Signaling between nerve and muscle is mediated by multiple mechanisms, including two transcriptional pathways. Signals provided by the nerve terminal activate transcription of acetylcholine receptor (AChR) genes in myofiber nuclei near the synaptic site, and signals associated with myofiber electrical activity inactivate AChR gene expression throughout the myofiber. These opposing effects of innervation are conferred by 1.8 kb of 5′ flanking DNA from the AChR δ subunit gene. These results raise the possibility that synapse-specific and electrical activity-dependent gene expression are mediated by the same DNA sequence and that activation and repression are determined by differential regulation of the same DNA binding protein. We produced transgenic mice carrying AChR δ subunit-hGH gene fusions, and we show here that a binding site (E-box) for myogenic basic helix-loop-helix proteins is required for electrical activity-dependent but not for synapse-specific gene expression of the δ subunit gene. These results indicate that a change in the expression or activity of an E-box binding protein(s) mediates electrical activity-dependent gene regulation and that synapse-specific and electrical activity-dependent gene expression require different DNA sequences. Moreover, we show here that the cis-acting elements for both aspects of innervation-dependent gene regulation are contained in 181 bp of 5′ flanking DNA from the AChR δ subunit gene.

Neuromuscular synapses form as a consequence of inductive interactions between nerve and muscle. Following contact between nerve and muscle during development, the nerve terminal and the postsynaptic region of the muscle fiber undergo a complex differentiation program and become specialized for synaptic transmission. One of the more striking and well-studied aspects of postsynaptic differentiation is the accumulation of acetylcholine receptors (AChRs) at synaptic sites, and the regulation of this process has served as a model for understanding mechanisms of synaptic differentiation (Jennings and Burden, 1993; Hall and Sanes, 1993).

Both post-translational and transcriptional mechanisms have an important role in accumulating AChRs at synaptic sites and excluding AChRs from non-synaptic regions of muscle. The post-translational process is controlled by agrin, a protein that is synthesized by motor neurons and deposited into the synaptic basal lamina, where it triggers a redistribution of AChRs from non-synaptic membrane to synaptic sites (McMahan, 1990; Nastuk and Fallon, 1993). The number and distribution of AChRs in skeletal muscle is also regulated by two transcriptional processes. One transcriptional pathway activates AChR gene expression in myofiber nuclei near the synaptic site, leading to an accumulation of AChR mRNA and protein at synaptic sites (Klarsfeld et al., 1991; Simon et al., 1992; Sanes et al., 1991; Burden, 1993). The signal for synapse-specific gene expression is not known, but like agrin, it is located in the synaptic basal lamina (Jo and Burden, 1992; Brenner et al., 1992; Goldman et al., 1991). Myofiber electrical activity controls a second transcriptional pathway that inactivates AChR gene expression in nuclei throughout the myofiber (Tsay and Schmidt, 1989; Merlie and Kornhauser, 1989; Simon et al., 1992). Because electrical activity regulates the expression of genes encoding adhesion molecules, transcription factors and voltage-gated channels (Covault et al., 1986; Hahn and Covault, 1992; Witzemann and Sakmann, 1991; Eftimie et al., 1991; Duclert et al., 1991; Neville et al., 1992; Yang et al., 1991), knowledge of the electrical activity-dependent signalling pathways will be an important step in understanding how myofiber electrical activity controls muscle physiology.

Cell culture studies have shown that the cis-acting sequences for electrical activity-dependent expression of the AChR δ subunit gene are contained in less than 200 bp of 5′ flanking DNA (Chahine et al., 1992; Dutton et al., 1993). This cis-acting region also confers muscle-specific gene expression and contains a binding site (E-box) for myogenic basic helix-loophelix (bHLH) proteins (MyoD, myogenin, myf-5, MRF-4). Because E-boxes in the AChR α and δ subunit genes are important for gene expression during muscle differentiation (Piette et al., 1990; Simon and Burden, 1993) and because myogenin expression increases following denervation (Witzemann and Sakmann, 1991; Eftimie et al., 1991; Duclert et al., 1991; Neville et al., 1992), myogenin could mediate the increase in AChR gene expresssion that follows loss of myofiber electrical activity.

