In ventricular cells of the mouse telencephalon, differential expression of cadherin cell adhesion molecules defines neighbouring regions; R-cadherin delineates the future cerebral cortex, while cadherin-6 delineates the lateral ganglionic eminence. By using cell labelling analyses in the whole embryo culture system, we demonstrated that the interface between R-cadherin and cadherin-6 expression is a boundary for cell lineage restriction at embryonic day 10.5. Interestingly, when a group of cells with exogenous cadherin-6 were generated to straddle the cortico-straital boundary by electroporation at embryonic day 11.0, ectopic cadherin-6-expressing cortical cells were sorted into the striatal compartment, and the reverse was the trend for ectopic R-cadherin-expressing striatal cells. Although cadherin-6 gene knockout mice engineered in this study showed no obvious phenotype in telencephalic compartmentalisation, the preferential sorting of ectopic cadherin-6-expressing cells was abolished in this mutant background. Thus, the differential expression pattern of cadherins in the embryonic telencephalon is responsible for maintaining the cortico-striatal compartment boundary.

Akamatsu
W.
,
Okano
H. J.
,
Osumi
N.
,
Inoue
T.
,
Nakamura
S.
,
Sakakibara
S.
,
Miura
M.
,
Matsuo
N.
,
Darnell
R. B.
,
Okano
H.
(
1999
)
Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous system.
Proc. Natl. Acad. Sci. USA
96
,
9885
9890
Anderson
S. A.
,
Eisenstat
D. D.
,
Shi
L.
,
Rubenstein
J. L.
(
1997
)
Interneuron migration from basal forebrain to neocortex: dependence on Dlx genes.
Science
278
,
474
476
Chapouton
P.
,
Gärtner
A.
,
Götz
M.
(
1999
)
The role of Pax6 in restricting cell migration between developing cortex and basal ganglia.
Development
126
,
5569
5579
Dahmann
C.
,
Basler
K.
(
1999
)
Compartment boundaries at the edge of development.
Trends Genet
15
,
320
326
Dahmann
C.
,
Basler
K.
(
2000
)
Opposing transcriptional outputs of hedgehog signaling and engrailed control compartmental cell sorting at the Drosophila A/P boundary.
Cell
100
,
411
422
Espeseth
A.
,
Marnellos
G.
,
Kintner
C.
(
1998
)
The role of F-cadherin in localizing cells during neural tube formation in Xenopus embryos.
Development
125
,
301
312
Figdor
M. C.
,
Stern
C. D.
(
1993
)
Segmental organization of embryonic diencephalon.
Nature
363
,
630
634
Fishell
G.
,
Mason
C.A.
,
Hatten
M.E.
(
1993
)
Dispersion of neural progenitors within the germinal zones of the forebrain.
Nature
362
,
636
638
Fraser
S.
,
Keynes
R.
,
Lumsden
A.
(
1990
)
Segmentation in the chick embryo hindbrain is defined by cell lineage restrictions.
Nature
344
,
431
435
Fujimori
T.
,
Takeichi
M.
(
1993
)
Disruption of epithelial cell-cell adhesion by exogenous expression of a mutated nonfunctional N-cadherin.
Mol. Biol. Cell
4
,
37
47
Götz
M.
,
Wizenmann
A.
,
Reinhardt
S.
,
Lumsden
A.
,
Price
J.
(
1996
)
Selective adhesion of cells from different telencephalic regions.
Neuron
16
,
551
564
Garcia-Bellido
A.
(
1966
)
Changes in selective affinity following transdetermination in imaginal disc cells of Drosophila melanogaster.
Exp. Cell Res
44
,
382
392
Inoue
T.
,
Chisaka
O.
,
Matsunami
H.
,
Takeichi
M.
(
1997
)
Cadherin-6 expression transiently delineates specific rhombomeres, other neural tube subdivisions, and neural crest subpopulations in mouse embryo.
Dev. Biol
183
,
183
194
Inoue
T.
,
Tanaka
T.
,
Suzuki
S. C.
,
Takeichi
M.
(
1998
)
Cadherin-6 in the developing brain: Expression along restricted connection systems and synaptic localisation suggest a potential role in neuronal circuitry.
Dev. Dyn
211
,
338
351
Inoue
T.
,
Nakamura
S.
,
Osumi
N.
(
2000
)
Fate mapping of the mouse prosencephalic neural plate.
Dev. Biol
219
,
373
383
Lumsden
A.
(
1990
)
The cellular basis of segmentation in the developing hindbrain.
Trends. Neurosci
13
,
329
335
Lumsden
A.
,
Krumlauf
R.
(
1996
)
Patterning the vertebrate neuroaxis.
