IN THIS ISSUE
Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos
Summary: This Spotlight article discusses the use of CRISPR-based genome engineering in human embryos and the emerging themes therein.
Summary: ouchless phenotypes are attributed to an essential splice site mutation affecting adgra2 (gpr124) splicing and function, rather than to downregulated sorbs3 transcript levels, as previously thought.
From stem cells to human development: a distinctly human perspective on early embryology, cellular differentiation and translational research
Summary: This Meeting Review summarises the major themes and results presented at the second 'From Stem Cells to Human Development' meeting, held in October 2016.
Summary: This Review discusses how single cell RNA sequencing has been used to study developmental and stem cell biology, providing insights into cell type diversity and developmental dynamics.
Summary: Epidermal fate is autonomously determined by a gene circuit beginning with Sox1/2/3 and Tfap2-r.b in ascidian embryos.
Differential temporal control of Foxa.a and Zic-r.b specifies brain versus notochord fate in the ascidian embryo
Summary: The temporally non-overlapping expression of Foxa.a and Zic-r.b in the brain lineage is regulated by three repressors: Prdm1-r.a, Prdm1-r.b and Hes.a.
Highlighted article: Genital ridge somatic cells have the potential to differentiate into supporting cells or steroidogenic cells depending on Wt1 gene expression.
Highlighted article: Computational modelling reveals that Sachs' rule, animal-vegetal polarization of cell division, cortical rotation and adhesion are the main contributing variables to spiral cleavage.
Rewiring of embryonic glucose metabolism via suppression of PFK-1 and aldolase during mouse chorioallantoic branching
Highlighted article: Metabolic remodelling during E8.5 to E10.5 in mouse redirects glucose carbon into the pentose phosphate pathway, a process partially regulated by Lin28a.
Summary: Ackr2−/− mice display precocious mammary gland development as a result of impaired chemokine scavenging and increased macrophage recruitment to the mammary gland.
Summary: Analysis of PDGFRα gain-of-function mutant mice demonstrates a role in balancing the expansion of fibroblast and adipocyte lineages before birth.
Remodeling of adhesion and modulation of mechanical tensile forces during apoptosis in Drosophila epithelium
Summary: The transient release of tension upon a reduction in the levels of adherens junctions components contributes to apoptotic cell extrusion in Drosophila epithelium.
Summary: Cellular senescence is an intrinsic part of the developmental programme in amphibians and has a conserved role in vertebrate organogenesis.
Interactions between mural cells and endothelial cells stabilize the developing zebrafish dorsal aorta
Summary: Vascular smooth muscle cells are required to help maintain the vascular basement membrane and promote vessel stabilization during development.
Translation repression by maternal RNA binding protein Zar1 is essential for early oogenesis in zebrafish
Summary: Zar1 RNA binding protein represses zona pellucida RNA translation during early oogenesis. zar1 deletion mutants show ER stress and apoptosis in oocytes and result in an all-male phenotype.
The Drosophila Hox gene Ultrabithorax acts in both muscles and motoneurons to orchestrate formation of specific neuromuscular connections
Summary: Ultrabithorax controls correct innervation of ventrolateral muscles by coordinating Wnt4 ligand expression in muscles with the signalling pathway response in motoneurons.
Summary: Epithelial morphogenesis during the transition from the early to late stage of lung development is coordinated by the activity of Wnt signaling.
MS23, a master basic helix-loop-helix factor, regulates the specification and development of the tapetum in maize
Summary: A cascade of basic helix-loop-helix transcription factors guides tapetal cell development in maize anthers, using proteins conserved in Arabidopsis and rice but deployed with distinctive timing.