Special Issue: Imaging Development, Stem Cells and Regeneration
Summary: This Primer explains and provides advice on two- and three-dimensional stress inference, an increasingly popular technique to identify the role of mechanics during embryo development.
Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo
Summary: Use of the SunTag method to allow single-molecule imaging in fixed and live Drosophila embryos reveals translational repression of zygotic hunchback mRNAs.
Summary: Oriented cell movement at the leading edge mesendoderm surface allows this tissue to move by being pulled forward by cells at its front end.
TECHNIQUES AND RESOURCES
Summary: Introducing an easy-to-use automated anatomical phenotyping pipeline for mouse embryos along with a highly-detailed anatomical E14.5 atlas.
A landmark-free morphometrics pipeline for high-resolution phenotyping: application to a mouse model of Down syndrome
Summary: This paper presents a new computational pipeline for comparing and mapping global and local anatomical shape differences that is higher-resolution and requires less expertise and labour than traditional landmarking methods.