A MYB-related transcription factor (MIXTA) that controls development of conical cell form is expressed only in the inner epidermis of Antirrhinum petals. Expression of this gene throughout transgenic tobacco plants leads to excess numbers of multicellular trichomes on leaves and floral organs as well as the novel production of conical cells on leaves. These data indicate that conical cells and trichomes are produced by a common developmental pathway. The timing of MIXTA expression suggests that the choice between the cell types depends on the competence for cell division at the time at which the controlling gene is expressed. Duplication of genes and their association with different cis-regulatory regions may therefore result in the specification of novel plant cell types.
REFERENCES
Akers
C. P.
, Weybrew
J. A.
, Long
R. C.
(1978
). Ultrastructure of glandular trichomes of leaves of Nicotiana tabacum L., cv Xanthi.
Am. J. Bot
65
, 282
–292
Avila
J.
, Nieto
C.
, Canas
L.
, Benito
M. J.
, Paz-Ares
J.
(1993
) Petunia hybrida genes related to the maize regulatory C1 gene and to animal myb proto-oncogenes.
Plant J
3
, 553
–562
Beals
T. P.
, Goldberg
R. B.
(1997
) A novel cell ablation strategy blocks tobacco anther dehiscence.
Plant Cell
9
, 1527
–1545
Bevan
M.
(1984
) Binary Agrobacterium vectors for plant transformation.
Nucl. Acids Res
12
, 8711
–8721
Bradley
D.
, Carpenter
R.
, Sommer
H.
, Hartley
N.
, Coen
E.
(1993
) Complementary floral homeotic phenotypes result from opposite orientations of a transposon at the plena locus of Antirrhinum.
Cell
72
, 85
–95
Chien
J. C.
, Sussex
I. M.
(1996
). Differential regulation of trichome formation on the adaxial and abaxial leaf surfaces by gibberellins and photoperiod in Arabidopsis thaliana (L.) Heynh.
Plant Physiol
111
, 1321
–1328
Croxdale
J.
, Smith
J.
, Yandell
B.
, Johnson
B.
(1992
) Stomatal patterning in Tradescantia: An evaluation of the cell lineage theory.
Dev. Biol
149
, 158
–167
Fahn
A.
(1985
) Structural and functional properties of trichomes of xeromorphic leaves.
Ann. Bot
57
, 631
–637
Forbert
P. R.
, Gaudin
V.
, Lunness
P.
, Coen
E. S.
, Doonan
J. H.
(1996
) Distinct classes of cdc2-related genes are differentially expressed during the cell-division cycle in plants.
Plant Cell
8
, 1465
–1476
Goodrich
J.
, Carpenter
R.
, Coen
E. S.
(1992
) A common gene regulates pigmentation pattern in diverse plant species.
Cell
68
, 955
–964
Goodrich
J.
, Puangsomiee
P.
, Martin
M.
, Long
D.
, Meyerowitz
E.
, Coupland
G.
(1997
) A polycomb-group gene regulates homeotic gene expression in Arabidopsis.
Nature,
386
, 44
–51
Goff
S. A.
, Cone
K. C.
, Chandler
V. L.
(1992
) Functional analysis of the transcriptional activator encoded by the maize B gene: evidence for a direct functional interaction between two classes of regulatory proteins.
Genes Dev
6
, 864
–875
Goldberg
R.B.
(1988
) Plants: novel developmental processes.
Science
240
, 1460
–1467
Gorton
H. L.
, Vogelmann
T. C.
(1996
) Effects of epidermal cell shape and pigmentation on optical properties of Antirrhinum petals at visible and ultraviolet wavelengths.
Plant Physiol
112
, 879
–888
Horsch
R. B.
, Fry
J. E.
, Hoffmann
N. L.
, Eichholtz
D.
, Rogers
S. G.
, Fraley
R. T.
(1985
) A simple and general method for transferring genes into plants.
Science
227
, 1229
–1231
Hulskamp
M.
, Misera
S.
, Jurgens
G.
(1994
) Genetic dissection of trichome cell development in Arabidopsis.
Cell
76
, 555
–566
Jackson
D.
, Culianez-Macia
F.
, Prescott
A. G.
, Roberts
K.
, Martin
C.
(1991
) Expression patterns of Myb genes from Antirrhinum flowers.
Plant Cell,
3
, 115
–125
Kevan
P. G.
, Lane
M. A.
(1985
) Flower petal microtexture is a tactile cue for bees.
Proc. Natl. Acad. Sci. USA
82
, 4750
–4752
Larkin
J. C.
, Oppenheimer
D. G.
, Lloyd
A. M.
, Paparozzi
E. T.
, Marks
M. D.
(1994
) Roles of the GLABROUS1 and TRANSPARENT TESTA GLABROUS genes in Arabidopsis trichome development.
Plant Cell
6
, 1065
–1076
Larkin
J. C.
, Marks
M. D.
, Nadeau
J.
, Sack
F.
(1997
) Epidermal cell fate and patterning in leaves.
Plant Cell
9
, 1109
–1120
Larkin
J. C.
, Young
N.
, Prigge
M.
, Marks
M. D.
(1996
) The control of trichome spacing and number in Arabidopsis.
Development
122
, 997
–1005
Li
X.
, Noll
M.
(1994
) Evolution of distinct developmental functions of three Drosophila genes by acquisition of different cis-regulatory regions.
Nature
367
, 83
–87
Lloyd
A.M.
, Walbot
V.
, Davis
R. W.
(1992
) Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C 1.
Science,
258
, 1773
–1775
Ludwig
S. R.
, Habera
L. F.
, Dellaporta
S. L.
, Wessler
S. R.
(1989
) Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcriptional activators and contains the myc-homology region.
Proc. Natl. Acad. Sci. USA
86
, 7092
–7096
Martin
C.
, Carpenter
R.
, Sommer
H.
, Saedler
H.
, Coen
E. S.
(1985
) Molecular analysis of instability in flower pigmentation of Antirrhinum majus following isolation of the pallida locus by transposon tagging.
EMBO J
4
, 1625
–1630
Martin
C.
, Prescott
A.
, Lister
C.
, Mackay
S.
(1989
) Activity of the transposon Tam3 in Antirrhinum and tobacco: possible role of DNA methylation.
EMBO J
18
, 997
–1004
Mattanovich
D.
, Ruker
F.
, Machado
A.
, Laimer
M.
, Regner
F.
, Steinkellner
H.
, Himmler
G.
, Katinger
H.
(1989
) Efficient transformation of Agrobacterium spp. by electroporation.
Nucl. Acids Res
17
, 6747
–.Mooney
M.
, Desnos
T.
, Harrison
K.
, Jones
J.
, Carpenter
R.
, Coen
E.
(1995
) Altered regulation of tomato and tobacco pigmentation genes caused by the delila gene of Antirrhinum.
Plant J
7
, 333
–339
Noda
K.-I.
, Glover
B. J.
, Linstead
P.
, Martin
C.
(1994
) Flower colour intensity depends on specialized cell shape controlled by a Myb-related transcription factor.
Nature
369
, 661
–664
Oppenheimer
D. G.
, Herman
P. L.
, Sivakumaran
S.
, Esch
J.
, Marks
M. D.
(1991
) A MYB gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules.
Cell
67
, 483
–493
Ptashne
M.
(1988
) How eukaryotic transcriptional activators work.
Nature
335
, 683
–689
Quattrocchio
F.
, Wing
J. F.
, van der Woude
K.
, Mol
J. N. M.
, Koes
R.
(1998
) Analysis of bHLH and MYB domain proteins: species-specific regulatory differences are caused by divergent evolution of target anthocyanin genes.
Plant J
13
, 475
–488
Sachs
T.
(1996
) Genes, cellular interactions and cell lineages in the determination of plant trichome spacing.
BioEssays
18
, 443
–445
Silverstone
A. L.
, Mak
P. Y. A.
, Casamitjana-Martinez
E.
, Sun
T. P.
(1997
) The new RGA locus encodes a negative regulator of gibberellin response in Arabidopsis thaliana.
Genetics
146
, 1087
–1099
Simon
R.
, Carpenter
R.
, Doyle
S.
, Coen
E.
(1994
) Fimbriata controls flower development by mediating between meristem and organ identity genes.
Cell
78
, 99
–107
Smith
D. L.
, Watt
W. M.
(1986
) Distribution of lithocysts, trichomes, hydathodes and stomata in leaves of Pilea cadierei Gagnip. and Guill. (Urticaceae).
Ann. Bot
58
, 155
–166
Sylvester
A.W.
, Smith
L.
, Freeling
M.
(1996
) Acquisition of identity in the developing leaf.
Ann. Rev. Cell Dev. Biol
12
, 257
–304
Tamagnone
L.
, Merida
A.
, Parr
A.
, Mackay
S.
, Culianez-Macia
F. A.
, Roberts
K.
, Martin
C.
(1998
) The AmMYB308 and AmMYB330 transcription factors from Antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco.
Plant Cell,
10
, 135
–154
van Houwelingen
A.
, Souer
E.
, Spelt
K.
, Kloos
D.
, Mol
J.
, Koes
R.
(1998
) Analysis of flower pigmentation mutants generated by random transposon mutagenesis in Petunia hybrida.
Plant J
13
, 39
–50
Yang
M.
, Sack
F.
(1995
) The TOO MANY MOUTHS and 4LIPS mutations affect stomatal production in Arabidopsis.
Plant Cell
7
, 2227
–2239
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1998