In a previous study we used transgenic mice to show that the cis-acting sequences for both synapse-specific and electrical activity-dependent expression of the AChR δ subunit gene are contained in 1.8 kb of 5′ flanking DNA (Simon et al., 1992). These results raise the possibility that the same cis-acting element(s) confers both aspects of innervation-dependent expression. Here, we show that the critical cis-acting sequences for both aspects of innervation-dependent expression are not distributed throughout the δ subunit regulatory region but are contained in 181 bp of the δ subunit promoter. Moreover, we show that an E-box near the transcription start site of the δ subunit gene is required for electrical activity-dependent but not for synapse-specific gene expression. These results indicate that a change in the expression or activity of an E-box binding protein(s) mediates electrical activity-dependent gene regulation and that synapse-specific and electrical activity-dependent gene expression require different DNA sequences.

Founder mice carrying the δ( –1,823/mut. E1/+25)-hGH transgene were produced using methods similar to those described previously (Simon et al., 1992) and were the source of thirteen different lines. We prepared and stained extensor digitorum longus and soleus muscles for hGH expression as described previously (Simon et al., 1992). Synaptic hGH staining was observed in three lines, and the pattern of hGH staining in each line is indistinguishable from that in lines carrying an δ( – 1,823/+25)-hGH transgene. hGH expression is evident at all synaptic sites in muscle fibers from mice carrying a δ( – 1,823/+25)-hGH transgene (Simon et al., 1992) but only at 8-35% of synaptic sites in muscle fibers from mice carrying an E1-mutated transgene. We measured hGH mRNA in the three lines that have synaptic staining and in five of the ten lines that lack synaptic staining. Each of the three lines that have synaptic staining express hGH mRNA (Table 1), whereas four of the five lines that lacked synaptic staining do not express detectable hGH mRNA. One line lacks synaptic staining, but expresses very low levels of hGH mRNA.

Table 1
graphic
graphic

We produced six lines carrying the δ( –181/+25)-hGH transgene. Synaptic hGH staining was observed in three lines carrying the δ( –181/+25)-hGH transgene, and the pattern of hGH staining is indistinguishable from that in mice carrying a δ( – 1,823/+25)-hGH transgene. We measured hGH mRNA in the three lines that had synaptic staining and in one of the three lines that lacked synaptic staining; we found that only the lines with synaptic staining express detectable hGH mRNA. We produced one line carrying the CK(–1,200/+54)-hGH transgene (Dutton et al., 1993).

The sequence of E1 (CACCTG) was mutated (CACCAG) by site-directed mutagenesis, causing a >60-fold decrease in affinity for recombinant MyoD or myogenin (Simon and Burden, unpublished results) and a 20-fold reduction in δ subunit expression in cultured myotubes (Simon and Burden, 1993).

Leg muscles were denervated for 4-5 days, and mRNA levels were measured by RNase protection and quantitated by using a Phosphor-Imager as described previously (Simon et al., 1992).

AChR mRNA in adult myofibers is concentrated at synaptic sites (Merlie and Sanes, 1985; Fontaine et al., 1988; Goldman and Staple, 1989; Brenner et al., 1990). In a previous study we used transgenic mice to study the mechanisms responsible for localizing AChR mRNAs to synaptic sites (Simon et al., 1992). These mice carry a gene fusion between 1.8 kb of 5′ flanking DNA from the murine AChR δ subunit gene and the human growth hormone (hGH) gene. Because hGH is processed in intracellular organelles prior to secretion, and because these organelles are closely associated with nuclei, we could infer the nuclear source of hGH transcription by studying the spatial distribution of intracellular hGH by immunocytochemistry. We showed that hGH is restricted to the synaptic region of the myofiber, and we concluded that transcription of the endogenous AChR δ subunit gene is confined to nuclei situated at the synaptic site (Simon et al., 1992; see also Klarsfeld et al., 1991; Sanes et al., 1991; Duclert et al., 1993).

This 1.8 kb of 5′ flanking DNA contains three binding sites (E-boxes) for myogenic basic helix-loop-helix (bHLH) proteins. The E-box nearest to the transcription start site, E1, unlike the other two E-boxes, is important for muscle-specific gene expression (Simon and Burden, 1993). To determine whether E1 is necessary for synapse-specific gene expression, we produced mice carrying a transgene with a mutation in E1. Fig. 1 shows that this transgene, δ( – 1,823/mut. E1/+25)-hGH, is expressed selectively at synaptic sites. These results demonstrate that E1 is not required for synapse-specific expression.

