miR-137 targets Myc to regulate growth during eye development Open Access

During development, regulation of gene-expression is key to cellular homeostasis. Gene-expression regulation by non-coding RNAs (ncRNA) involves prevention of mRNAs accumulation or block translation of their target gene. In a forward genetic-screen to identify the microRNA (miRNA) involved in the growth and patterning of Drosophila eye, we identified a highly conserved miR-137. Gain-of-function of miR-137 results in reduced-eye phenotype by downregulating retinal determination and differentiation markers, and by upregulating negative regulators of eye development like Wingless (Wg) and Homothorax (Hth). Loss-of-function of miR-137 results in an enlarged-eye phenotype. Using bioinformatics- and genetic- approaches, we identified oncogene Myc as the target of miR-137. Gain-of-function of Myc can rescue the reduced-eye phenotype of miR-137 gain-of-function or vice-versa. We tested the role of miR-137 in regulating Myc levels in Ras^V12;scrib^RNAi, a tumor model of oncogenic cooperation that results in neoplastic tumors. Gain-of-function of miR-137 in Ras^V12;scrib^RNAibackground significantly reduced tumor phenotype as well as Myc levels in the eye. Our studies highlight miR-137 as a new posttranscriptional regulator of Myc and a promising therapeutic target for diseases associated with Myc-accumulation.