orofacial clefts, and guide therapies and prevention strategies for orofacial clefts.

Summary: EPySeg is easy-to-use open-source software that applies deep-learning technology for segmentation of membrane-labelled epithelial tissues, either locally or in the cloud.