14-3-3 proteins have been shown to interact with Raf-1 and cause its activation when overexpressed. However, their precise role in Raf-1 activation is still enigmatic, as they are ubiquitously present in cells and found to associate with Raf-1 in vivo regardless of its activation state. We have analyzed the function of the Drosophila 14–3-3 gene leonardo (leo) in the Torso (Tor) receptor tyrosine kinase (RTK) pathway. In the syncytial blastoderm embryo, activation of Tor triggers the Ras/Raf/MEK pathway that controls the transcription of tailless (tll). We find that, in the absence of Tor, overexpression of leo is sufficient to activate tll expression. The effect of leo requires D-Raf and Ras1 activities but not KSR or DOS, two recently identified essential components of Drosophila RTK signaling pathways. Tor signaling is impaired in embryos derived from females lacking maternal expression of leo. We propose that binding to 14–3-3 by Raf is necessary but not sufficient for the activation of Raf and that overexpressed Drosophila 14–3-3 requires Ras1 to activate D-Raf.
The Drosophila 14–3-3 protein Leonardo enhances Torso signaling through D-Raf in a Ras 1-dependent manner
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W. Li, E.M. Skoulakis, R.L. Davis, N. Perrimon; The Drosophila 14–3-3 protein Leonardo enhances Torso signaling through D-Raf in a Ras 1-dependent manner. Development 15 October 1997; 124 (20): 4163–4171. doi: https://doi.org/10.1242/dev.124.20.4163
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