Fig. 1.

hGH expression is restricted to synaptic sites in muscle fibers from mice carrying either an δ( – 1,823/+25)-hGH (A,B) or an δ( – 1,823/mut. E1/+25)-hGH transgene (C,D). Intracellular hGH is contained within organelles associated with secretion, including the endoplasmic reticulum, the Golgi apparatus and secretory vesicles (Simon et al., 1992). Synaptic sites (A,C) were identified by staining with tetramethylrhodamine α-bungarotoxin (TMR-α-BGT), and hGH (B,D) was detected by indirect immunofluorescence (Simon et al., 1992). Bar, 10 μm.

Fig. 1.

hGH expression is restricted to synaptic sites in muscle fibers from mice carrying either an δ( – 1,823/+25)-hGH (A,B) or an δ( – 1,823/mut. E1/+25)-hGH transgene (C,D). Intracellular hGH is contained within organelles associated with secretion, including the endoplasmic reticulum, the Golgi apparatus and secretory vesicles (Simon et al., 1992). Synaptic sites (A,C) were identified by staining with tetramethylrhodamine α-bungarotoxin (TMR-α-BGT), and hGH (B,D) was detected by indirect immunofluorescence (Simon et al., 1992). Bar, 10 μm.

AChR gene expression increases following denervation, causing an increase in the abundance of AChR mRNAs (Jennings and Burden, 1993; Hall and Sanes, 1993). We showed that 1.8 kb of 5′ flanking DNA from the δ subunit gene confers electrical activity-dependent gene regulation, because hGH mRNA levels are 10- to 20-fold higher in denervated than in innervated muscle of mice carrying a δ( –1,823/+25)-hGH transgene (Simon et al., 1992; see also Merlie and Kornhauser, 1989). To determine whether E1 is required for such regulation, we denervated muscle from mice carrying the E1-mutated transgene and measured the level of hGH mRNA in innervated and denervated muscle. Fig. 2 shows that mutation of E1 blocks electrical activity-dependent regulation: muscle denervation causes a 27-fold increase in hGH expression in mice carrying the δ (–1,823/+25)-hGH transgene but only a 1.6-fold increase in mice carrying the E1-mutated transgene (Table 1). These results demonstrate that E1 is required for electrical activity-dependent gene regulation.

Fig. 2.

E1 is required for electrical activity-dependent gene regulation. The level of δ subunit mRNA is low in innervated muscle (I) and increases approx. 25-fold following denervation (D). hGH expression is 25- to 50-fold greater in denervated than in innervated muscle from mice carrying δ (–1,823/+25)-hGHorδ( –181/ +25)-hGH transgenes (Table 1). In contrast, hGH expression is similar in denervated and innervated muscle from mice carrying a δ (–1,823/mut. E1/ +25)-hGH transgene. The abundance of actin mRNA (AC) is approx. 20 % lower in denervated than in innervated muscle (Simon et al., 1992). The position of the protected RNA probe is indicated by an arrowhead

Fig. 2.

E1 is required for electrical activity-dependent gene regulation. The level of δ subunit mRNA is low in innervated muscle (I) and increases approx. 25-fold following denervation (D). hGH expression is 25- to 50-fold greater in denervated than in innervated muscle from mice carrying δ (–1,823/+25)-hGHorδ( –181/ +25)-hGH transgenes (Table 1). In contrast, hGH expression is similar in denervated and innervated muscle from mice carrying a δ (–1,823/mut. E1/ +25)-hGH transgene. The abundance of actin mRNA (AC) is approx. 20 % lower in denervated than in innervated muscle (Simon et al., 1992). The position of the protected RNA probe is indicated by an arrowhead

To delineate more precisely the cis-acting sequences for innervation-dependent gene expression, we constructed mice carrying a transgene containing only 181 bp of 5′ flanking DNA from the δ subunit gene. This transgene contains E1 but lacks E2 and E3. This regulatory region confers electrical activity-dependent expression in spontaneously active muscle cells in cell culture (Dutton et al., 1993), and Fig. 2 shows that this sequence confers electrical activity-dependent regulation in transgenic mice. Fig. 3 shows that the δ( –181/+25)-hGH transgene, like the δ(–1,823/+25)-hGH transgene, is selectively expressed at synaptic sites. Thus, the cis-acting sequences for both aspects of innervation-dependent expression are located near the proximal promoter.

