There was an error in Development (2025) 152, dev204497 (doi:10.1242/dev.204497).
The legend for Fig. 2 was incorrect. The corrected and original legends are shown below.
Corrected:
Fig. 2. Identification of transcripts upregulated or downregulated during karyogamy. (A) Schematic diagram of karyogamic progression during early zygotic development. Pink, green, and orange circles indicate the egg, sperm, and zygotic/embryonic nuclei, respectively. (B) Karyogamic progression in rice zygotes. A sperm cell expressing the H2B-GFP fusion protein was fused with a wild-type egg cell. (C) Two-celled embryo at 1 day after fusion. Top, middle, and bottom panels in B,C present fluorescent, merged fluorescent and brightfield, and brightfield images, respectively. Scale bars: 20 μm. (D-I) Scatter plots of log10(TPM+1) values for egg cells and zygotes. Red and blue dots represent upregulated and downregulated DEGs, respectively. N.S., transcripts not selected as DEGs (TPM>0 in both or either egg cells or zygotes). The number of transcripts is presented in parentheses.
Original:
Fig. 2. Identification of transcripts upregulated or downregulated during karyogamy. (A) Schematic diagram of karyogamic progression during early zygotic development. Pink, green, and orange circles indicate the egg, sperm, and zygotic/embryonic nuclei, respectively. (B) Karyogamic progression in rice zygotes. A sperm cell expressing the H2B-GFP fusion protein was fused with a wild-type egg cell. (C) Two-celled embryo at 1 day after fusion. Top, middle, and bottom panels in B,C present fluorescent, merged fluorescent and brightfield, and brightfield images, respectively. Scale bars: 20 μm. (D-I) Scatter plots of log10(TPM+1) values for egg cells and zygotes. Red and blue dots represent upregulated and downregulated DEGs, respectively. N.S., transcripts not selected as DEGs (TPM>0 in both egg cells and zygotes at each stage). The number of transcripts is presented in parentheses.
The legend for Fig. 3 was incorrect. The corrected and original legends are shown below.
Corrected:
Fig. 3. Initiation of de novo gene expression in the earliest zygote stage. (A) Set visualization of upregulated DEGs in each zygote stage. Orange and green asterisks indicate persistent and stage-specific upregulation, respectively. (B) Transcripts exhibiting persistent upregulation extracted from the data shown in A. These categories were designated as P1-P5. (C) Heat maps for TPM values of P1 transcripts in sperm cells, egg cells, and early zygotes. Upper and lower clades indicate possible de novo expression and sperm cell-enriched transcription, respectively. (D) Transcripts exhibiting stage-specific upregulation extracted from the data shown in A. These categories were designated as S1-S6. (E) Heat maps for TPM values of S2 transcripts in sperm cells, egg cells, and early zygotes. (F,G) Expression patterns of representative P1 (F) and S2 (G) transcripts confirmed by semi-quantitative RT-PCR. Ubiquitin was used as an internal control. Numbers in parentheses indicate the number of PCR cycles.
Original:
Fig. 3. Initiation of de novo gene expression in the earliest zygote stage. (A) Set visualization of upregulated DEGs in each zygote stage. Orange and green asterisks indicate persistent and stage-specific upregulation, respectively. (B) Transcripts exhibiting persistent upregulation extracted from the data shown in A. These categories were designated as P1-P5. (C) Heat maps for TPM values of P1 transcripts in sperm cells, egg cells, and early zygotes. Upper and lower clades indicate possible de novo expression and sperm cell-enriched transcription, respectively. (D) Transcripts exhibiting stage-specific upregulation extracted from the data shown in A. These categories were designated as S1-S5. (E) Heat maps for TPM values of S2 transcripts in sperm cells, egg cells, and early zygotes. (F,G) Expression patterns of representative P1 (F) and S2 (G) transcripts confirmed by semi-quantitative RT-PCR. Ubiquitin was used as an internal control. Numbers in parentheses indicate the number of PCR cycles.
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The authors apologise for these errors, which do not impact the results and conclusions of the paper.
