The serine protease Corin is a novel modifier of the agouti pathway

The hair follicle is an important model system for the study of organogenesis: in part, because of the attributes that make it amenable to study during follicular neogenesis in the embryo and newborn animal; and, in part, because the lower portion of the hair follicle is regenerated from comparatively well-defined keratinocyte stem cells in the adult. In both neogenesis and regeneration, inductive signaling between epithelium and mesenchyme directs morphogenesis of the follicle. In the embryo, pluripotent keratinocytes in the basal epidermis initiate follicular development by forming a thickened epidermal placode in response to inductive signals from the dermis. Signals from the placode recruit dermal cells to aggregate beneath it and form the dermal condensation, which in turn acquires additional inductive properties. These include signals required for the growth of the placodal keratinocytes and their invagination to form the hair peg that extends into the dermis. The dermal condensate further differentiates into the dermal papilla (DP), which is engulfed by the extending hair peg and comes to lie in the center of the hair bulb at the base of the mature follicle.

During the active growth phase, the mature hair follicle is composed primarily of keratinocytes arranged in concentric layers of differentiated cell types that comprise the hair shaft (HS), inner root sheath (IRS) and outer root sheath (ORS). Growth of the hair occurs as proliferating cells in the hair matrix at the base of the follicle generate additional constituents of the inner layers of the follicle that are organized into the IRS and HS and extruded through the ORS towards the surface of the skin. The DP plays a central role in this process. The keratinocytes abutting the DP act as stem cells of the hair bulb, undergoing asymmetrical divisions to generate transit amplifying (TA) progeny, which then undergo a few divisions before terminally differentiating (Legue and Nicolas,2005). Ablation and grafting studies have demonstrated that the DP is required to maintain the growth of the hair follicle and suggest that it plays an instructive role in driving morphogenesis of the hair(Jahoda et al., 2001; Oliver and Jahoda, 1988).

This period of sustained hair growth lasts for a few weeks in the mouse. At the end of this growth or anagen phase, proliferation in the hair bulb ceases. During the degeneration or catagen phase, the matrix cells either terminally differentiate or apoptose, and the basal end of the hair shaft becomes anchored in the upper follicle. The outer root sheath from the lower two-thirds of the follicle degenerates and the DP is drawn to the base of the permanent portion of the follicle. A quiescent or telogen phase ensues that may last from a few days during the first hair cycle to many weeks in mature animals. At the end of the telogen phase, keratinocyte stem cells resident in the bulge region of the permanent follicle are activated and regenerate the lower portions of the anagen follicle(Morris et al., 2004). It is thought that the DP plays a central role in the activation of these keratinocytes and the subsequent guidance of their proliferation and differentiation to regenerate the follicle, although the nature of that role remains to be empirically defined (Sun et al., 1991).

In addition to its functions in follicle morphogenesis and cycling, the DP also regulates pigmentation of the hair. Pigment is synthesized by melanocytes resident in the hair bulb and deposited in the keratinocytes of the hair medulla and cortex. Pigment production in melanocytes is regulated in part by the activity of the Mc1r receptor expressed on their surface. When this receptor is active, the black pigment eumelanin is produced and deposited in the hair shaft. Mc1r has a basal level of constitutive activity in the absence of its agonists melanocortins that is further stimulated by the binding of these ligands (Barsh, 1999; Smart and Low, 2003). Although the DP is likely to play a role in generating an environment that attracts and maintains melanocytes in the hair bulb(Yoshida et al., 1996), it also regulates hair pigmentation more directly by expressing the agouti signaling protein, hereafter referred to as agouti(Millar et al., 1995). Binding of agouti to Mc1r reduces its activity and results in a shift from the production of eumelanin to the synthesis of pheomelanin, a yellow pigment(Barsh et al., 2000; Chai et al., 2003). Agouti (also known as nonagouti or ASIP) is expressed transiently in the DP of the dorsal pelage during the early growth phase of the hair cycle. The resultant provisional switch to pheomelanin deposition generates a subapical yellow band in the otherwise black hair that defines the agouti coat color. Despite the predominance of black pigment in the hair of agouti mice, the presence of lighter pigment in the hair tip creates the overall appearance of a mottled brown hair coat that provides adaptive coloration in the natural environment. Modest variations in the length of this apical pheomelanin band can dramatically alter coat appearance and represent one mechanism by which adaptive changes in coat color can occur(Hoekstra, 2006).

Partly as a result of the development of mouse strains that allow the manipulation of gene expression in the embryonic precursors of the epidermis and follicular epithelium (Byrne et al.,1994; Indra et al.,1999), the epidermal placode(Levy et al., 2005) or the stem cells of the adult hair follicle (Ito et al., 2005), significant progress has been made in understanding the genetic mechanisms that direct follicle formation in its keratinocyte constituents. Despite its functions in maintaining the niche for matrix stem cells and in organizing both the morphogenesis and pigmentation of the hair follicle, the molecular genetics of DP formation and function have remained less accessible to study because of a comparative lack of tools to purify these cells or manipulate gene expression in vivo. To address this need, we evaluated genes preferentially expressed in the DP of the hair follicle to identify those whose expression in the skin is restricted to this population. Here we report the identification of Corin as a gene specifically expressed in the DP of the hair follicle. Ablation of Corin activity in the DP reveals that it is not required for hair follicle morphogenesis and confirms that the Corin gene is well suited to serve as a platform for the manipulation of gene expression in the DP in vivo. This analysis also reveals an unexpected function for this transmembrane serine protease in the regulation of hair shaft pigmentation and identifies a novel mechanism by which the DP directs the pattern of pigmentation in the hair shaft.

Using a 129SvJ ES-cell line, 64bp downstream of the ATG in exon1 of Corin was replaced by a YFP-Neo cassette. Chimeric mice(A^w/A^w), derived from correctly targeted ES clones, were crossed with C57BL6J (a/a) to generate F0 mice heterozygous for both Corin and agouti (A^W/a). F0 mice were crossed with FVB (A/A) to generate F1 mice (either A^W/A or A/a). Phenotypic analysis was performed on offspring from matings of F1 Corinheterozygous mice. Qualitatively similar results were obtained when the Corin allele was first moved to FVB, C57BL6J or 129 backgrounds for at least six generations.

Non-radioactive in situ hybridization of sections from embryos and dorsal skins were performed with probes corresponding to nts 682-1252 of Corin (GenBank Acc. No. NM_016869) and nts 126-613 of Agouti(NM_015770). Anti-Corin antibodies (1:800) raised in rabbits(Fig. 2A), were detected with FITC-conjugated secondary antibody (1:250) (Jackson) in the presence of TO-PRO-3 (1:40,000).

The analysis of the length of the subapical yellow band was performed on six wild-type and seven mutant postnatal day (P)20 mice. At P20, hairs derived from the first hair cycle are fully grown and are the only hairs present in the dorsal pelage. For each mouse, approximately 400 hairs from mid-dorsal pelage were randomly mounted on slides in a thin layer of Gelvatol. Awl hairs represent approximately 10% of the total hair population. Therefore, about 80 awl hairs per mouse were specifically collected for analysis. Two-tailed unpaired t-tests were performed.

Hair shafts were photographed in bright field and in green fluorescent channel at 100 × magnification. Lack of black pigment in the hair shaft results in autofluorescence that corresponds with the deposition of yellow pigment. The green channel of the fluorescent image was duplicated in the red channel to generate a yellow color. The modified fluorescent image was overlaid on the bright field image in PhotoShop and reduced to 45%opacity.

Mid-dorsal skins from P0-P9 mice were collected and used to prepare RNA with Trizol solution (Invitrogen). The total RNA was further purified using RNeasy mini kit (Qiagen) and a DNase I digestion step. Normalized RNA was reverse transcribed using random hexamer primers. Primer pairs (Superarray)for Actb (PPM02945A), Corin (PPM41062A), Agouti(PPM24722A), Mc1r (PPM04903A), Pomc (PPM37114A), Atrn (PPM30947A) and Mgrn1 (PPM02945A) and CYBR Green-Fluorescein PCR Mater Mix were used with an iCycler (Bio-Rad), MyiQ Single-color Detection system, MyiQ Optical System Software. Differences between samples were quantified based on the ΔΔCt method. The number of mice per genotype (WT/Mut) per stage used in this expression profile was as follows: P0(5/3); P1(9/10); P2(13/10); P3(8/7); P4(13/9); P5(7/11);P6(5/6); P7(3/4); P8(2/2); P9(4/5). The analysis was performed individually for each mouse and the average values were calculated for each group of mice. Thus, the s.d. reflects the variation in gene expression between individual mice in the same group.