Fig. 3.

181 bp of 5′ flanking DNA from the δ subunit gene is sufficient to confer synapse-specific expression. hGH expression is restricted to synaptic sites in muscle fibers from mice carrying a δ( –181/+25)-hGH transgene (A,B). hGH expression is found throughout the myofiber in mice carrying a creatine kinase (CK)hGH transgene (C,D). Synaptic sites (A,C) were identified by staining with TMR-α-BGT, and hGH (B,D) was detected by indirect immunofluorescence. hGH staining at synaptic sites is indicated by an arrow (B,D). Bar, 30 μm for A,B and 50 μm for C,D.

Fig. 3.

181 bp of 5′ flanking DNA from the δ subunit gene is sufficient to confer synapse-specific expression. hGH expression is restricted to synaptic sites in muscle fibers from mice carrying a δ( –181/+25)-hGH transgene (A,B). hGH expression is found throughout the myofiber in mice carrying a creatine kinase (CK)hGH transgene (C,D). Synaptic sites (A,C) were identified by staining with TMR-α-BGT, and hGH (B,D) was detected by indirect immunofluorescence. hGH staining at synaptic sites is indicated by an arrow (B,D). Bar, 30 μm for A,B and 50 μm for C,D.

To control for potential differences in the secretory pathway at synaptic and non-synaptic regions of skeletal muscle that might influence accumulation of hGH, we constructed transgenic mice carrying a gene fusion between hGH and the creatine kinase (CK) regulatory region (Materials and Methods). The CK gene is expressed in skeletal muscle, but is not regulated by innervation (Dutton et al., 1993; Chahine et al., 1992). Fig. 3 shows that hGH in these transgenic mice is not concentrated at synaptic sites but is found throughout the myofiber. Thus, neither sequences in the hGH gene or mRNA nor potential differences in the secretory pathway in synaptic and non-synaptic regions can account for selective expression of hGH at synaptic sites. Therefore, accumulation of hGH in the synaptic region of myofibers containing δ-hGH transgenes is due to sequences in the δ subunit gene.

This study demonstrates that the E-box near the transcription start site of the AChR δ subunit gene is required to confer electrical activity-dependent regulation, and these results indicate that an increase in the expression and/or activity of an E-box binding protein(s) mediates the increase in δ subunit expression following denervation.

Because myogenin expression increases following denervation in all species tested (Witzemann and Sakmann, 1991; Eftimie et al., 1991; Duclert et al., 1991; Neville et al., 1992), myogenin is a likely candidate to mediate electrical activity-dependent expression of downstream genes. The steps and mechanisms that mediate regulation of the myogenin gene by electrical activity are not clear, but an E-box in the regulatory region of the myogenin gene is thought to be important for gene expression during myogenesis (Yee and Rigby, 1993). Thus, it is possible that an E-box in the myogenin gene is required for electrical activity-dependent regulation. The regulatory region of the myogenin gene, however, contains additional cis-acting elements, including a MEF-2 binding site, which are important for myogenin gene expression during development (Edmondson et al., 1992; Cheng et al., 1993) and which may have a role in regulating myogenin expression in innervated and denervated muscle.

Multiple mechanisms for modulating the activity of E-box binding proteins have been described (Lassar et al., 1989; Benezra et al., 1990; Bengal et al., 1992; Li et al., 1992a), including phosphorylation catalyzed by protein kinase C (PKC) (Li et al., 1992b). PKC is a potential negative regulator of myogenic bHLH proteins in innervated muscle, since electrical activity increases PKC activity (Huang et al., 1992), and this increased PKC activity is important for repressing AChR expression in electrically active muscle (Huang et al., 1992; Klarsfeld et al., 1989; Laufer and Changeux, 1989). A single threonine residue in the basic region of myogenin is responsible for PKC-mediated inactivation of myogenin activity, and this critical threonine residue is conserved in MyoD, myf-5 and MRF-4 (Li et al., 1992b). Thus, all of the myogenic bHLH proteins are potential substrates for PKC. The data presented here are consistent with the idea that a decrease in myogenin expression and/or a decrease in the activity of myogenic bHLH proteins is responsible for the decrease in AChR expression in electrically active muscle.

