Summary: This Primer discusses how instrumental developmental biology has been in unravelling specific aspects of Wnt signalling, and its impact on current clinical research and the development of novel treatments for cancer.
STEM CELLS AND REGENERATION
Oct4 regulates the embryonic axis and coordinates exit from pluripotency and germ layer specification in the mouse embryo
Highlighted Article: Ectopic expression of Nanog, expansion of the presumptive endodermal domain and trapping of cells in the primitive streak ultimately result in consistent disrupted phenotype with loss of embryonic structures.
Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac jelly volume
Summary: nppa/nppb double mutant zebrafish embryos have increased cardiac jelly, which can be abrogated by bmp4 knockdown and places Nppa and Nppb in a regulatory loop with Tbx2, upstream of Bmp.
Highlighted Article: Combinatorial mutagenesis studies reveal that a large family of Forkhead transcription factors integrate diverse signaling outputs to specify distinct cartilages and teeth in the developing zebrafish face.
A non-autonomous function of the core PCP protein VANGL2 directs peripheral axon turning in the developing cochlea
Summary: Planar cell polarity proteins function in cochlear supporting cells to guide peripheral axon guidance and the innervation of outer hair cells by the spiral ganglion.
Developmental vascular regression is regulated by a Wnt/β-catenin, MYC and CDKN1A pathway that controls cell proliferation and cell death
Summary: A model for developmentally scheduled regression of vascular endothelial cells reveals a link between the Wnt/β-catenin pathway and the cell-cycle regulators MYC and CDKN1A.
Yif1 associates with Yip1 on Golgi and regulates dendrite pruning in sensory neurons during Drosophila metamorphosis
Summary: Two novel Drosophila proteins, Yif1 and Yip1, associate with each other and regulate Golgi integrity to act as novel players of dendrite pruning in sensory neurons during early metamorphosis.
Summary: A mathematical model to simulate melanoblast distribution in the embryonic mouse epidermis, based on keratinocyte attraction, melanoblast repulsion and melanoblast memory of their previous path.
Summary: Here, using chick embryos, we showed that post-transcriptional silencing of the Gli3 repressor by miR-133 is required to stably establish the myogenic programme in early somites.
Retinoic acid-induced expression of Hnf1b and Fzd4 is required for pancreas development in Xenopus laevis
Summary: The direct RA target genes Hnf1b and Fzd4 act as mediators of RA function in the pre-patterning of the dorsal endoderm during Xenopus gastrulation, resulting in pancreas specification in this context.
TECHNIQUES AND RESOURCES
Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust
Highlighted Article: In situ hybridization chain reaction (HCR) v3.0 exploits automatic background suppression to enable multiplexed quantitative mRNA imaging and flow cytometry with dramatically enhanced performance and ease of use.
Summary: A new array tomography workflow for model organisms optimizing EM preparation and fluorescence signal preservation, facilitating 3D data acquisition and the correlation of fluorescence and EM data.