As an initial step towards identifying genes that might serve as platforms for the study of DP function, we used a mouse expressing EGFP under the control of a short segment of the human versican promoter(Kishimoto et al., 2000). A population enriched in DP cells was purified from newborn skin on the basis of GFP expression (Shimizu and Morgan,2004). Gene expression in these cells immediately after isolation was compared with that of cells maintained in culture with the expectation that genes expressed in the DP as a consequence of inductive signaling from the follicular keratinocytes would be preferentially lost from the cultured cell expression profile. Among the genes identified in this way was the Corin locus. Corin transcripts were readily detected in freshly isolated cells but dropped below the level of detection within 24 hours in culture (see Fig. S1 in the supplementary material). Corinencodes a transmembrane protease that is expressed in the heart and participates in blood pressure regulation by cleaving the prohormone proANP to its active form (Chan et al.,2005; Yan et al.,2000). EST expression profiles and available expression data suggested that Corin expression was largely restricted to cardiomyocytes (see also Yan et al.,1999). This restricted expression profile and the expression of the Corin protein on the surface of the cell made this gene an attractive candidate for further investigation of its expression and function in the skin. Although mice lacking Corin have the altered blood pressure regulation and cardiovascular defects predicted by its role in cleaving ANP, no skin or hair phenotypes were reported in these mice(Chan et al., 2005).

Hair follicle formation occurs in three waves during embryogenesis, which initiate at E14, E16 and between E18 to birth. These waves give rise to follicles that generate distinct hair types. Follicles that give rise to the long, straight guard hairs arise in the first wave, whereas the intermediate-sized awl and smaller zigzag and auchene hair types are thought to arise in the second and third waves, respectively. Corinexpression at the RNA level was examined in embryonic skin and in the dorsal pelage of post-natal animals by in situ hybridization(Fig. 1). It is first detected in the skin in the nascent dermal condensate at embryonic day (E)15, as the first wave of follicles form (Fig. 1A). Its expression persists in the DP throughout the development of these follicles and is also observed in the dermal condensate and DP of the second and third waves of follicles throughout their morphogenesis(Fig. 1B,C). Corin transcripts were not detected elsewhere in the skin at any stage examined. Corin expression persists and remains restricted to the DP throughout the anagen(growth) phase of the hair cycle (Fig. 1D-G and data not shown). Expression is not detected during the catagen (regression) or telogen (resting) phases but returns with the onset of a new anagen phase (data not shown). Corin is expressed in a similar pattern in all follicle types of the dorsal pelage.

Antisera were generated to evaluate Corin protein distribution in the skin. A schematic representation of the inferred protein structure is shown in Fig. 2A. In addition to the C-terminal protease domain, this type II transmembrane protein has two cysteine-rich `frizzled homology' domains, two series of LDLR-like repeats and a scavenger receptor conserved region. A segment corresponding to LDLR repeats 1-5 was expressed in bacteria and used to immunize rabbits. The resultant antisera revealed Corin expression in the DP of the hair follicle(Fig. 2B-F). Corin protein levels mirror the expression pattern of Corin at the RNA level,suggesting that any post-transcriptional events controlling Corinactivity and function do not include the participation of translational regulation. Furthermore, consistent with the predicted protein structure, the majority of Corin protein detected remains associated with DP cells(Fig. 2E,F). Although detailed analysis was restricted to dorsal pelage, Corin was also detected in the DP of vibrissae and pelage follicles of the ventral skin.

