Summary: This Primer discusses Hydra as a model system for investigating regeneration, highlighting how the reactivation of developmental processes leads to whole-body regeneration after amputation and also after tissue dissociation and reaggregation of organoid-like structures.
Summary: This Review focuses on the specific interdependence of axons and Schwann cells that enables peripheral nerve development and maintenance.
STEM CELLS AND REGENERATION
Summary: As pluripotent cells undergo neural differentiation they swap E-cadherin for N-cadherin. This switch in adhesion molecules modulates signalling in order to facilitate the differentiation process.
Summary: Although the cyclin-dependent kinase CDK2 is not necessary for spermatogonial function in mice, CDK2 hyperactivity delays establishment of the spermatogonial stem cells and prevents their differentiation.
PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons
Highlighted Article: PLXNA1 and PLXNA3 convey the pathfinding of olfactory and vomeronasal axons as a prerequisite for neuroendocrine neurons to migrate into the hypothalamus and release gonadotropins into the circulation.
Summary: Expressing anti-angiogenic signals in the zebrafish pancreatic islet reveals distinct roles for the vasculature in tissue architecture and function.
Summary: Live imaging of the early Drosophila embryo reveals the dynamics of an extracellular morphogen gradient that undergoes continuous refinement by ligand shuttling, generating a sharp morphogen peak that is essential for patterning gastrulation.
Temporospatial sonic hedgehog signalling is essential for neural crest-dependent patterning of the intrinsic tongue musculature
Summary: Temporospatial sonic hedgehog signalling in neural crest-derived lingual mesenchyme is involved in arrangement of the lingual muscles through tenocyte differentiation.
Crosslinking activity of non-muscle myosin II is not sufficient for embryonic cytokinesis in C. elegans
Highlighted Article: The motor activity of non-muscle myosin II is essential for cytokinesis and contributes to all stages of the process in C. elegans embryos.
Regulation of nuclear-cytoplasmic partitioning by the lin-28-lin-46 pathway reinforces microRNA repression of HBL-1 to confer robust cell-fate progression in C. elegans
Summary: Inhibition of nuclear accumulation and microRNA-mediated repression of protein synthesis cooperate to confer the necessary precision and robustness to temporal downregulation of a transcription factor.
GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126
Summary: GATA2 and its target gene miR-126 are novel regulators of lymphatic endothelial cell junctions, which are important regulators of vascular morphogenesis.
Endocrine and local signaling interact to regulate spermatogenesis in zebrafish: follicle-stimulating hormone, retinoic acid and androgens
Summary: Retinoic acid is important for, and integrated into, the endocrine regulation of zebrafish spermatogenesis; however, in contrast to mammals, is not crucial for spermatogonia differentiation.
Three-dimensional live imaging of Atoh1 reveals the dynamics of hair cell induction and organization in the developing cochlea
Summary: Time-lapse three-dimensional imaging is a powerful method to analyze spatiotemporal gene expression changes of individual cells in inner and outer hair cell fate control of embryonic cochlear epithelium.
TECHNIQUES AND RESOURCES
Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers
Highlighted Article: We have developed a new transgenic technique for simultaneous labelling of all three germ layers in mice that overcomes the difficulties of conventional single-gene tracing systems in precision lineage tracing.