IN THIS ISSUE
Summary: This Meeting Review summarises the recent ‘Engineering the embryo’ meeting, at which participants explored how synthetic systems can be used to understand principles of embryonic development.
Summary: This Primer article provides an overview of Krüppel-like factors (KLFs), a family of zinc-finger transcription factors that play fundamental roles in development.
Summary: This Review discusses the different lung stem and progenitor cells that exist during normal homeostasis and that display remarkable lineage plasticity following injury.
Highlighted article: Cytotrophoblasts (CTBs) within the smooth chorion of the human placenta are implicated in severe pre-eclampsia, undergoing significant expansion and changes in gene expression.
STEM CELLS AND REGENERATION
TRBP maintains mammalian embryonic neural stem cell properties by acting as a novel transcriptional coactivator of the Notch signaling pathway
Summary: Transactivation response element RNA-binding protein (TRBP) acts as a transcriptional coactivator of the Notch pathway and regulates neural stem cells during brain development in mice.
Highlighted article: Integrins are required for neoblast migration and the formation of organized tissues, and for restricting neurogenesis during planarian regeneration.
Integrins are required for tissue organization and restriction of neurogenesis in regenerating planarians
Highlighted article: Integrin signaling acts to recruit and localize progenitor cells following injury, thereby promoting the correct organization of regenerating planarian tissue.
Apoptosis restores cellular density by eliminating a physiologically or genetically induced excess of enterocytes in the Drosophila midgut
Highlighted article: Ingestion of low amounts of bacteria induces a mild early stress response that triggers the proliferation of Drosophila midgut stem cells, with excess cells then eliminated by apoptosis.
Summary: PI3-kinase-dependent regulation of DE-cadherin controls cell adhesion between Drosophila neuroblasts and neighboring cortex glia, and between neuroblasts and their daughter cells.
The assembly of developing motor neurons depends on an interplay between spontaneous activity, type II cadherins and gap junctions
Highlighted article: A network of interactions between cadherins, gap junctions and spontaneous activity governs the assembly of cranial motor neurons into functional groups in the chick brainstem.
Summary: Quantitative measurements and computational modelling suggest that dilution of the cytokine Unpaired is a plausible mechanism to explain growth control in the Drosophila eye disc.
Regulatory logic driving stable levels of defective proventriculus expression during terminal photoreceptor specification in flies
Summary: The activities of two cis-regulatory enhancers, which integrate combinatorial transcription factor input, feedback and redundancy, are coordinated to drive dve expression in the Drosophila eye.
Summary: The C. elegans histone demethylase JMJD-1.2 is required non-cell-autonomously for axon guidance and modulates the transcription of Hh-related genes, revealing a role for Hh signaling in C. elegans neural development.
Summary: The cardiac-enriched microRNA miR-1 negatively regulates the E3 ubiquitin ligase Nedd4L, which ubiquitylates many proteins and controls heart development in Drosophila.
Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development
Summary: During trophoblast development in mice, GATA2 and GATA3 act synergistically by directly regulating a large number of common genes, and together are important to ensure trophoblast lineage progression.
Summary: During mammalian blood vessel formation in vivo and in vitro, Flt1 regulates both the frequency of transient contacts that occur before vessels form stable connections and target site selectivity.
Eccentric position of the germinal vesicle and cortical flow during oocyte maturation specify the animal-vegetal axis of ascidian embryos
Summary: Centrifugal force-mediated translocation of the germinal vesicle of a Halocynthia roretzi oocyte to the opposite side of the egg is sufficient to completely reverse animal-vegetal polarity.
Ataxin 2-binding protein 1 is a context-specific positive regulator of Notch signaling during neurogenesis in Drosophila melanogaster
Summary: Drosophila A2BP1 regulates sensory organ specification by potentiating Notch signaling, forming part of the Suppressor of Hairless complex and acting in a context-dependent manner.
A novel floor plate boundary defined by adjacent En1 and Dbx1 microdomains distinguishes midbrain dopamine and hypothalamic neurons
Summary: A novel boundary that subdivides the mouse mesodiencephalic floor plate into two microdomains, each giving rise to distinct types of neurons, is identified.
TECHNIQUES AND RESOURCES
Summary: TALE nucleases are used to target the DDX4 (vasa) locus in chicken primordial germ cells and generate DDX4 knockouts, which provide insights into DDX4 function in early chick development.