A null allele of Corin was generated by inserting a YFP-Neo cassette into the first exon of Corin that harbors the initiation codon and encodes most of the intracellular N-terminal domain(Fig. 3A). Mice homozygous for this allele (Corin^-/-) were recovered at expected frequencies and were viable and fertile(Fig. 3B-D). Immunostaining with anti-Corin antibodies confirmed both the absence of detectable Corin protein in these mice and the specificity of the anti-sera used in this study(Fig. 3E,F). In P3 skin, the three waves of follicle formation are represented by follicles in different stages of development. All three stages of hair follicles are present in mutant skin with frequencies similar to that of the wild type, suggesting that hair follicle initiation is normal in homozygous mutants(Fig. 3E,F, see Fig. S2 in the supplementary material). Indeed, the hair coat in homozygous mutant animals appears normal with respect to numbers, frequency, structure and growth rate at all stages examined (Fig. 4and see Fig. S3 in the supplementary material). Corin activity is not required for the normal morphogenesis or cycling of the hair follicle.

However, there is a striking difference between the wild-type and mutant pelage. Homozygous mice exhibit a distinctly lighter coat color that is most pronounced in juveniles but persists through adulthood(Fig. 4A,B). This phenotype is dependent on the presence of a functional allele (A) of agouti. Corin mutants homozygous for a null allele of the agouti gene(a/a) are black and indistinguishable from the wild type(Fig. 4C), whereas Corin mutants on A/a or A/A backgrounds are progressively lighter than corresponding mice with a wild-type Corinallele (Fig. 4D). The yellow appearance is a result of pheomelanin production and not alteration of the diffractive properties of the hair, because tryosinase mutants incapable of synthesizing melanin have white fur irrespective of the presence or absence of Corin (Fig. 4C). Heterozygotes for Corin are indistinguishable from the wild type on all agouti backgrounds tested (data not shown).

To gain more insight on the mechanism underlying the coat color phenotype,hair shafts were plucked from the back skin of wild-type and mutant mice at the end of the first hair cycle. The four types of hair, guards, awls, zigzags and auchenes, differ in the extent of pheomelanin content. Changes in hair type could in principle explain the lighter coat color. However, all four types of hair were present at similar frequencies in both wild-type and mutant mice (see Fig. S3 in the supplementary material). Corin is not required to specify hair type.

The length of the subapical yellow band was analyzed separately for each hair type. Zigzag hairs were the most abundant hair type and have a prominent yellow band. Regardless of Corin genotype, all zigzag hairs exhibited a yellow band that was restricted to the apical segment of the hair. In mutant zigzag hairs, the length of the yellow band was increased. Fig. 5A shows extreme examples of wild-type and mutant zigzag hairs: in the absence of Corin the yellow band extended for the length of the terminal segment, whereas in the wild type it extended less than half of that length. Both the length of the terminal segment and the length of the yellow band vary between different zigzag hairs on the same mouse. To quantify the differences between wild-type and mutant zigzag hairs, the ratio (R) between the length of the yellow band (Y) and the length of the apical segment (Z) was calculated(Fig. 5B). Hairs were scored for the presence or absence of a black tip and assigned to one of three categories: R≤0.5, 0.5<R≤0.75, or 0.75<R≤1(Fig. 5C-H). This analysis revealed two major differences (see Fig. 5I). First, the basal extension of the yellow band increased substantially in the mutant. The number of zigzag hairs with R>0.5 increased significantly from 33% in the wild type to 85% in mice lacking Corin, and the category exhibiting R>0.75 increases from 0 to 32%. Second,the yellow band was also extended in the apical direction in most hairs. Although virtually all of the wild-type hairs had a terminal black tip, 70% of the mutant hairs did not (see Fig. S4 in the supplementary material). It is striking that the extension of the yellow band in the apical and basal directions are to some extent independent events. Hairs with the longest yellow bands may nevertheless have a black tip, whereas hairs in the shortest category may have yellow tips. Despite the breadth of the categories used in this analysis, the pigmentation pattern of a minimum of 85% of the zigzag hairs is altered in the Corin mutant.