E-boxes are clearly not sufficient to confer electrical activity-dependent regulation, since many genes that are expressed in skeletal muscle and that contain E-boxes are not regulated by electrical activity. Moreover, two E-boxes are present in the E1-mutated δ subunit gene, but these E-boxes do not confer regulation by electrical activity. Although the mechanisms that distinguish these different E-boxes remain obscure, these results suggest that differences in the core sequence, context and/or position of E-boxes are likely to be important for conferring electrical activity-dependent regulation.

Although our immunocytochemical experiments show that the pattern of hGH expression is not altered by a mutation in E1, the level of expression from synaptic nuclei does appear to be reduced. Because we are unable to detect expression from non-synaptic nuclei in innervated muscle, we do not know whether mutation of E1 causes a similar reduction in expression from non-synaptic nuclei. Thus, we cannot exclude the possibility that E1 contributes to quantitative differences in expression of the δ subunit gene between synaptic and non-synaptic nuclei.

The E-box near the transcription start site of the δ subunit gene is important for muscle-specific and electrical activity-dependent expression. Synapse-specific expression, however, does not require E1. Likewise, E-boxes are dispensable for synaptic expression of the AChR ε subunit gene (Duclert et al., 1993). The cis-acting region for synapse-specific expression contains potential binding sites for several DNA-binding proteins, including AP2, CBF40 and myb (Simon and Burden, 1993), and these proteins, or still others could have a role in synapse-specific gene expression. Knowledge that the regulatory element(s) that confers synapse-specific expression is located within 181 bp of the δ subunit gene should simplify identification of the key cis-acting sequence and facilitate characterization of the proteins that control expression of the δ subunit gene in synaptic nuclei.

This study was supported by research grants from the NIH (NS27963), the Muscular Dystrophy Association and by an NICHD contract (NO1-HD-0-2911) in support of DNX, Inc. We would like to thank Xuejun Zhu and Dr Charles Jennings for their numerous insights and suggestions during the course of this work. We are particularly thankful for the technical assistance of Tony Hsu. We also thank Jim Yeadon, Xuejun Zhu, Stephen Dyer, Alex Simon and Dr R. Lehmann for their comments on the manuscript.

