IN THIS ISSUE
Summary: This Meeting Review discusses the research that was presented at the ‘Auxin 2016’ meeting, highlighting the latest advances in understanding auxin transport, metabolism and signaling.
Summary: This Review discusses the metabolic remodelling that occurs during the transitions between different states of pluripotency and commitment, both in vitro and in the early mammalian embryo.
Summary: This Review discusses the cellular, molecular and biomechanical mechanisms involved in neural tube closure, focusing on the most recent advances in the field.
STEM CELLS AND REGENERATION
Jmjd2c facilitates the assembly of essential enhancer-protein complexes at the onset of embryonic stem cell differentiation
Highlighted article: Jmjd2c is required for the proper assembly of mediator-cohesin complexes at lineage-specific enhancer regions and is essential for timely gene activation.
A p53-based genetic tracing system to follow postnatal cardiomyocyte expansion in heart regeneration
Highlighted article: Clonal expansion of p53+ cardiomyocytes is observed in neonatal heart, pre-adolescent and adult hearts, as well as in regenerating neonatal hearts subject to three different injury models.
R-spondin 1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways
Summary: R-spondin 1, a Wnt co-factor, regulates the expression of components of the parallel pathways Wnt16/DeltaC/DeltaD and Vegfa/Tgfβ1, required for specification of hematopoietic stem cells.
Summary: A unique transcriptional program that regulates the coordination of positional information and tissue differentiation in regenerating salamander limbs and tails .
Summary: Balanced expression of Nipped-A prevents hyperproliferation of intestinal stem cells in aged Drosophila midguts, identifying Nipped-A as a novel regulator of stem cell proliferation.
Summary: The level of ID4 expression distinguishes stem cells and immediate progenitors in mammalian spermatogonial cells, while downregulation of ID4 is required for transition between the functional states.
Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells
Summary: Optimised strategies and protocols enable a range of complex genetic manipulations directly in mouse and human neural stem cell lines.
Summary: Sequential P-cadherin repression in the primitive streak and the neural plate defines embryonic territories during early chick embryonic development.
Faithful mRNA splicing depends on the Prp19 complex subunit faint sausage and is required for tracheal branching morphogenesis in Drosophila
Summary: Drosophila faint sausage (fas) encodes a subunit of the spliceosome activating Prp19 complex, which is essential for efficient pre-mRNA splicing.
PAPC couples the segmentation clock to somite morphogenesis by regulating N-cadherin-dependent adhesion
Highlighted article: The protocadherin PAPC regulates the segmental de-adhesion of the somites in the developing chick embryo by promoting CDH2 (N-cadherin) endocytosis.
Reconstitution of Torso signaling in cultured cells suggests a role for both Trunk and Torso-like in receptor activation
Summary: Evenly distributed Trunk protein and terminally localized Torso-like combine to promote spatially restricted dimerization and activation of the receptor tyrosine kinase Torso.
Summary: Two parallel sensory lines in medaka share a common origin and are composed of identical organs that are, nevertheless, generated through different morphogenetic programmes.
Tissue-specific regulation of alternative polyadenylation represses expression of a neuronal ankyrin isoform in C. elegans epidermal development
Summary: Selective repression of a neuronal isoform of ankyrin in epidermis depends on alternative polyadenylation at an intronic transcriptional termination site and is necessary for epidermal tissue integrity.
Summary: Time-lapse imaging of zebrafish lens epithelium reveals that cell division and cadherin-mediated adhesion regulate lens epithelial cell movement via modulation of epithelial tension.
TECHNIQUES AND RESOURCES
Summary: Chromatin profiling identifies numerous putative transcriptional enhancers of genes important for sex determination and Sertoli development, and validate a novel enhancer upstream of Wt1.