The klumpfuss (klu) transcription unit in Drosophila gives rise to two different transcripts of 4.5 and 4.9 kb, both of which encode a putative transcription factor with four zinc-finger motifs of the C2H2 class. Zinc-finger 2–4 are homologous to those of the proteins of the EGR transcription factor family. As in the case of the most divergent member of the family, the Wilms' tumor suppressor gene (WT-1), klu contains an additional zinc finger, which is only distantly related. Loss of klumpfuss function is semilethal and causes a variety of defects in bristles and legs of adults, as well as in mouth hooks and brains of larvae. Analysis of the mutants indicates that klumpfuss is required for proper specification and differentiation of a variety of cells, including the sensory organ mother cells and those of the distal parts of tarsal segments.

REFERENCES

Bellen
H. J.
,
Vaessin
H.
,
Bier
E.
,
Kolodkin
A.
,
D'Evelyn
D.
,
Kooyer
S.
,
Jan
Y. N.
(
1992
)
The Drosophila couch potato gene, an essential gene required for normal adult behavior.
Genetics
131
,
365
375
Bier
E.
,
Ackermann
L.
,
Barbel
S.
,
Jan
L.
,
Jan
Y. N.
(
1988
)
Identification and characterization of a neuron-specific nuclear antigen in Drosophila.
Science
240
,
913
916
Bier
E.
,
Vaessin
H.
,
Sherperd
S.
,
Lee
K.
,
MC Call
V.
,
Barbel
S.
,
Ackermann
L.
,
Carretto
R.
,
Uemura
T.
,
Grell
E.
,
Jan
L. Y.
,
Jan
Y. N.
(
1989
)
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.
Genes Dev
3
,
1273
1287
Bier
E.
,
Vaessin
H.
,
Younger-Sherperd
S.
,
Jan
L. Y.
,
Jan
Y. N.
(
1992
)
deadpan, an essential pan-neural gene in Drosophila, encodes a Helix-Loop-Helix protein similar to the hairy gene product.
Genes Dev
6
,
2137
2151
Blair
S.
,
Giangrande
A.
,
Skeath
J. B.
,
Palka
J.
(
1992
)
The development of normal and ectopic sensilla in the wings of hairy and Hairy wing mutants of Drosophila.
Mech. Dev
38
,
3
16
Boulianne
G. L.
,
de la Concha
A.
,
Campos-Ortega
J. A.
,
Jan
L. Y.
,
Jan
Y. N.
(
1991
)
The Drosohila neurogenic gene neuralized encodes a novel protein and is expressed in precursors of larval and adult neurons.
EMBO J
10
,
2975
2983
Brand
M.
,
Perrimon
N.
(
1993
)
Targeted expression as a means ofaltering cell fates and generating dominant phenotypes.
Development
118
,
401
415
Brand
M.
,
Jarman
A. P.
,
Jan
L. Y.
,
Jan
Y. N.
(
1993
)
asense is a Drosophila neural precursor gene and is capable of initiating sense organ formation.
Development
119
,
1
17
Brown
N. M.
,
Kafatos
F. C.
(
1988
)
Functional cDNA libraries from Drosophila embryos.
J. Molec. Biol
203
,
425
437
Call
K. M.
,
Glaser
T.
,
Ito
C.Y.
,
Buckler
A. J.
,
Pelletier
J.
,
Haber
D. A.
,
Rose
E. A.
,
Kral
A.
,
Yeger
H.
,
Lewis
W. H.
,
Jones
C.
,
Housemann
D. E.
(
1990
)
Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilm's tumor locus.
Cell
60
,
509
520
Cubas
P.
,
de Celis
J. F.
,
Campuzano
S.
,
Modolell
J.
(
1991
)
Proneural clusters of achaete-scute expression and generation of sensory organs in the Drosophila imaginal wing disc.
Genes Dev
5
,
996
1008
Dominguez
M.
,
Campuzano
S.
(
1993
)
The asense gene, a member of the Drosophila achaete-scute complex, is a proneural and neural differentiation gene.
EMBO J
12
,
2049
2060
Fristrom
D.
,
Fristrom
J. W.
(
1975
)
The mechanism of evagination of imaginal discs of Drosophila meanogaster. I. General considerations.
Dev. Biol
43
,
1
23
Frommer
G.
,
Vorbrueggen
G.
,
Pasca
G.
,
Jaeckle
H.
,
Volk
T.
(
1996
)
Epidermal egr-like finger protein of Drosophila participates in myotube guidance.
EMBO J
15
,
1642
1649
Garcia-Bellido
A.
(
1979
)
Genetic analysis of the achaete-scute system of Drosopila melanogaster.
Genetics
91
,
491
520
Gessler
M.
,
Poustka
A.
,
Cavenee
W.
,
Neye
R. L.
,
Orkin
S.
,
Bruns
G.A.P.
(
1990
)
Homozygous deletion in Wilm's tumors of a zinc-finger gene identified by chromosome jumping.
Nature
343
,
774
778
Ghysen
A.
,
Dambly-Chaudiere
C.
(
1989
)
Genesis of the Drosophila nervous system.
Trends in Genet
5
,
251
255
Ghysen
A.
,
Dambly-Chaudiere
C.
,
Jan
L.Y.
,
Jan
Y.N.
(
1993
)
Cell interactions and gene interactions in peripheral neurogenesis.
Genes Dev
7
,
723
733
Gonzalez
F.
,
Romani
S.
,
Cubas
P.
,
Modolell
J.
,
Campuzano
S.
(
1989
)
Molecular analysis of the asense gene, a member of the achaete-scute complex of Drosophila melanogaster, and its novel role in optic lobe development.
EMBO J
8
,
3553
3562
Haber
D.A.