Benezra
,
R.
,
Davis
,
R. L.
,
Lockshorn
,
D.
,
Turner
,
D. L.
and
Weintraub
,
H.
(
1990
).
The protein Id: a negative regulator of helix-loop-helix DNA binding proteins
.
Cell
61
,
49
59
.
Bengal
,
E. L.
,
Ransone
,
L.
,
Scharfmann
,
R.
,
Dwarki
,
V. J.
,
Tapscott
,
S.
,
Weintraub
,
H.
and
Verma
,
I. M.
(
1992
).
Functional antagonism between C-Jun and MyoD proteins: a direct physical association
.
Cell
68
,
507
519
.
Brenner
,
H.-R.
,
Witzemann
,
V.
and
Sakmann
,
B.
(
1990
).
Imprinting of acetylcholine receptor messenger RNA accumulation in mammalian neuromuscular synapses
.
Nature
334
,
544
547
.
Brenner
,
H. R.
,
Herczeg
,
A.
and
Slater
,
C. R.
(
1992
).
Synapse-specific expression of acetylcholine receptor genes and their products at original synaptic sites in rat soleus muscle fibers regenerating in the absence of innervation
.
Development
116
,
41
53
.
Burden
,
S. J.
(
1993
).
Synapse-specific gene expression
.
Trends in Genetics
9
,
12
16
Chahine
,
K. G.
,
Walke
,
W. D.
and
Goldman
,
D. A.
(
1992
).
102 base pair sequence of the nicotinic acetylcholine receptor delta-subunit gene confers regulation by muscle electrical activity
.
Development
115
,
213
219
.
Cheng
,
T. C.
,
Wallace
,
M. C.
,
Merlie
,
J. P.
and
Olson
,
E.N.
(
1993
).
Separable regulatory elements governing myogenin transcription in mouse embryogenesis
.
Science
261
,
215
218
.
Covault
,
J.
,
Merlie
,
J. P.
,
Goridis
,
C.
and
Sanes
,
J. R.
(
1986
).
Molecular forms of N-CAM and its RNA in developing and denervated skeletal muscle
.
J. Cell Biol
.
102
,
731
739
.
Duclert
,
A.
,
Piette
,
J.
and
Changeux
,
J. P.
(
1991
).
Influence of innervation on myogenic factors and acetylcholine receptor α-subunit mRNAs
.
NeuroReport
2
,
25
28
.
Duclert
,
A.
,
Savatier
,
N.
and
Changeux
,
J. P.
(
1993
).
An 83-nucleotide promoter of the acetylcholine recptor ε-subunit gene confers preferential synaptic expression in mouse muscle
.
Proc. Nat. Acad. Sci. USA
90
,
30433047
.
Dutton
,
E. K.
,
Simon
,
A. M.
and
Burden
,
S. J.
(
1993
).
Electrical activity-dependent regulation of the acetylcholine receptor δ-subunit gene, MyoD, and myogenin in primary myotubes
.
Proc. Nat. Acad. Sci. USA
90
,
20402044
.
Edmondson
,
D. G.
,
Cheng
,
T.-C.
,
Cserjesi
,
P.
,
Chakraborty
,
T.
and
Olson
,
E. N.
(
1992
).
Analysis of the myogenin promoter reveals an indirect pathway for the positive autoregulation mediated by the muscle-specific enhancer factor MEF-2
.
Mol. Cell. Biol
.
12
,
3665
3677
.
Eftimie
,
R.
,
Brenner
,
H. R.
and
Buonanno
,
A.
(
1991
).
Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity
.
Proc. Nat. Acad. Sci. USA
88
,
1349
1353
.
Fontaine
,
B.
,
Sassoon
,
D.
,
Buckingham
,
M.
and
Changeux
,
J. P.
(
1988
).
Detection of the nicotinic acetylcholine receptor α-subnit mRNA by in situ hybridization at neuromuscular junctions of 15-day-old chick striated muscle
.
EMBO J
.
7
,
603
609
.
Goldman
,
D.
and
Staple
,
J.
(
1989
).
Spatial and temporal expression of acetylcholine receptor mRNAs in innervated and denervated rat soleus muscle
.
Neuron
3
,
219
228
.
Goldman
,
D.
,
Carlson
,
B. M.
and
Staple
,
J.
(
1991
).
Induction of adult-type nicotinic acetylcholine receptor gene expression in non-innervated regenerating muscle
.
Neuron
7
,
649
658
.
Hahn
,
C
and
Covault
,
J.
(
1992
).
Neural regulation of N-cadherin gene expression in developing and adult skeletal muscle
.
J. Neurosci
.
12
,
46774687
.
Hall
,
Z. W.
and
Sanes
,
J. R.
(
1993
).
Synaptic structure and development: the neuromuscular junction
.
Cell/Neuron 72/10 (Suppl.), 99-121
.
Huang
,
C.-F.
,
Tong
,
J.
and
Schmidt
,
J.
(
1992
).
Protein kinase C couples membrane excitation to acetylcholine receptor gene inactivation in chick skeletal muscle
.
Neuron
9
,
13
20
.
Jennings
,
C. G. B.
and
Burden
,
S. J.
(
1993
).
Development of the neuromuscular junction
.
