Summary: This Review discusses recent research into the role of physical forces, and molecular and cellular mechanisms, driving self-organisation and lineage formation in eutherian mammals.
Summary: Dissecting the mechanisms behind cell fate specification, tissue shape changes and their mutual regulation generates an integrated understanding of embryogenesis. Recent technological advances now make this possible in the human embryo.
Summary: Cell fate decisions are frequently classified as stochastic or deterministic. In this Review, we discuss the meaning and limitations of these terms and illustrate them using recent examples in the literature.
STEM CELLS AND REGENERATION
Summary: Inhibition of BMP signaling reveals its role in limb regeneration and shows that regeneration is, like development, a progressive process.
Identification and requirements of enhancers that direct gene expression during zebrafish fin regeneration
Summary: RNA-seq and ATAC-seq profiling of the dynamic transcriptomic and chromatin accessibility landscape during zebrafish fin regeneration identifies new regulatory elements that direct regeneration-responsive gene expression.
FGF2 modulates simultaneously the mode, the rate of division and the growth fraction in cultures of radial glia
Highlighted Article: When mode and/or rate of division are changing, a branching process, rather than a thymidine analog method, provides temporal resolution, it is more robust and does not interfere with cell homeostasis.
Summary: Self-avoidance prevents dendrite crossover and is important for sensory function. This study identified a cell adhesion molecule that promotes self-avoidance by regulating cytoskeletal assembly in the dendrite.
Cell type- and stage-specific expression of Otx2 is regulated by multiple transcription factors and cis-regulatory modules in the retina
Summary: Retinal cells use transcription factors with different expression patterns and cis-regulatory modules with different chromatin configurations to regulate the cell type- and stage-specific expression of Otx2 precisely.
Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis
Highlighted Article: Morphogenesis and growth rate of all plant organs involve the m6A-binding proteins ECT2, ECT3 and ECT4.
Ecdysone-dependent feedback regulation of prothoracicotropic hormone controls the timing of developmental maturation
Summary: Feedback activation of the neuropeptide PTTH induces a maturation-inducing surge in steroid production that regulates the timing of the juvenile-to-adult transition in Drosophila.
How affinity of the ELT-2 GATA factor binding to cis-acting regulatory sites controls Caenorhabditis elegans intestinal gene transcription
Summary: Transcript levels of the C. elegans asp-1 gene are controlled by the affinity with which the intestine-specific GATA-type transcription factor ELT-2 binds to promoter XXTGATAAXX sites.
Sensory regulated Wnt production from neurons helps make organ development robust to environmental changes in C. elegans
Summary: Sensory perception can increase Wnt production from neurons to reinforce developmental processes, which helps explain how animals can still develop properly when experiencing stress from environmental variations.
C-terminal phosphorylation modulates ERM-1 localization and dynamics to control cortical actin organization and support lumen formation during Caenorhabditis elegans development
Summary: CRISPR/Cas9-generated erm-1 mutant alleles reveal that a PIP2-binding site is crucial for ERM-1 function, and phosphorylation of a C-terminal regulatory threonine modulates its localization and dynamics to control actin organization and support lumen formation in development.
Summary: The time of migration and expansion of the cortical volume are factors influencing the final rostrocaudal differential arrangement of cortical interneurons in the cerebral cortex.
An auxin transport network underlies xylem bridge formation between the hemi-parasitic plant Phtheirospermum japonicum and host Arabidopsis
Highlighted Article: Investigating the auxin response at later stages of haustorium formation provides the first molecular insights into tracheary element differentiation that lead to xylem bridge formation during plant parasitism.
Precise coordination of cell-ECM adhesion is essential for efficient melanoblast migration during development
Summary: Mice without dynamic regulation of cell-to-extracellular matrix adhesion present belly white spotting because of delayed cell migration throughout development.
AMP-activated protein kinase regulates cytoplasmic dynein behavior and contributes to neuronal migration in the developing neocortex
Summary: Knockdown and overexpression studies reveal that AMPK associates with dynein, phosphorylates dynein intermediate chain at Ser81, regulates cytoplasmic dynein behavior and drives nuclear migration in migratory neurons of the developing neocortex.
TECHNIQUES AND RESOURCES
Summary: Defining the transcriptional programs of synovial joint cells in mouse development, including progenitors of tendon, ligament, synovium, meniscus, sheath, and permanent and transient chondrocytes, using single-cell RNA-seq.