IN THIS ISSUE
Summary: This Meeting Review summarises the recent ISSCR/CiRA symposium on pluripotency in Kyoto, Japan, and the exciting progress that has been made in the ten years since iPSCs were first reported.
Summary: This Review considers the important, but often overlooked, role of the extracellular matrix in cell migration and tissue morphogenesis in amniotes.
STEM CELLS AND REGENERATION
Highlighted article: The analysis of zebrafish jawbone regeneration reveals important differences between how the skeleton is formed in the embryo and repaired in adults.
Re-epithelialization of cutaneous wounds in adult zebrafish combines mechanisms of wound closure in embryonic and adult mammals
Summary: Chemical treatments combined with live imaging reveal that re-epithelialization of zebrafish wounds utilizes TGFβ/integrin-dependent active keratinocyte crawling as well as epidermal rearrangements.
The EGFR signaling pathway controls gut progenitor differentiation during planarian regeneration and homeostasis
Summary: EGFR signaling promotes the differentiation of stem cell-derived progenitors into mature gut cells during both normal homeostasis and regeneration of the planarian Schmidtea mediterranea.
RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish
Summary: A component of RNA polymerase III, which is responsible for the synthesis of small noncoding RNAs, plays a previously undescribed role in HSPC maintenance during zebrafish embryogenesis.
Highlighted article: During gonad development in Drosophila, Mdr49 acts in the Hh pathway to ensure the sufficient production of processed Hh that, in turn, signals to guide migrating PGCs.
Cadherin 2/4 signaling via PTP1B and catenins is crucial for nucleokinesis during radial neuronal migration in the neocortex
Highlighted article: In radially migrating mouse cortical neurons, cadherin-mediated signaling to the cytoskeleton regulates the forward movement of the nucleus.
Identification of a regulatory domain controlling the Nppa-Nppb gene cluster during heart development and stress
Summary: Regulation of the Nppa-Nppb cluster in normal and stressed hearts involves the concerted action of multiple enhancers and epigenetic changes distributed across a structurally rigid regulatory domain.
Nanos-mediated repression of hid protects larval sensory neurons after a global switch in sensitivity to apoptotic signals
Summary: The translational repressors Nanos and Pumilio provide a crucial neuroprotective regulatory layer to ensure that Drosophila neurons behave appropriately in response to developmental cues.
Summary: In the mouse corneal epithelium, primary cilia are confined to basal cells and modulate Notch signaling and cell proliferation to maintain normal epithelial thickness.
Fat3 and Ena/VASP proteins influence the emergence of asymmetric cell morphology in the developing retina
Summary: Time-lapse imaging and biochemical studies suggest that Fat3 acts autonomously via Ena/VASP to orient the cytoskeleton in mouse amacrine cell precursors as they migrate and elaborate dendrites.
Syk kinases are required for spinal commissural axon repulsion at the midline via the ephrin/Eph pathway
Summary: Syk kinases regulate the switch in growth cone behaviour, from attraction to repulsion, during spinal cord midline crossing in developing mouse embryos.
Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by Dact1/2
Summary: In Xenopus and chick embryos, the scaffolding activity of DACT1/2 is required for the local and transient inhibition of Wnt/β-catenin activity, a prerequisite for neural crest delamination.
Summary: In developing mouse embryos, Tcf7l1 defines the border between the neural crest and the prospective forebrain via restriction of the Wnt/β-catenin signaling gradient.
Summary: 3D analyses of cardiac valve formation in zebrafish illustrate the complex cellular rearrangements that occur during this process in vivo.
BMP regulates regional gene expression in the dorsal otocyst through canonical and non-canonical intracellular pathways
Summary: BMP signaling regulates the expression of Dlx5 and Hmx3, transcription factors essential for vestibular development, through the canonical pSMAD pathway and a non-canonical pathway, respectively.
TECHNIQUES AND RESOURCES
Summary: CARS microscopy provides a novel and non-invasive method of quantifying lipid content, type and spatial distribution in living mouse oocytes and pre-implantation embryos.