Similar analysis with some minor modifications was performed for the awl hairs (Fig. 6). The ratio (R)between the length of the yellow band (Y) and the length of the whole hair (A)was calculated (Fig. 6B). In contrast to zigzag hairs, many of the wild-type awl hairs were completely black (R=0, Fig. 6C). Furthermore, regardless of Corin genotype, all awl hairs terminated with a black tip. Two additional categories were defined: R≤0.25 and 0.25<R≤0.5. Representative examples of all three categories are shown in Fig. 6A. A striking reduction in the frequency of completely black awl hairs from 61% in the wild type to 28% in the mutant was observed (Fig. 6C). In the absence of Corin, many awl hairs that otherwise would be completely black were transformed to include a subapical yellow band. Furthermore, the percentage of awl hairs with 0.25<R≤0.5 increased from 7% in the wild type to 54% in mutant mice. This increase (47%) exceeds the proportion that could be contributed by awl hairs that normally exhibit a yellow band in wild type (39%) and demonstrates that hairs that would normally lack any discernible agouti activity show pheomelanin deposition over a quarter of their length in the absence of Corin.

A similar elongation of the yellow band was observed in auchene hairs as a result of Corin ablation (see Fig. S5 in the supplementary material). As observed in zigzag hairs, the extension of the pheomelanin band in the apical and basal directions were independent events. Although substantial extension in the basal direction was observed, all mutant auchene hairs end with black tips as observed in wild-type mice. In contrast to zigzags, awls and auchenes, all guard hairs were completely black in both wild-type and mutant mice, suggesting that agouti signaling is completely inactive in this type of hair, regardless of the presence or absence of Corin (data not shown).

The extent of the yellow band is regulated in part by the limited window in which the agouti gene is expressed in dorsal skin(Millar et al., 1995; Vrieling et al., 1994), and Corin could in principle modulate signals that impinge on DP cells to regulate the expression of agouti. Detailed analysis of agouti expression by real-time PCR during the hair cycle in wild-type mice was performed to define the pattern of agouti expression(Fig. 7B). Agoutitranscript levels were extremely low at P0 but rose rapidly from P1 to P3 and then dropped off dramatically to baseline levels by P7. No changes in the levels or timing of agouti transcript accumulation were detected in Corin mutant mice. This whole skin analysis preferentially detected the expression of agouti in zigzag hairs that comprise 70-80% of the dorsal pelage. Therefore, agouti expression was also evaluated by in situ hybridization to score expression in guard and awl hair follicles(Fig. 7G). As predicted by the lack of a pheomelanin band in Corin mutant guard hairs, agoutiexpression was not detected in guard hair follicles in either wild-type or mutant mice at any of the time points evaluated, which included P0-P6(Fig. 7G and see Fig. S6 in the supplementary material). However, agouti transcripts were readily detected in the DP of some, but not all awl hair follicles at P2 and later stages. No change in the pattern, timing or apparent levels of agoutitranscript accumulation was observed in Corin^-/- skin, and expression in both second and third wave follicles of wild-type and mutant mice was largely extinguished between P6 and P7.

Alternatively, Corin might exert its function by modifying the levels of another component in this signaling pathway downstream of agouti. A decrease in Mc1r expression would be expected to render melanocytes more sensitive to agouti inhibition and expand the `agouti band'. Mc1r mRNA levels in wild-type mice increased to a peak at P3 as the third wave of hair follicles develop and then declined to a stable baseline level of expression that persisted throughout the anagen phase (Fig. 7C). No change in Mc1r expression was detected in the absence of Corin. In addition to directly augmenting Mc1r activity, binding of the agonist α-melanocyte stimulating hormone (α-MSH) to Mc1r competitively inhibits agouti binding(Ollmann et al., 1998). By either mechanism, a decrease in α-MSH levels would be expected to enhance pheomelanin production on an Agouti background. Although Pomc (the gene encoding the precursor of α-MSH) is mainly expressed in the brain, it is also expressed at low levels in the skin and in cultured keratinocytes (Slominski and Paus, 1993). Therefore, Corin may act on the environment surrounding the DP to control mRNA levels of Pomc. However, similar low levels of Pomc expression were detected in the skin of wild-type and Corin mutant mice throughout anagen(Fig. 7D). In addition to Mc1r,the activity of agouti is dependent on at least two other proteins expressed by the melanocyte, attractin (Atrn) and mahogunin (Mgrn1)(Gunn et al., 1999; He et al., 2003a; He et al., 2003b; Phan et al., 2002). Atrn is a single-pass transmembrane protein that binds agouti, and Mgrn1 is an E3 ubiquitin ligase. Although the mechanism by which these proteins modify pigment production remains unknown, the prevailing model is that the simultaneous binding of agouti to Atrn and Mc1r activates Mgrn1 to catalyze ubiquitylation events targeting Mc1r for modifications that decrease its activity, possibly by promoting its degradation or internalization(He et al., 2003a). In contrast to agouti and Mc1r, which are predominantly expressed in the DP and the melanocyte respectively, Atrn and Mgrn1 are more widely expressed. The mRNA levels of Atrn and Mgrn1 were stable throughout the anagen phase of the hair cycle and remained unchanged regardless of Corin genotype(Fig. 7E,F).

Although the lack of Corin did not modify the expression of genes involved in agouti signaling, this analysis does confirm that Corin acts downstream of agouti transcript accumulation to modify pigment production. In wild-type mice, follicles expressing agouti in the DP but nevertheless producing eumelanin in the hair bulb were readily detected at P3 and P5(Fig. 7G and data not shown). By contrast, all follicles expressing agouti in the DP at levels detected by in situ hybridization produce pheomelanin in the hair bulb of Corin mutant mice (Fig. 7G and data not shown). Thus in the absence of Corin, the expression of agouti is sufficient to dictate pheomelanin production,whereas in the presence of Corin, eumelanin production can occur despite agouti mRNA expression.

These results reveal that Corin is quite specifically expressed within the skin in the DP of hair follicles during the anagen phase of the hair cycle. It was not detected elsewhere in the dermis at any other time in development that we analyzed. This specificity, coupled with a dearth of expression elsewhere,other than in cardiomyocytes, makes the Corin locus an attractive platform for strategies to manipulate gene expression in the DP. The fact that a functional Corin gene is not required for the morphogenesis or cycling of the hair follicle extends the range of such strategies to include knock-ins to this locus. The inability to manipulate gene expression in the DP has been an unfortunate impediment to progress in hair follicle biology and the identification of Corin locus as a prime candidate for such approaches is a significant advance.

These results also demonstrate that Corin plays an unexpected role in modifying the balance between eumelanogenesis and pheomelanogenesis. This activity is exerted in concert with the agouti pathway. In the absence of a functional allele of agouti there is no discernible effect on pigment production. It is noteworthy that Corin expression precedes agouti signaling activity and persists long after the decline of detectable agouti activity, whereas the phenotypic effects of Corin ablation are observed at the start and/or the end of agouti signaling period. As Corin expression at the RNA level coincides with protein detection of Corin throughout the anagen phase of the hair cycle (compare Figs 1 and 2), translational regulation does not explain the failure of Corin to suppress pheomelanogenesis during the entire period of agouti expression. Therefore, Corin is only able to effectively counteract agouti activity when the levels of agouti are low, at the peripheries of its bell-shaped curve of expression. This conclusion is supported by the observation that coat color is sensitive to the dosage of agouti in the presence of Corin. The length of the pheomelanin band is reduced when agouti dosage is halved, presumably because a level of agouti sufficient to overcome Corin-mediated inhibition is only reached closer to the peak period of agouti expression. The fact that coat color is also sensitive to the dose of agouti in the absence of Corin further demonstrates that agouti levels are limiting for pheomelanin production at the edges of its period of expression.

The ablation of Corin does not alter the timing or level of agoutiexpression, or the expression of other known components of the agouti signaling pathway. Instead, ablation of Corin unmasks cryptic agouti activity. This cryptic activity is revealed in in situ analysis of agouti expression in wild-type skin, where hair follicles containing both agouti transcripts in the DP and melanocytes producing eumelanin in the adjacent hair bulb are readily detected. By contrast, the deposition of pheomelanin and the expression of agouti transcripts in the DP are well correlated in Corin^-/- skin. In the mutant mice, all DP with detectable agouti expression are embedded in hair bulbs that do not exhibit eumelanin synthesis.

