The development of three cell lines initiated from the late embryonic stages of Drosophila melanogaster is described. The primary cultures consisted of trypsinized fragments from embryos 20–24 h old. The length of time between primary culture and subsequent subculture varied from 8 months for the first line to 3 weeks for the third. All three lines have been maintained in vitro for more than a year. The characteristics of each line are given and evidence is presented that at least one line is derived from imaginal disc cells. A few comments on insect tissue culture in general are also made.
Skip Nav Destination
Journal Article| 01 April 1972
Cell lines derived from late embryonic stages of Drosophila melanogaster
Department of Entomology, Walter Reed Army Institute of Research
1Author's address: Department of Entomology, Walter Reed Army Institute of Research, Washington, D.C. 20012, U.S.A.
Received: 13 Sep 1971
Online Issn: 1477-9129
Print Issn: 0950-1991
Copyright © 1972 by Company of Biologists
- Views Icon Views
- Share Icon Share
- Search Site
Imogene Schneider; Cell lines derived from late embryonic stages of Drosophila melanogaster. Development 1 April 1972; 27 (2): 353–365. doi: https://doi.org/10.1242/dev.27.2.353
Download citation file: