Clim-2 (NLI, Lbd1) is one of two related mouse proteins that interact with Lim-domain homeoproteins. In the mouse, embryonic expression of Clim-2 is particularly pronounced in facial ectomesenchyme and limb bud mesenchyme in association with Lim genes, Lhx-6 and Lmx-1 respectively. We show that in common with both these Lim genes, Clim-2 expression is regulated by signals from overlying epithelium. In both the developing face and the limb buds we identify Fgf-8 as the likely candidate signalling molecule that regulates Clim-2 expression. We show that in the mandibular arch, as in the limb, Fgf-8 functions in combination with CD44, a cell surface binding protein, and that blocking CD44 binding results in inhibition of Fgf8-induced expression of Clim-2 and Lhx-6. Regulation of gene expression by Fgf8 in association with CD44 is thus conserved between limb and mandibular arch development.
Conserved regulation of mesenchymal gene expression by Fgf-8 in face and limb development
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A.S. Tucker, A. Al Khamis, C.A. Ferguson, I. Bach, M.G. Rosenfeld, P.T. Sharpe; Conserved regulation of mesenchymal gene expression by Fgf-8 in face and limb development. Development 15 January 1999; 126 (2): 221–228. doi: https://doi.org/10.1242/dev.126.2.221
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