The strong correlation between pheomelanin deposition and the detection of agouti expression in the DP in the absence of Corin suggests that the DP is the source of physiologically relevant agouti in the regulation of pigment production in the dorsal pelage. This observation further suggests that there are no other functionally significant suppressors of agouti protein activity in the mouse strains studied that contribute to this pattern of pigment deposition, although modifiers of pigment production that act at the level of agouti gene expression or independently of agoutimay also contribute to coat color in these strains. A final corollary to this conclusion is that unlike other hair types, agouti expression levels in guard hairs remain below biologically significant levels during the elaboration of hair shafts, which remain black despite the absence of Corin activity. This is substantiated by in situ analysis. Agouti expression was not detected in the DP of guard hair follicles from P0-P7 under conditions that detected agouti transcripts in awl and zigzag follicles (Fig. 7, see Fig. S6 in the supplementary material; data not shown).

These results demonstrate that Corin acts downstream of agoutigene transcript accumulation to functionally counteract agouti protein activity. As a protease tethered to the surface of the DP cell, the Corin protein is well positioned to regulate agouti protein activity in several ways. One known Corin substrate is the prohormone Nppa, which when cleaved to its active form, contributes to blood pressure regulation by activating the natriuretic peptide pathway (Chan et al.,2005; Wu et al.,2002; Yan et al.,1999; Yan et al.,2000). Although we can detect the expression of the Nppband Nppc genes that encode related natriuretic peptide precursors in the skin at low levels by PCR, neither these genes nor the signaling receptors Npr1 and Npr2 are detectable in the hair follicle by in situ hybridization under conditions that readily detect the robust expression of Nppaand Nppb in the heart (data not shown). Although we cannot rule out the Npp family as substrates that mediate this effect, alternative mechanisms seem more likely.

Among these, a direct protein-protein interaction with a component of the agouti pathway is a strong possibility. As secreted or transmembrane proteins,agouti, α-MSH (Pomc1), Atrn and Mc1r are all potential targets whose activity could be attenuated (agouti or Atrn) or augmented (Mc1r orα-MSH) by the extracellular Corin protease activity. Of these,α-MSH is unlikely to be the target of Corin. Although Pomc1-null mice have lighter coat color, particularly in the ventral skin, they do not resemble the dramatic effect on coat color observed in Corin-deficient mice(Challis et al., 2004; Yaswen et al., 1999). By contrast, the agouti protein is clearly limiting for the pheomelanin switch in wild-type animals (Miller et al.,1997). The fact that it is secreted from the DP, where Corin levels are high, also makes agouti an attractive candidate for modification by this protease.

The fact that the N-terminal and C-terminal domains of agouti bind separately and independently to Atrn and Mc1r, respectively, and the failure of these two domains to rescue in trans the activity of full-length agouti(He et al., 2001; Ollmann and Barsh, 1999),suggest that binding of agouti to both Atrn and Mc1r forms a ternary complex that is required for agouti signaling. If so, a proteolytic cleavage that physically separates the N-terminal domain from the C-terminal domain would not only render the agouti protein inactive but could also produce competitive antagonists that interfere with agouti binding to both Mc1r and Atrn.

Agouti binding to Mc1r is conformation dependent. Cis and trans conformers with markedly different abilities to compete for Mc1r binding are generated when the C-terminal domain of agouti is synthesized in vitro(McNulty et al., 2005). The fact that agouti protein can assume different conformations with distinct binding capabilities suggests that activity of agouti protein could also be modified by a proteolytic cleavage that promotes a conformational change that reduces or abolishes the affinity of agouti protein for either Mc1r, Atrn or both.

In ventral skin, where agouti levels remain high and promote pheomelanin production throughout the hair cycle, the majority of agouti protein detected by western analysis migrates at the position expected of the full-length form(Ollmann and Barsh, 1999). Unfortunately, the low and variable level of agouti protein in dorsal skin,the limited sensitivity of available reagents, and the partial nature of the predicted cleavage events prevent direct assessment of these potential cleavage models in dorsal skin in vivo.

