During Caenorhabditis elegans embryogenesis, specific cells in the P1 lineage rotate their duplicated centrosome pair onto the anterior-posterior axis; this rotation is correlated with and necessary for a differential inheritance of cytoplasmic determinants in the daughter cells. Centrosome pair rotation is sensitive to inhibitors of actin and microtubule polymerization and may require microtubule attachment to a specific cortical site. We show that actin and the barbed-end binding protein, capping protein, transiently accumulate at this cortical site, possibly by assembly onto persistent remnants of previous cell divisions. Based on these observations, we propose a model for the molecular basis of centrosome rotation that is consistent with the dependence of rotation on actin filaments and microtubules.

REFERENCES

Allen
V. A.
,
Kropf
D. L.
(
1992
)
Nuclear rotation and lineage specification in Pelvetia embryos.
Development
115
,
873
883
Barstead
R. J.
,
Waterston
R. H.
(
1991
)
Vinculin is essential for muscle function in the nematode.
J. Cell Biol
114
,
715
724
Bowerman
B.
,
Draper
B. W.
,
Mello
C. C.
,
Priess
J. R.
(
1993
)
The maternal gene skn-1 encodes a protein that is distributed unequally in early C. elegans embryos.
Cell
74
,
443
452
Brenner
S.
(
1974
)
The genetics of Caenorhabditis elegans.
Genetics
77
,
71
94
Caldwell
J. E.
,
Heiss
S. G.
,
Mermall
V.
,
Cooper
J. A.
(
1989
)
Effects of CapZ, an actin capping protein of muscle, on the polymerization of actin.
Biochemistry
28
,
8506
8514
Casella
J. F.
,
Craig
S. W.
,
Maack
D. J.
,
Brown
A. E.
(
1987
)
CapZ (32/32), a barbed end actin-capping protein, is a component of the Z-line of skeletal muscle.
J. Cell Biol
105
,
371
379
Cowan
A. E.
,
McIntosh
J. R.
(
1985
)
Mapping the distribution of differentiation potential for intestine, muscle and hypodermis during early development in Caenorhabditis elegans.
Cell
41
,
923
932
Dan
K.
,
Ito
S.
(
1984
)
Studies on unequal cleavages in molluscs: I. Nuclear behavior and anchorage of a spindle pole to cortex as revealed by isolation technique.
Dev. Growth and Differ
26
,
249
262
Eshel
D.
,
Urrestarazu
L. A.
,
Vissers
S.
,
Jauniaux
J.-C.
,
van Vliet-Reedijk
J. C.
,
Planta
R. J.
,
Gibbons
I. R.
(
1993
)
Cytoplasmic dynein is required for normal nuclear segregation in yeast.
Proc. Natl. Acad. Sci. USA
90
,
11172
11176
Gill
S. R.
,
Schroer
T. A.
,
Szilak
I.
,
Steuer
E. R.
,
Sheetz
M. P.
,
Cleveland
D. W.
(
1991
)
Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein.
J. Cell Biol
115
,
1639
1650
Hyman
A. A.
(
1989
)
Centrosome movement in the early divisions of Caenorhabditis elegans, a cortical site determining centrosome position.
J. Cell Biol
109
,
1185
1193
Hyman
A. A.
,
White
J. G.
(
1987
)
Determination of cell division axes in the early embryogenesis of Caenorhabditis elegans.
J. Cell Biol
105
,
2123
2135
Kemphues
K. J.
,
Priess
J. R.
,
Morton
D. G.
,
Cheng
N. S.
(
1988
)
Identification of genes required for cytoplasmic localization in early C. elegans embryos.
Cell
52
,
311
320
Krause
M.
,
Wild
M.
,
Rosenzweig
B.
,
Hirsh
D.
(
1989
)
Wild-type and mutant actin genes in Caenorhabditis elegans.
J. Mol. Biol
208
,
381
392
Laufer
J. S.
,
Bazzicalupo
P.
