CsTFL1 inhibits determinate growth and terminal flower formation through interaction with CsNOT2a in cucumber
Summary: A novel insight into the molecular mechanisms of determinate growth in cucumber and a strategy for fine-tuning plant architecture using CsTFL1 to adapt to different cucumber production systems.
Summary: This Primer provides an overview of salamanders as a model system for studying regeneration, outlining the mechanisms deployed during salamander regeneration and highlighting how the study of salamanders has increased our understanding of regeneration more broadly.
STEM CELLS AND REGENERATION
Highlighted Article: During primitive endoderm differentiation in mouse, ERK activation is regulated by NANOG, which induces the expression of both Fgf4 and Etv5, providing intrinsic and extrinsic signals at the same time.
Highlighted Article: BMP4 generates the patterning of dorsal spinal cell types in mouse and human pluripotent stem cell-derived organoids.
Nkx2-5 defines a subpopulation of pacemaker cells and is essential for the physiological function of the sinoatrial node in mice
Summary: Mouse lines in which two distinct pacemaker cell populations can be distinguished reveal a novel role for Nkx2-5 in pacemaking function in the sinoatrial node.
Summary: A novel genetic strategy allowing the tracing of axonal projections in mouse brain identifies Rorβ as a key factor regulating axonal targeting to distinct subcortical areas.
Migrating cells control morphogenesis of substratum serving as track to promote directional movement of the collective
Summary: Collectively migrating cells not only respond to extrinsic molecular cues and surrounding topology, but can also physically modify the morphogenesis of their tissue substrate.
Highlighted Article: Monitor lizards, which have the most-pronounced ventricular septation of lizards, express evolutionarily conserved transcription factors and exhibit a specialized manner of electrical activation.
Summary: Dchs1-Fat4 signalling plays a crucial role in osteogenesis by regulating Runx2 activity via Yap and Taz, providing insight into how craniofacial development may be altered in Van Maldegem syndrome.
Highlighted Article: The development of protrusions, such as eminences and condyles, along long bones in mice is coordinated by a patterning mechanism involving global and regional genetic regulation.
Modular tissue-specific regulation of doublesex underpins sexually dimorphic development in Drosophila
Summary: The expression of doublesex in sexually dimorphic structures in Drosophila legs and genitalia is regulated by tissue-specific cis-regulatory elements, providing insight into how cells obtain their sex-specific identity.
Summary: MOZ (KAT6A, MYST3), a histone acetyltransferase and chromatin writer, is essential for normal craniofacial development, where it regulates gene expression, including the distal-less homeobox 5 transcription factor gene.
Summary: The microRNA mir167 limits Arabidopsis anther expansion growth shortly before flowers open. Without this regulation, enlarged anthers do not desiccate enough to dehisce and release pollen.
A whole organism small molecule screen identifies novel regulators of pancreatic endocrine development
Summary: New pdx1 expression modulators are identified in zebrafish, and their ability to induce pancreatic endoderm formation, transdifferentiation of α-cells to β-cells and β-cell maturation is assessed at different stages of pancreas development.
Summary: Hat-trick, a novel interactor of Notch that genetically interacts with Notch pathway components, is a component of the Notch-Su(H) activation complex and positively regulates Notch signaling.
Wheat VRN1, FUL2 and FUL3 play critical and redundant roles in spikelet development and spike determinacy
Summary: The wheat MADS-box proteins VRN1, FUL2 and FUL3 are essential for the initial development of the lateral and terminal spikelets, and control the number of spikelets per spike.
Adaptive correction of craniofacial defects in pre-metamorphic Xenopus laevis tadpoles involves thyroid hormone-independent tissue remodeling
Highlighted Article: Using several different methods of perturbation, we identify molecular and tissue-level factors involved in the adaptive self-correction of malformed craniofacial tissue in the vertebrate Xenopus laevis.
The lymphoid-associated interleukin 7 receptor (IL7R) regulates tissue-resident macrophage development
Highlighted Article: A mouse Il7r-Cre lineage-tracing model, together with blockade of IL7R function and germline deletion of IL7R, provide evidence for the interleukin 7 receptor as a regulator of fetal macrophage development during late gestation.
Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation
Highlighted Article: Rhombomeric boundary cells act as mechanosensors through Yap/Taz-TEAD activity, affecting progenitor maintenance and therefore balancing proliferation and differentiation.
Summary: Physiological and molecular defects in the lacrimal glands of Eda−/− mice reveal that Eda signaling contributes to the cornea/lacrimal gland feedback loop in both basal and reflex tear production.
Sall4 regulates neuromesodermal progenitors and their descendants during body elongation in mouse embryos
Summary: Conditional knockout of Sall4, a stem cell gene, reveals roles in neuromesodermal progenitors and their descendants in post-gastrulation mouse embryos.
TECHNIQUES AND RESOURCES
Summary: We optimize a procedure for enzyme-catalyzed proximity labeling in live Drosophila ovary tissue to interrogate the Drosophila ring canal protein interactome.