,
Sohn
R. L.
,
Buckler
A. J.
,
Pelletier
J.
,
Call
K.
,
Housman
D. E.
(
1991
)
Alternative splicing and genomic structure of the Wilm's tumor gene, WT1.
Proc. Natn. Acad. Sci. USA
88
,
9618
9622
Han
K.
,
Manley
J. L.
(
1993
)
Transcriptional repression by the Drosophila even-skipped protein: definition of a minimal repression domain.
Genes Dev
7
,
491
503
Hinz
U.
,
Giebel
B.
,
Campos-Ortega
J. A.
(
1994
)
The basic Helix-Loop-Helix domain of Drosophila lethal of scute protein is sufficient for proneural function and activates neurogenic genes.
Cell
76
,
77
87
Hortsch
M.
,
Bieber
A. J.
,
Patel
N. H.
,
Goodman
C.
(
1990
)
Differential splicing generates a nervous system-specific form of Drosophila neuroglian.
Neuron
4
,
697
709
Huang
F.
,
Dambly-Chaudiere
C.
,
Ghysen
A.
(
1991
)
Emergence of sense organs in the wing disc of Drosophila.
Development
111
,
1087
1096
Jarman
A. P.
,
Grau
Y.
,
Jan
L. Y.
,
Jan
Y. N.
(
1993
)
atonal is a proneural gene that directs chordotonal organ formation in the Drosophila nervous system.
Cell
73
,
1307
1321
Klambt
C.
(
1993
)
The Drosophila gene pointed encodes two ETS-like proteins which are involved in the development of the midline glial cells.
Development
117
,
163
176
Larson
, et al. 
(
1995
)
Subnuclear localization of WT-1 in splicing or transcription factor domains is regulated by altrnative splicing.
Cell
81
,
391
401
Licht
J. D.
,
Grossel
M. J.
,
Figge
J.
,
Hansen
U. M.
(
1990
)
Drosophila Kruppel protein is a transcriptional repressor.
Nature
353
,
569
571
Madden
S. L.
,
Rauscher
F. J.
(
1993
)
Positive and negative regulation of transcription and cell growth mediated by the EGR family of zinc-finger gene products.
Ann. N. Y. Acad. Sci
684
,
75
84
Martinez
C.
,
Modolell
J.
,
Garrel
J.
(
1993
)
Regulation of the proneural gene achaete by HLH protein.
Mol. Cell. Biol
13
,
3514
3521
Masucci
J. D.
,
Miltenberger
R. J.
,
Hoffman
F. M.
(
1990
)
Patternspecific expression of the Drosophila decapentaplegic gene in imaginal discs is regulated by 3cis-regulatory elements.
Genes Dev
4
,
2011
2023
Mitchell
P. J.
,
Tijan
R.
(
1989
)
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
Science
245
,
371
378
Nardelli
J.
,
Gibson
T.
,
Charnay
P.
(
1992
)
Zinc finger-DNA recognition: analysis of base specificity by site-directed mutagenesis.
Nucleic Acid Res
20
,
4137
4144
Pavlevitch
N. P.
,
Pabo
C. O.
(
1991
).
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
Science
252
,
809
817
Pelletier
J.
,
Bruening
W.
,
Kashtan
C. E.
,
Mauer
S. M.
,
Manivel
J. C.
,
Striegel
J. E.
,
Houghton
D. C.
,
Junien
C.
,
Habib
R.
,
Fouser
L.
,
Fine.
R. N.
,
Silverman
J. L.
,
Haber
D. A.
,
Housman
D.
(
1991
)
Germline mutations in the Wilm's tumor suppressor gene are associated with abnormal urogenital development in Denys-Dash Syndrome.
Cell
67
,
437
447
Pirrotta
V.
,
Hadfield
C.
,
Pretorius
H. J.
(
1983
)
Microdissection and cloning of the white locus and the 3B1-3C2 region of the Drosophila X-chromosome.
EMBO J
2
,
927
934
Postlethwait
J. H.
,
Schneidermann
H. A.
(
1971
)
Pattern formation and determination in the antenna of the homeotic mutant Antennapedia of Drosophila melanogaster.
Dev. Biol
25
,
606
640
Robertson
H. M.
,
Preston
C. R.
,
Phillis
R. W.
,
Jhonson-Schlitz
D.
,
Benz
W. K.
,
Engels
W. R.
(
1988
)
A stable source of P-element transposase in Drosophila melanogaster.
Genetics
118
,
461
470
Rodriguez
J.
,
Hernandez
R.
,
Modolell
J.
,
Ruiz-Gomez
M.
(
1990
)
Competence to develop sensory organs is temporally and spatially regulated in Drosophila epidermal primordia.
EMBO J
11
,
3583
3592
Romani
S.
,
Campuzano
S.
,
Macagno
E. R.
,
Modolell
J.
(
1989
)
Expression of achaete and scute genes in Drosophila imaginal discs and their function in sensory organ development.
Genes Dev
3
,
997
1007
Schubiger
G.
(
1974
)
Acquisition of differentiation competence in the imaginal leg of Drosophila. Roux's Arch.
Dev. Biol
174
,
303
311
Skeath
J. B.
,
Carrol
S. B.
(
1991
)
Regulation of achaete-scute gene expression and sensory organ pattern formation in the Drosophila wing.
Genes Dev
5
,
984
995
Tautz
D.
,
Pfeifle
C.
(
1989
)
A non radioactive in situ hybridization method for localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
Chromosoma
98
,
81
85
Villares
R.
,
Cabrera
C. V.
(
1987
)
The achaete-scute gene complex of Drosophila melanogaster, conserved domains in a subset of genes required for neurogenesis and their homology to myc.
Cell
50
,
415
424
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