In higher plants, molecular mechanisms regulating shoot apical meristem (SAM) formation and organ separation are largely unknown. The CUC1 (CUP-SHAPED COTYLEDON1) and CUC2 are functionally redundant genes that are involved in these processes. We cloned the CUC1 gene by a map-based approach, and found that it encodes a NAC-domain protein highly homologous to CUC2. CUC1 mRNA was detected in the presumptive SAM during embryogenesis, and at the boundaries between floral organ primordia. Surprisingly, overexpression of CUC1 was sufficient to induce adventitious shoots on the adaxial surface of cotyledons. Expression analyses in the overexpressor and in loss-of-function mutants suggest that CUC1 acts upstream of the SHOOT MERISTEMLESS gene.
Reference
Aida
M.
, Ishida
T.
, Fukaki
H.
, Fujisawa
H.
, Tasaka
M.
(1997
) Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.
Plant Cell
9
, 841
–857
Aida
M.
, Ishida
T.
, Tasaka
M.
(1999
) Shoot apical meristem and cotyledon formation during Arabidopsis embryogenesis: interaction among the CUP-SHAPED COTYLEDON and SHOOT MERISTEMLESS genes.
Development
126
, 1563
–1570
An
Y.-Q.
, McDowell
J. M.
, Huang
S.
, McKinney
E. C.
, Chambliss
S.
, Meagher
R. B.
(1996
) Strong, constitutive expression of the Arabidopsis ACT2 / ACT8 actin subclass in vegetative tissues.
Plant J
10
, 107
–121
Barton
M. K.
, Poethig
R. S.
(1993
) Formation of the shoot apical meristem in Arabidopsis thaliana: an analysis of development in the wild type and in the shoot meristemless mutant.
Development
119
, 823
–831
Chuck
G.
, Lincoln
C.
, Hake
S.
(1996
) KNAT1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis.
Plant Cell
8
, 1277
–1289
Endrizzi
K.
, Moussian
B.
, Haecker
A.
, Levin
J. Z.
, Laux
T.
(1996
) The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE.
Plant J
10
, 967
–979
Estruch
J. J.
, Prinsen
E.
, Onckelen
H. V.
, Schell
J.
, Spena
A.
(1991
) Viviparous leaves produced by somatic activation of an inactive cytokinin-synthesizing gene.
Science
254
, 1364
–1367
Ishida
T.
, Aida
M.
, Takada
S.
, Tasaka
M.
(2000
) Involvement of CUP-SHAPED COTYLEDON genes in gynoecium and ovule development in Arabidopsis thaliana.
Plant Cell Physiol
41
, 60
–67
Jackson
D.
, Veit
B.
, Hake
S.
(1994
) Expression of maize KNOTTED-1 related homeobox genes in the shoot apical meristem predicts patterns of morphogenesis in the vegetative shoot.
Development
120
, 405
–413
Jefferson
R. A.
, Kavanagh
T. A.
, Bevan
M. V.
(1987
) GUS fusions:-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
EMBO J
6
, 3901
–3907
John
I.
, Hackett
R.
, Cooper
W.
, Drake
R.
, Farrell
A.
, Grierson
D.
(1997
) Cloning and characterization of tomato leaf senescence-related cDNAs.
Plant Mol. Biol
33
, 641
–651
Laux
T.
, Mayer
K. F. X.
, Berger
J.
, Jurgens
G.
(1996
) The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis.
Development
122
, 87
–96
Lincoln
C.
, Long
J.
, Yamaguchi
J.
, Serikawa
K.
, Hake
S.
(1994
) A knotted1 -like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants.
Plant Cell
6
, 1859
–1876
Long
J. A.
, Moan
E. I.
, Medford
J. I.
, Barton
M. K.
(1996
) A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis.
Nature
379
, 66
–69
Long
J. A.
, Barton
M. K.
(1998
) The development of apical embryonic pattern in Arabidopsis.
Development
125
, 3027
–3035
Lynn
K.