Science
274
,
1109
1115
Matsunami
H.
,
Miyatani
S.
,
Inoue
T.
,
Copeland
N. G.
,
Gilbert
D. J.
,
Jenkins
N. A.
,
Takeichi
M.
(
1993
)
Cell binding specificity of mouseR-cadherin and chromosomal mapping of the gene.
J. Cell Sci
106
,
401
409
Matsunami
H.
,
Takeichi
M.
(
1995
)
Fetal brain subdivisions defined by R-and E-cadherin expressions: evidence for the role of cadherin activity in region-specific, cell-cell adhesion.
Dev. Biol
172
,
466
478
Mellizer
G.
,
Xu
Q.
,
Wilkinson
D. G.
(
1999
)
Eph receptors and ephrinns restrict cell intermingling and communication.
Nature
400
,
77
81
Miyatani
S.
,
Shimamura
K.
,
Hatta
M.
,
Nagafuchi
A.
,
Nose
A.
,
Matsunaga
M.
,
Hatta
K.
,
Takeichi
M.
(
1989
)
Neural cadherin: role in selective cell-cell adhesion.
Science
245
,
631
635
Muramatsu
T.
,
Mizutani
Y.
,
Ohmori
Y.
,
Okumura
J.
(
1997
)
Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo.
Biochem. Biophys. Res. Commun
230
,
376
380
Nagafuchi
A.
,
Shirayoshi
Y.
,
Okazaki
K.
,
Yasuda
K.
,
Takeichi
M.
(
1987
)
Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA.
Nature
329
,
341
343
Nose
A.
,
Nagafuchi
A.
,
Takeichi
M.
(
1988
)
Expressed recombinant cadherins mediate cell sorting in model systems.
Cell
54
,
993
1001
Osumi-Yamashita
N.
,
Ninomiya
Y.
,
Eto
K.
(
1997
)
Mammalian craniofacial embryology in vitro.
Int. J. Dev. Biol
41
,
187
194
Pules
L.
,
Rubenstein
J. L. R.
(
1993
)
Expression pattern of homeoboxand other putative regulatory genes in the embryonic mouse forebrain suggest a neuromeric organization.
Trends. Neurosci
16
,
472
479
Redies
C.
,
Takeichi
M.
(
1996
)
Cadherins in the developing central nervous system: an adhesive code for segmental and functional subdivisions.
Dev. Biol
180
,
413
423
Redies
C.
(
2000
)
Cadherins in the central nervous system.
Prog. Neurobiol
61
,
611
648
Rhinn
M.
,
Dierich
A.
,
LeMeur
M.
,
Ang
S. L.
(
1999
)
Cell autonomous and non-cell autonomous functions of Otx2 in patterning the rostral brain.
Development
126
,
4295
4304
Rubenstein
J. L. R.
,
Martinez
S.
,
Shimamura
K.
,
Puelles
L.
(
1994
)
The embryonic vertebrate forebrain: the prosomeric model.
Science
266
,
578
580
Steinberg
M. S.
,
Takeichi
M.
(
1994
)
Experimental specification of cell sorting, tissue spreading, and spatial patterning by quantitative differences in cadherin expression.
Proc. Natl. Acad. Sci. USA
91
,
206
209
Stoykova
A.
,
Götz
M.
,
Gruss
P.
,
Price
J.
(
1997
)
Pax6-dependent regulation of adhesive patterning, R-cadherin expression and boundary formation in developing forebrain.
Development
124
,
3765
3777
Swiatek
P. J.
,
Grindley
T.
(
1993
)
Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox20.
Genes Dev
7
,
2071
2084
Takeichi
M.
(
1991
)
Cadherin cell adhesion receptors as a morphogenetic regulator.
Science
251
,
1451
1455
Takeichi
M.
(
1995
)
Morphogenetic roles of classic cadherins.
Curr. Opin. Cell Biol
7
,
619
627
Tamamaki
N.
,
Fujimori
K. E.
,
Takauji
R.
(
1997
)
Origin and route of tangentially migrating neurons in the developing neocortical intermediate zone.
J. Neurosci
17
,
8313
8323
Townes
P. L.
,
Holtfreter
J.
(
1955
)
Directed movements and selective adhesion of embryonic amphibian cells.
J. Exp. Zool
128
,
53
120
Wizenmann
A.
,
Lumsden
A.
(
1997
)
Segregation of rhombomeres by differential chemoaffinity.
Mol. Cell. Neurosci
9
,
448
459
Xu
Q.
,
Mellitzer
G.
,
Robinson
V.
,
Wilkinson
D. G.
(
1999
)
In vivo cell sorting in complementary segmental domains mediated by Eph receptors and ephrins.
Nature
399
,
267
271
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