There was an error in Development (2019) 146, dev172932 (10.1242/dev.172932).

The authors informed us that during the preparation of Fig. 1B, two photographs of the same pistil (Col-0 23°C x Col-0 16°C and hyl1-2 23°C x hyl1-2 16°C), taken with different gain parameters, were inadvertently included in the published panel. The authors reviewed the original photographs, all showing the same result as the published images, and have corrected Fig. 1B with the appropriate image corresponding to hyl1-2 23°C x hyl1-2 16°C pistils (below).

Fig. 1 (corrected).

Reversion of the phenotypes of miRNA biogenesis mutants at reduced temperatures. (A) Phenotypes of wild-type Col-0 and mutant plants at the vegetative stage grown at 23°C or 16°C. Scale bar: 1 cm. (B) Col-0 or hyl1-2 flowers from plants grown at 16°C or 23°C during the reproductive phase artificially pollinated with pollen from plants grown under the same conditions. Sixteen hours after pollination, pollen viability and pollen tube growth were visualized using Aniline Blue staining. Scale bar: 0.15 mm. (C) Dissection of Col-0 and mutant siliques showing a larger degree of embryonic abortion in hyl1-2 and se-3 mutants grown at 23°C when compared with 16°C. Examples of aborted embryos are indicated by red arrowheads. Magnification is shown in Fig. S1C. Scale bars: 1 mm. (D) Quantification of the percentage of aborted embryos in wild-type and mutant plants grown at 23°C and 16°C. For each genotype, 50 siliques were dissected and embryo abortion was counted. Embryonic lethality is given as the percentage of aborted embryos over the total number of embryos. Error bars show 2×s.e.m. (E) Seed production of different miRNA-related mutants. Plants grown at 23°C for their entire life cycle (light grey), at 16°C for their entire life cycle (white) and at 16°C during the vegetative phase, and then transferred to 23°C for the reproductive phase (black bars), or vice versa (dark grey). The total seed weights of 50 pooled plants are expressed relative to the seed weights of the same mutants grown at 23°C for the entire lifecycle.

Fig. 1 (corrected).

Reversion of the phenotypes of miRNA biogenesis mutants at reduced temperatures. (A) Phenotypes of wild-type Col-0 and mutant plants at the vegetative stage grown at 23°C or 16°C. Scale bar: 1 cm. (B) Col-0 or hyl1-2 flowers from plants grown at 16°C or 23°C during the reproductive phase artificially pollinated with pollen from plants grown under the same conditions. Sixteen hours after pollination, pollen viability and pollen tube growth were visualized using Aniline Blue staining. Scale bar: 0.15 mm. (C) Dissection of Col-0 and mutant siliques showing a larger degree of embryonic abortion in hyl1-2 and se-3 mutants grown at 23°C when compared with 16°C. Examples of aborted embryos are indicated by red arrowheads. Magnification is shown in Fig. S1C. Scale bars: 1 mm. (D) Quantification of the percentage of aborted embryos in wild-type and mutant plants grown at 23°C and 16°C. For each genotype, 50 siliques were dissected and embryo abortion was counted. Embryonic lethality is given as the percentage of aborted embryos over the total number of embryos. Error bars show 2×s.e.m. (E) Seed production of different miRNA-related mutants. Plants grown at 23°C for their entire life cycle (light grey), at 16°C for their entire life cycle (white) and at 16°C during the vegetative phase, and then transferred to 23°C for the reproductive phase (black bars), or vice versa (dark grey). The total seed weights of 50 pooled plants are expressed relative to the seed weights of the same mutants grown at 23°C for the entire lifecycle.

In addition, the right miR164 blot in Fig. 2B was inadvertently duplicated from miR171. The correct blot, which shows the findings stated in the paper, appeared mistakenly as a hidden layer in the final composited figure. The authors have reviewed the original blots to confirm the results and created a corrected Fig. 2B with the appropriate original image (below).

Fig. 2 (corrected).

miRNA production in mutants grown at different temperatures. (A) RNA blots for detecting miRNAs in Col-0, hyl1-2 and se-3 plants grown at different temperatures. U6 was used as a loading control. (B) RNA blots for detecting miRNAs in Col-0, ago1-25 and hen1-5 plants grown at different temperatures. U6 was used as a loading control. (C,D) RNA blots for detecting miRNAs in Col-0, hyl1-2 and se-3 plants grown at 23°C for 5 days and then transferred to 16°C for a given period of time (left blots, D) or grown at 16°C for 5 days and then transferred to 23°C for a given period of time (right blots). U6 was used as a loading control. Samples from different mutants plants were loaded in the same gel with a 15 min interval to facilitate comparison. In all panels, signal intensity was calculated using ImageJ and is expressed relative to the samples at 23°C for each genotype (A,B) or to the time point 0 of each genotype (C,D).

Fig. 2 (corrected).

miRNA production in mutants grown at different temperatures. (A) RNA blots for detecting miRNAs in Col-0, hyl1-2 and se-3 plants grown at different temperatures. U6 was used as a loading control. (B) RNA blots for detecting miRNAs in Col-0, ago1-25 and hen1-5 plants grown at different temperatures. U6 was used as a loading control. (C,D) RNA blots for detecting miRNAs in Col-0, hyl1-2 and se-3 plants grown at 23°C for 5 days and then transferred to 16°C for a given period of time (left blots, D) or grown at 16°C for 5 days and then transferred to 23°C for a given period of time (right blots). U6 was used as a loading control. Samples from different mutants plants were loaded in the same gel with a 15 min interval to facilitate comparison. In all panels, signal intensity was calculated using ImageJ and is expressed relative to the samples at 23°C for each genotype (A,B) or to the time point 0 of each genotype (C,D).

The authors apologize for these errors, which do not impact the results and conclusions of the paper.