We have used carbocyanine dyes (DiI and DiO) to generate fate maps for the epiblast layer of the chick embryo between stage X and the early primitive streak stage (stages 2–3). The overall distribution of presumptive cell types in these maps is similar to that described for other laboratory species (zebrafish, frog, mouse). Our maps also reveal certain patterns of movement for these presumptive areas. Most areas converge towards the midline and then move anteriorly along it. Interestingly, however, some presumptive tissue types do not take part in these predominant movements, but behave in a different way, even if enclosed within an area that does undergo medial convergence and anterior movement. The apparently independent behaviour of certain cell populations suggests that at least some presumptive cell types within the epiblast are already specified at preprimitive streak stages.
REFERENCES
Ballard
W. W.
(1973
) A new fate map for Salmo gairdneri.
J. Exp. Zool
184
, 49
–74
Ballard
W. W.
(1981
) Morphogenetic movements and fate maps of vertebrates.
Am. Zool
21
, 391
–399
Ballard
W. W.
(1982
) Morphogenetic movements and fate maps of the crypriniform teleost, Catostomus commersoni (Lacepede).
J. Exp. Zool
219
, 301
–321
Butler
E.
(1935
) The developmental capacity of regions of the unincubated chick blastoderm as tested in chorio-allantoic grafts.
J. Exp. Zool
70
, 387
–338
Canning
D. R.
, Stern
C. D.
(1988
) Changes in the expression of the carbohydrate epitope HNK-1 associated with mesoderm induction in the chick embryo.
Development
104
, 643
–655
Dalton
A. J.
(1935
) The potencies of portions of young chick blastderms as tested in chorio-allantoic grafts.
J. Exp. Zool
71
, 17
–51
Delarue
M.
, Boucaut
J. C.
(1992
) A fate map of superficial and deep circumblastoporal cells in the early gastula of Pleurodeles waltl.
Development
114
, 135
–146
Eyal-Giladi
H.
, Kochav
S.
(1976
) From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick.
Dev. Biol
49
, 321
–337
Gallera
J.
(1971
) Primary induction in birds.
Adv. Morphogen
9
, 149
–180
Gallera
J.
, Nicolet
G.
(1969
) Le pouvoir inducteur de l'endoblaste presomptif contenu dans la ligne primitive jeune de poulet.
J. Embryol. Exp. Morph
21
, 105
–118
Hamburger
V.
, Hamilton
H. L.
(1951
) A series of normal stages in the development of the chick embryo.
J. Morph
88
, 49
–92
Ho
R. K.
(1992
) Axis formation in the embryo of the zebrafish Brachydanio rerio.
Development
1992
, 65
–73
Hoadley
L.
(1926
) The in situ development of sectioned chick blastderms.
Arch. Biol
36
, 225
–309
Hoadley
L.
(1926
) Developmental potencies of parts of the early blastoderm of the chick. I. The first appearance of the eye.
J. Exp. Zool
43
, 151
–178
Hoadley
L.
(1926
) Developmental potencies of parts of the early blastoderm of the chick. II. The epidermis and the feather primordia.
J. Exp. Zool
43
, 179
–196
Hoadley
L.
(1927
) Concerning the organization of potential areas in the chick blastoderm.
J. Exp. Zool
48
, 459
–473
Honig
M. G.
, Hume
R. I.
(1989
) DiI and DiO: versatile fluorescent dyes for neuronal labeling and pathway tracing.
Trends Neurosci
12
, 333
–336
Hunt
T. E.
(1937
) The development of gut and its derivatives from the mesoderm of early chick blastoderms.
Anat. Rec
68
, 349
–370
Izpisúa-Belmonte
J. C.
, De Robertis
E. M.
, Storey
K. G.
, Stern
C. D.
(1993
) The homeobox gene goosecoid and the origin of organizer cells in the early chick blastderm.
Cell
74
, 645
–659
Keller
R.
, Shih
J.
, Domingo
C.
(1992
) The patterning and functioning of protrusive activity during convergence and extension of the Xenopus organiser.
Development
1992
, 81
–91
Kimmel
C. B.
, Warga
R. M.
(1988
) Tissue-specific cell lineages originate in the gastrula of the zebrafish.
Science
231
, 365
–368
Kimmel
C. B.
, Law
R. D.
(1985
) Cell lineage of zebrafish blastomeres I. Cleavage pattern and cytoplasmic bridges between cells.
Dev. Biol
108
, 78
–85
Kimmel
C. B.
, Law
R. D.
(1985
) Cell lineage of zebrafish blastomeres II. Formation of the yolk syncytial layer.
Dev. Biol
108
, 86
–93
Lawson
K. A.
, Meneses
J. J.
, Pedersen
R. A.
(1991
) Clonal analysis of epiblast fate during germ layer formation in the mouse embryo.
Development
113
, 891
–991
Loveless
W.
, Bellairs
R.
, Thorpe
S. J.
, Page
M.
, Feizi
T.
(1990
) Developmental patterning of the carbohydrate antigen FC10. 2 during early embryogenesis in the chick.
Development
108
, 97
–106
Malan
M.
(1953
) The elongation of the primitive streak and the localization of the presumptive chorda-mesoderm on the early chick blastderm studied by means of coloured marks with Nile blue sulphate.
Arch. Biol
64
, 149
–182
Murray
P. D. F.
, Selby
D.