Curr. Opin. Neurobiol
.
3
,
75
81
.
Jo
,
S. A.
and
Burden
,
S. J.
(
1992
).
Synaptic basal lamina contains a signal for synapse-specific transcription
.
Development
115
,
673
680
.
Klarsfeld
,
A.
,
Bessereau
,
J.-L.
,
Salmon
,
A.-M.
,
Triller
,
A.
,
Babinet
,
C.
and
Changeux
,
J.-P.
(
1991
).
An acetylcholine receptor α-subunit promoter conferring preferential synaptic expression in muscle of transgenic mice
.
EMBO J
.
10
,
625
632
.
Klarsfeld
,
A.
,
Laufer
,
R.
,
Fontaine
,
B.
,
Devillers-Thiery
,
A.
,
Dubreuil
,
C.
and
Changeux
,
J.-P.
(
1989
).
Regulation of muscle AChR α subunit gene expression by electrical activity: involvement of protein kinase C and Ca2+
.
Neuron
2
,
1229
1236
.
Lassar
,
A. B.
,
Thayer
,
M. J.
,
Overell
,
R. W.
and
Weintraub
,
H.
(
1989
).
Transformation by activated ras or fos prevents myogenesis by inhibiting expression of MyoD1
.
Cell
58
,
659
667
.
Laufer
,
R.
and
Changeux
,
J.-P.
(
1989
).
Activity-dependent regulation of gene expression in muscle and neuronal cells
.
Mol. Neurobiol
.
3
,
1
53
.
Li
,
L.
,
Heller-Harrison
,
R.
,
Czech
,
M.
and
Olson
,
E. N.
(
1992a
).
Cyclic AMP-dependent protein kinase inhibits the activity of myogenic helix-loophelix proteins
.
Mol. Cell. Biol
.
12
,
4478
4485
.
Li
,
L.
,
Zhou
,
J.
,
James
,
G.
,
Heller-Harrison
,
R.
,
Czech
,
M.
and
Olson
,
E. N.
(
1992b
).
FGF inactivates myogenic helix-loop-helix proteins through phosphorylation of a conserved protein kinase C site in their DNA-binding domains
.
Cell
71
,
1181
1194
.
McMahan
,
U. J.
(
1990
).
The agrin hypothesis
.
Cold Spring Harb. Symp. Quant. Biol
.
55
,
407
418
.
Merlie
,
J. P.
and
Kornhauser
,
J. M.
(
1989
).
Neural regulation of gene expression by an acetylcholine receptor promoter in muscle of transgenic mice
.
Neuron
2
,
1295
1300
.
Merlie
,
J. P.
and
Sanes
,
J. R.
(
1985
).
Concentration of acetylcholine receptor mRNA in synaptic regions of adult muscle fibers
.
Nature
317
,
66
68
.
Nastuk
,
M. A.
and
Fallon
,
J. R.
(
1993
).
Agrin and the molecular choreography of synapse formation
.
Trends Neurosci
.
16
,
72
76
.
Neville
,
C. M.
,
Schmidt
,
M.
and
Schmidt
,
J.
(
1992
).
Response of myogenic determination factors to cessation and resumption of electrical activity in skeletal muscle: a possible role for myogenin in denervation supersensitivity
.
Cell Mol. Neurobiol
.
12
,
511
527
.
Piette
,
J.
,
Bessereau
,
J.-L.
,
Huchet
,
M.
and
Changeux
,
J.-P.
(
1990
).
Two adjacent MyoD1-binding sites regulate expression of the acetylcholine receptor α-subunit gene
.
Nature
345
,
353
355
.
Sanes
,
J. R.
,
Johnson
,
Y. R.
,
Kotzbauer
,
P. T.
,
Mudd
,
J.
,
Hanley
,
T.
,
Martinou
,
J.-C.
and
Merlie
,
J. P.
(
1991
).
Selective expression of an acetylcholine receptor-lacZ transgene in synaptic nuclei of adult muscle fibers
.
Development
113
,
1181
1191
.
Simon
,
A. M.
,
Hoppe
,
P.
and
Burden
,
S. J.
(
1992
).
Spatial restriction of AChR gene expression to subsynaptic nuclei
.
Development
114
,
545
553
.
Simon
,
A. M.
and
Burden
,
S. J.
(
1993
).
An E-box mediates activation and repression of the AChR δ subunit gene during myogenesis
.
Mol. Cell. Biol
.
13
,
5133
5140
.
Tsay
,
H.-J.
and
Schmidt
,
J.
(
1989
).
Skeletal muscle denervation activates acetylcholine receptor genes
.
J. Cell Biol
.
108
,
1523
1526
.
Witzemann
,
V.
and
Sakmann
,
B.
(
1991
).
Differential regulation of MyoD and myogenin mRNA levels by nerve induced muscle activity
.
FEBS Lett
.
282
,
259
264
.
Yang
,
J. S.-J.
,
Sladky
,
J. T.
,
Kallen
,
R. G.
and
Barchi
,
R. L.
(
1991
).
TTX-sensitive and TTX-insensitive sodium channel mRNA transcripts are independently regulated in adult skeletal muscle after denervation
.
Neuron
7
,
421
427
.
Yee
,
S.-P.
and
Rigby
,
P. W. J.
(
1993
).
The regulation of myogenin gene expression during the embryonic development of the mouse
.
Genes Dev
.
7
,
1277
1289
.