, Fernandez
A.
, Aida
M.
, Sedbrook
J.
, Tasaka
M.
, Masson
P.
, Barton
M. K.
(1999
) The PINHEAD / ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene.
Development
126
, 469
–481
Mayer
K. F. X.
, Schoof
H.
, Haecker
A.
, Lenhard
M.
, Jurgens
G.
, Laux
T.
(1998
) Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem.
Cell
95
, 805
–815
McConnell
J. R.
, Barton
M. K.
(1995
) Effect of mutations in the PINHEAD gene of Arabidopsis on the formation of shoot apical meristems.
Dev. Genet
16
, 358
–366
McConnell
J. R.
, Barton
M. K.
(1998
) Leaf polarity and meristem formation in Arabidopsis.
Development
125
, 2935
–2942
Michener
C. D.
, Sokal
R. R.
(1957
) A quantitative approach to a problem in classification.
Evolution
11
, 130
–162
Moussian
B.
, Schoof
H.
, Haecker
A.
, Jurgens
G.
, Laux
T.
(1998
) Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis.
EMBO J
17
, 1799
–1809
Neff
M. M.
, Neff
J. D.
, Chory
J.
, Pepper
A. E.
(1998
) dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics.
Plant J
14
, 387
–392
Ruiz-Medrano
R.
, Xoconostle-Cázares
B.
, Lucas
W. J.
(1999
) Phloem long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants.
Development
126
, 4405
–4419
Sawa
S.
, Watanabe
K.
, Goto
K.
, Kanaya
E.
, Morita
E. H.
, Okada
K.
(1999
) FILAMENTOUS FLOWER, a meristem and organ identity gene of Arabidopsis, encodes a protein with a zinc finger and HMG-related domains.
Genes Dev
13
, 1079
–1088
Schneider
M.
, Ow
D. W.
, Howell
S. H.
(1990
) The in vivo pattern of firefly luciferase expression in transgenic plants.
Plant Mol. Biol
14
, 935
–947
Siegfried
K. R.
, Eshed
Y.
, Baum
S. F.
, Otsuga
D.
, Drews
G. N.
, Bowman
J. L.
(1999
) Members of the YABBY gene family specify abaxial cell fate in Arabidopsis.
Development
126
, 4117
–4128
Sinha
N. R.
, Williams
R. E.
, Hake
S.
(1993
) Overexpression of the maize homeo box gene, KNOTTED-1, causes a switch from determinate to indeterminate cell fates.
Genes Dev
7
, 787
–795
Smith
L. G.
, Jackson
D.
, Hake
S.
(1995
) Expression of knotted1 marks shoot meristem formation during maize embryogenesis.
Dev. Genet
16
, 344
–348
Smyth
D. R.
, Bowman
J. L.
, Meyerowitz
E. M.
(1990
) Early flower development in Arabidopsis.
Plant Cell
2
, 755
–767
Souer
E.
, Van Houwelingen
A.
, Kloos
D.
, Mol
J.
, Koes
R.
(1996
) The no apical meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries.
Cell
85
, 159
–170
Waites
R.
, Hudson
A.
(1995
) phantastica: a gene required for dorsoventrality of leaves in Antirrhinum majus.
Development
121
, 2143
–2154
Waites
R.
, Selvadurai
H. R. N.
, Oliver
I. R.
, Hudson
A.
(1998
) The PHANTASTICA gene encodes a MYB transcription factor involved in growth and dorsoventrality of lateral organs in Antirrhinum.
Cell
93
, 779
–789
West
M. A. L.
, Harada
J. J.
(1993
) Embryogenesis in higher plants: an overview.
Plant Cell
5
, 1361
–1369
Xie
Q.
, Sanz-Burgos
A. P.
, Guo
H.
, García
J. A.
, Gutierrez
C.
(1999
) GRAB proteins, novel members of the NAC domain family, isolated by their interaction with a geminivirus protein.
Plant Mol. Biol
39
, 647
–656
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