,
Wood
W. B.
(
1980
)
Segregation of developmental potential in early embryos of Caenorhabditis elegans.
Cell
19
,
569
577
Lees-Miller
J. P.
,
Helfman
D. M.
,
Schroer
T. A.
(
1992
)
A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility.
Nature
359
,
244
246
Lessard
J. L.
(
1988
)
Two Monoclonal Antibodies to Actin, One Muscle Selective and One Generally Reactive.
Cell Motil. Cytoskel
10
,
349
362
Lye
R.J.
,
Porter
M. E.
,
Scholey
J. M.
,
McIntosh
J. R.
(
1987
)
Identification of a microtubule-based cytoplasmic motor in the nematode C. elegans.
Cell
51
,
309
318
McDonald
H. B.
,
Stewart
R. J.
,
Goldstein
L. S.
(
1990
)
The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor.
Cell
63
,
1159
1165
McIntosh
J. R.
,
Porter
M. E.
(
1989
)
Enzymes for microtubule-dependent motility.
J. Biol. Chem
264
,
6001
6004
Mello
C. C.
,
Draper
B. W.
,
Krause
M.
,
Weintraub
H.
,
Priess
J. R.
(
1992
)
The pie-1 and mex-1 genes and maternal control of blastomere identity in early C. elegans embryos.
Cell
70
,
163
176
Palmer
R. E.
,
Sullivan
D. S.
,
Huffaker
T.
,
Koshland
D.
(
1992
)
Role of astral microtubules and actin in spindle orientation and migration in the budding yeast, Saccharomyces cerevisiae.
J. Cell Biol
119
,
583
593
Priess
J. R.
,
Thomson
J. N.
(
1987
)
Cellular interactions in early C. elegans embryos.
Cell
48
,
241
250
Priess
J. R.
,
Schnabel
H.
,
Schnabel
R.
(
1987
)
The glp-1 locus and cellular interactions in early C. elegans embryos.
Cell
51
,
601
611
Schierenberg
E.
(
1987
)
Reversal of cellular polarity and early cell-cell interaction in the embryos of Caenorhabditis elegans.
Dev. Biol
122
,
452
463
Schnabel
R.
(
1991
)
Cellular interactions involved in the determination of the early C. elegans embryo.
Mech. Dev
34
,
85
99
Schroer
T. A.
,
Sheetz
M. P.
(
1991
)
Two activators of microtubule-based vesicle transport.
J. Cell Biol
115
,
1309
1318
Strome
S.
,
Wood
W. B.
(
1983
)
Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos.
Cell
35
,
15
25
Strome
S.
(
1986
)
Fluorescence visualization of the distribution of microfilaments in gonads and early embryos of the nematode Caenorhabditis elegans.
J. Cell Biol
103
,
2241
2252
Strome
S.
(
1993
)
Determination of cleavage planes.
Cell
72
,
3
6
Sulston
J. E.
,
Schierenberg
E.
,
White
J. G.
,
Thomson
J. N.
(
1983
)
The embryonic cell lineage of the nematode Caenorhabditis elegans.
Dev. Biol
100
,
64
119
Waddle
J. A.
,
Cooper
J. A.
,
Waterston
R. H.
(
1993
)
Theand subunits of nematode actin capping protein function in yeast.
Mol. Biol. Cell
4
,
907
917
Walker
R. A.
,
Salmon
E. D.
,
Endow
S. A.
(
1990
)
The Drosophila claret segregation protein is a minus end directed motor molecule.
Nature
347
,
780
782
Wolf
N.
,
Priess
J.
,
Hirsh
D.
(
1983
)
Segregation of germline granules in early embryos of Caenorhabditis elegans, an electron microscopic analysis.
J. Embryol. Exp. Morphol
73
,
297
306
Yamashiki
N.
,
Kawamura
K.
(
1986
)
Microdissection studies on the polarity of unequal divisions in grasshopper neuroblasts. I. Subsequent divisions in neuroblast-type cells produced against the polarity by micromanipulation.
Exp. Cell. Res
166
,
127
138
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