One of the key tissue movements driving closure of a wound is re-epithelialisation. Earlier wound healing studies have described the dynamic cell behaviours that contribute to wound re-epithelialisation, including cell division, cell shape changes and cell migration, as well as the signals that might regulate these cell behaviours. Here, we use a series of deep learning tools to quantify the contributions of each of these cell behaviours from movies of repairing wounds in the Drosophila pupal wing epithelium. We test how each is altered following knockdown of the conserved wound repair signals, Ca2+ and JNK, as well as ablation of macrophages which supply growth factor signals believed to orchestrate aspects of the repair process. Our genetic perturbation experiments provide quantifiable insights regarding how these wound signals impact cell behaviours. We find that Ca2+ signalling is a master regulator required for all contributing cell behaviours; JNK signalling primarily drives cell shape changes and divisions, whereas signals from macrophages regulate largely cell migration and proliferation. Our studies show AI to be a valuable tool for unravelling complex signalling hierarchies underlying tissue repair.
AI reveals a damage signalling hierarchy that coordinates different cell behaviours driving wound re-epithelialisation
Equal senior authors
Pathway to Independence Programme: our 2024 PI fellows
Following a successful pilot year in 2023 with a fantastic set of postdocs, we are delighted to announce our second cohort of Pathway to Independence (PI) fellows, who we will be supporting with training, mentoring and networking opportunities over the coming years.
Development presents…
Development is excited to host a webinar series showcasing the latest developmental biology and stem cell research. The webinars are chaired each month by a different Development Editor, who invites talks from authors of exciting new papers and preprints. Visit Development presents... on the Node to see which topics are coming up and to catch up on recordings of past webinars.
40 years of the homeobox
2024 marks the 40th year since the discovery of the homeobox in 1984, a landmark that fundamentally impacted several fields including genetics, developmental biology, neuroscience and evolution. To celebrate this anniversary, Development has commissioned a series of articles from leaders in the field demonstrating the impact of the homeobox discovery on different disciplines.
Biologists @ 100 - join us in Liverpool in March 2025
We are excited to invite you to a unique scientific conference, celebrating the 100-year anniversary of The Company of Biologists, and bringing together our different communities. The conference will incorporate the Spring Meetings of the BSCB and the BSDB, the JEB Symposium Sensory Perception in a Changing World and a DMM programme on antimicrobial resistance. Find out more and register your interest to join us in March 2025 in Liverpool, UK.
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