Although mechanisms in which Corin acts directly on agouti might limit the impact of these observations on human pigmentation, where a postulated role for agouti in pigmentation based on linkage analysis remains controversial, they might still have other implications for human health. Ectopic expression of agouti in the brain induces obesity by acting on the Mc4r that is normally inhibited by the agouti-related protein (Agrp)(Duhl et al., 1994; Manne et al., 1995). Aspects of these two signaling pathways are distinct. First, agouti activity in the brain is dependent on attractin and mahogunin(He et al., 2001) whereas Agrp activity is not (He et al.,2003a; Ollmann et al.,1997). Second, although the C- and N-terminal domains of agouti must act in concert to inhibit Mc1r activity in vivo, binding of the C-terminal domain of Agrp to its receptor is sufficient for function(Ollmann et al., 1997). Furthermore, the N-terminal domain of Agrp inhibits this function and must be removed by proteolytic cleavage to activate Agrp(Creemers et al., 2006; Jackson et al., 2006). Thus,cleavage between the N- and C-terminal domains is likely to have opposite effects on Agouti and Agrp activity, but other possible proteolytic modifications may have similar effects on both pathways. Although Corin is not expressed in the CNS(Yan et al., 1999), it is possible that an analogous serine protease modulates the activity of Agrp signaling and thus participates in the process of weight control and energy balance. Whether Corin acts by modifying some component of agouti signaling or by a natriuretic peptide pathway-related mechanism that antagonizes Mc1r responses in melanocytes, these results identify a novel pathway to modulate Mcr activity. Identifying the postulated analogous serine protease in the brain would increase the repertoire of targets for drug development and allow the exploration of new therapeutics against obesity.

Finally, variations in coat color have played a prominent role in discussions of adaptation to the environment and the mechanisms that drive evolution. Although a large number of genes contribute to pigmentation either through functions in the generation, migration or maintenance of melanocytes,biosynthesis of eumelanin, or transfer of pigment to recipient cells,comparatively few have been identified that contribute to the pheomelanin/eumelanin switch that plays such a significant role in coat appearance(http://www.cbc.umn.edu/ifpcs/micemut.htm). We note that the Corin mutation reproduces the described differences in agouti band length seen in deer mouse populations adapted to lighter colored environments than their forest-dwelling counterparts(Hoekstra, 2006). The observations that levels of agouti signaling are limiting in the switch to pheomelanin production and are effectively regulated by Corin only when agouti activity is marginal identifies a novel route to the phenotypic changes associated with adaptive coloration changes. Loss-of-function mutations in Pomc cause a similar albeit less dramatic lightening of the dorsal pelage, but also cause obesity and adrenal insufficiency(Krude et al., 1998; Yaswen et al., 1999). Loss of Corin function may also incur other fitness costs, as both modest hypertension and cardiac hypertrophy are observed in mutant mice, but these costs are less dramatic (Chan et al.,2005). Furthermore, if pigmentation and cardiovascular regulation use different substrates, these two processes can be uncoupled by substrate-specificity mutations (Knappe et al., 2004). These attributes, and the lack of other genetic modifiers downstream of agouti expression, suggest that an analysis of the relative contribution of Corin mutations to adaptive changes in pigmentation in wild populations will be informative.

In conclusion, these studies identify the transmembrane serine protease Corin as a novel modifier of the agouti signaling pathway that acts downstream of agouti gene expression to suppress agouti protein activity on the pheomelanin/eumelanin switch. The tight restriction of Corinexpression to the DP during the anagen phase of the hair cycle within the skin, its relative lack of expression elsewhere and the apparent restriction of its activity to the regulation of pigment patterning, all suggest that this gene will serve as a useful platform to manipulate gene expression in the DP. This will broaden its contribution to the understanding of hair follicle biology beyond these important insights into the regulation of coat color.

We thank Ying Zheng for genotyping and technical assistance, Hidenao Shimizu for discussions of this work and unpublished data, and the MGH ES core for assistance in generating the mutant mice. This work was supported by a grant to the Cutaneous Biology Research Center from Shiseido, Ltd. and to B.A.M. from the National Institutes of Health (R01AR055256-01).