During early Drosophila oogenesis, one cell from a cyst of 16 germ cells is selected to become the oocyte, and accumulates oocyte-specific proteins and the centrosomes from the other 15 cells. Here we show that the microtubule cytoskeleton and the centrosomes follow the same stepwise restriction to one cell as other oocyte markers. Surprisingly, the centrosomes still localise to one cell after colcemid treatment, and in BicD and egl mutants, which abolish the localisation of all other oocyte markers and the polarisation of the microtubule cytoskeleton. In contrast, the centrosomes fail to migrate in cysts mutant for Dynein heavy chain 64C, which disrupts the fusome. Thus, centrosome migration is independent of the organisation of the microtubule cytoskeleton, and seems to depend instead on the polarity of the fusome.

Byers
T.
,
Dubreuil
R.
,
Branton
D.
,
Kiehart
D.
,
Goldstein
L.
(
1987
)
Drosophila spectrin II: Conserved features of the alpha-subunit are revealed by analysis of cDNA clones and fusion proteins.
J. Cell Biol
105
,
2103
2110
Carpenter
A.
(
1975
)
Electron microscopy of meiosis in Drosophila melanogaster females. I. Structure, arrangement, and temporal change of the synaptonemal complex in wild-type.
Chromosoma
51
,
157
182
Chou
T.-B.
(
1992
)
Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.
Genetics
131
,
643
653
Clark
I.
,
Jan
L.
,
Jan
Y.
(
1997
)
Reciprocal localization of Nod and kinesin fusion proteins indicates microtubule polarity in the Drosophila oocyte, epithelium, neuron and muscle.
Development
124
,
461
470
de Cuevas
M.
,
Spradling
A.
(
1998
)
Morphogenesis of the Drosophila fusome and its implications for oocyte specification.
Development
125
,
2781
2799
Deng
W.
,
Lin
H.
(
1997
)
Spectrosomes and fusomes anchor mitotic spindles during asymmetric germ cell divisions and facilitate the formation of a polarized microtubule array for oocyte specification in Drosophila.
Dev. Biol
189
,
79
94
Field
C.
,
Alberts
B.
(
1984
)
Anillin, a contractile ring protein that cycles from the nucleus to the cortex.
J. Cell Biol
131
,
165
178
Gegerly
F.
,
Kidd
D.
,
Jeffers
K.
,
Wakefield
J.
,
Raff
J.
(
2000
)
D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo.
EMBO J
19
,
241
252
González
C.
,
Tavosanis
G.
,
Mollinari
C.
(
1998
)
Centrosomes and microtubule organisation during Drosophila development.
J. Cell Sci
111
,
2697
2706
González-Reyes
A.
,
St Johnston
D.
(
1998
)
The Drosophila AP axis is polarised by the cadherin-mediated positioning of the oocyte.
Development
125
,
3635
3644
Grieder
N.
,
de Cuevas
M.
,
Spradling
A.
(
2000
)
The fusome organizes the microtubule network during oocyte differentiation in Drosophila.
Development
127
,
4253
4264
Hirokawa
N.
(
1998
)
Kinesin and dynein superfamily proteins and the mechanism of organelle transport.
Science
279
,
519
524
Huettner
A.
(
1933
)
Continuity of the centrioles in Drosophilamelanogaster.
Z. Zellforsch. Mikroskop. Anat
19
,
119
134
Huynh
J.-R.
,
St Johnston
D.
(
2000
)
The role of BicD, Egl, Orb and the microtubules in the restriction of meiosis to the Drosophila oocyte.
Development
127
,
2785
2794
Joshi
H.
(
1994
)
Microtubule organizing centers and-tubulin.
Curr. Opin. Cell Biol
6
,
55
62
Keyes
L.
,
Spradling
A.
(
1997
)
The Drosophila gene fs(2)cup interacts with otu to define a cytoplasmic pathway required for the structure and function of germ-line chromosomes.
Development
124
,
1419
1431
Koch
E.
,
Spitzer
R.
(
1983
)
Multiple effects of colchicine on oogenesis in Drosophila; Induced sterility and switch of potencial oocyte to nurse-cell developmental pathway.
Cell Tissue Res
228
,
21
32
Kraut
R.
,
Chia
W.
,
Jan
L.
,
Jan
Y.
,
Knoblich
J.
(
1996
)
Role of inscuteable in orienting asymmetric cell divisions in Drosophila.
Nature
383
,
50
55
Lantz
V.
,
Chang
J.
,
Horabin
J.
,
Bopp
D.
,
Schedl
P.
(
1994
)
The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity.
Genes Dev
8
,
598
613
Li
K.
,
Kaufman
T.
(
1996
)
The homeotic target gene centrosomin encodes an essential centrosomal component.
Cell
85
,
585
596
Li
M.
,
McGrail
M.
,
Serr
M.
,
Hays
T.
(
1994
)
Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte.
J. Cell Biol
126
,
1475
1494
Lin
H.
,
Spradling
A.
(
1995
)
Fusome asymmetry and oocyte determination in Drosophila.
Dev. Genet
16
,
6
12
Mach
J.
(
1997
)
An egalitarian-BicaudalD complex is essential for oocyte specification and axis determination in Drosophila.
Genes Dev
11
,
423
435
Mahowald
A. P.
,
Strassheim
J. M.
(
1970
)
Intercellular migration of centrioles in the germarium of Drosophila melanogaster.
J. Cell Biol
45
,
306
320
McGrail
M.
,
Hays
T.
(
1997
)
The microtubule motor cytoplasmic dynein is required for spindle orientation during germline divisions and oocyte differentiation in Drosophila.
Development
124
,
2409
2419
McKearin
D.
(
1997
)
The Drosophila fusome, organelle biogenesis and germ cell differentiation: if you build it.
BioEssays
19
,
147
152
Mogensen
M.
,
Tucker
J.
,
Stebbings
H.
(
1989
)
Microtubule polarities indicate that nucleation and capture of microtubules occurs at cell surfaces in Drosophila.
J. Cell Biol
108
,
1445
1452
Ran
B.
,
Bopp
R.
,
Suter
B.
(
1994
)
Null alleles reveal novel requirements for Bic-D during Drosophila oogenesis and zygotic development.
Development
120
,
1233
1242
Rorth
P.
(
1998
)
Gal4 in the Drosophila female germline.
Mech. Dev
78
,
113
118
Schupbach
T.
,
Wieschaus
E.
(
1991
)
Female sterile mutations on the second chromosome of Drosophilamelanogaster. II. Mutations blocking oogenesis or altering egg morphology.
Genetics
129
,
1119
1136
Stearns
T.
,
Winey
M.
(
1997
)
The cell center at 100.
Cell
91
,
303
309
Suter
B.
,
Romberg
L.
,
Steward
R.
(
1989
)
Bicaudal-D, a Drosophila gene involved in developmental asymmetry: localized transcript accumulation in ovaries and sequence similarity to myosin heavy chain tail domains.
Genes and Dev
3
,
1957
1968
Suter
B.
,
Steward
R.
(
1991
)
Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
Cell
67
,
917
926
Theurkauf
W.
(
1994
)
Microtubules and cytoplasm organization during Drosophila oogenesis.
Dev. Biol
165
,
352
360
Theurkauf
W.
,
Alberts
M.
,
Jan
Y.
,
Jongens
T.
(
1993
)
A central role for microtubules in the differentiation of Drosophila oocytes.
Development
118
,
1169
1180
Theurkauf
W.
,
Smiley
S.
,
Wong
M.
,
Alberts
B.
(
1992
)
Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport.
Development
115
,
923
936
Tucker
J.
,
Milner
M.
,
Currie
D.
,
Muir
J.
,
Forrest
D.
,
Spencer
M.
(
1986
)
Centrosomal microtubule-organizing centres and a switch in the control for protofilament number of cell surface-associated microtubules during Drosophila wing morphogenesis.
Eur. J. Cell. Biol
41
,
279
289
Van Doren
M.
,
Williamson
A.
,
Lehmann
R.
(
1998
)
Regulation of zygotic genen expression in Drosophila primordial germ cells.
Curr. Biol
8
,
243
246
Wharton
R. P.
,
Struhl
G.
(
1989
)
Structure of the DrosophilaBicaudal-D protein and its role in localizing the posterior determinant nanos.
Cell
59
,
881
892
Zhang
P.
,
Knowles
B.
,
Goldstein
L.
,
Hawley
R.
(
1990
)
A kinesin-like protein required for distributive chromosome segregation in Drosophila.
Cell
62
,
1053
1062
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