(1930
) Chorio-allantoic grafts of entire and fragmented blastoderms of the chick.
J. Exp. Biol
7
, 404
–415
New
D. A. T.
(1955
) A new technique for the cultivation of the chick embryo in vitro.
J. Embryol. Exp. Morph
3
, 326
–331
Olivo
O. M.
(1928
) Über die fruhzeitig Determinierung der Herzanlage beim Huhnerembryo und der histologische und physiologische Differenzierung in vitro.
Anat. Anz
66
, 108
–118
Olivo
O. M.
(1928
) Precoce determination de l'ebauche du coeur dans l'embryon de poulet et sa differenciation histologique et physiologique in vitro.
C. R. Assoc. Anat
23
, 357
–374
Oppenheimer
J. M.
(1936
) Processes of localization in developing Fundulus.
J. Exp. Zool
73
, 405
–444
Page
M.
(1989
) Changing patterns of cytokeratins and vimentin in the earky chick embryo.
Development
105
, 97
–107
Pannett
C. A.
, Compton
A.
(1924
) The cultivation of tissues in saline embryonic juice.
Lancet
206
, 381
–384
Pasteels
J.
(1936
) Études sur la gastrulation des vertebres meroblastiques. I. Teleosteens.
Arch. Biol
47
, 205
–308
Pasteels
J.
(1937
) Études sur la gastrulation des Vertebres meroblastiques II. Reptiles. III. Oiseaux. IV. Conclusions generales.
Arch. Biol
48
, 105
–488
Pasteels
J.
(1940
) Un aperçu comparatif de la gastrulation chez les Chordes.
Biol. Rev
15
, 59
–106
Pasteels
J.
(1945
) On the formation of the primary entoderm of the duck (Anas domestica) and on the significance of the bilaminar embryo in birds.
Anat. Rec
93
, 5
–21
Patterson
J. T.
(1909
) Gastrulation in the pigeon's egg. A morphological and experimental study.
J. Morph
20
, 65
–124
Rawles
M. E.
(1940
) The pigment-forming potency of early chick blastoderm.
Proc. Natn. Acad. Sci. USA
26
, 86
–94
Rudnick
D.
(1932
) Thyroid-forming potencies of the early ckick blastoderm. I.
J. Exp. Zool
62
, 287
–317
Rudnick
D.
(1935
) Regional restriction of potencies in the chick during embryogenesis.
J. Exp. Zool
71
, 83
–99
Rudnick
D.
(1938
) Thyroid-forming potencies of the early chick blastoderm. II.
J. Exp. Zool
79
, 399
–427
Rudnick
D.
(1938
) Differentiation in culture of pieces of the early chick blastoderm. II. Short primitive streak stages.
J. Exp. Zool
89
, 399
–427
Rudnick
D.
(1944
) Early history and mechanics of the chick blastoderm.
A review. Quart. Rev. Biol
19
, 187
–212
Rudnick
D.
(1948
) Prospective areas and differentiation potencies in the chick blastoderm.
Ann. NY Acad. Sci
49
, 761
–772
Schoenwolf
G. C.
(1992
) Morphological and mapping studies of the paranodal and postnodal levels of the neural plate during chick neurulation.
Anat. Rec
233
, 281
–290
Selleck
M. A. J.
, Stern
C. D.
(1991
) Fate mapping and cell lineage analysis of Hensen's node in the chick embryo.
Development
112
, 615
–626
Serbedzija
G. N.
, Fraser
S. E.
, Bronner-Fraser
M.
(1990
) Pathways of neural crest cell migration in the mouse as revealed by vital dye labelling.
Development
108
, 605
–612
Shih
J.
, Keller
R.
(1992
) Cell motility driving mediolateral intercalation in explants of Xenopus laevis.
Development
116
, 901
–914
Shih
J.
, Keller
R.
(1992
) Patterns of cell motility in the organizer and dorsal mesoderm of Xenopus laevis.
Development
116
, 915
–930
Spratt
N. T.
(1940
) An in vitro analysis of the organization of the eye-forming area in the early chick blastderm.
J. Exp. Zool
85
, 171
–209
Spratt
N. T.
(1942
) Location of organ-specific regions and their relationship to the development of the primitive streak in the early chick blastoderm.
J. Exp. Zool
89
, 69
–101
Spratt
N. T.
(1946
) Formation of the primitive streak in the explanted chick blastoderm marked with carbon particles.
J. Exp. Zool
103
, 259
–304
Spratt
N. T.
(1947
) Localization of the prospective neural plate in the primitive streak blastoderm of the chick.
Anat. Rec
99
, 654
–.Stern
C. D.
(1990
) The marginal zone and its contribution to the hypoblast and primitive streak of the chick embryo.
Development
109
, 667
–682
Stern
C. D.
, Canning
D. R.
(1990
) Origin of cells giving rise to mesoderm and endoderm in chick embryo.
Nature
343
, 273
–275
Stern
C. D.
, Ireland
G. W.
(1981
) An integrated experimental study of endoderm formation in avian embryos.
Anat. Embryol
163
, 245
–263
Strehlow
D.
, Gilbert
W.
(1993
) A fate map for the first cleavages of the zebrafish.
Nature
361
, 451
–453
Thorpe
S. J.
, Bellairs
R.
, Feizi
T.
(1988
) Developmental patterning of carbohydrate antigens during early embryogenesis of the chick: expression of antigens of the poly-N-acetyllactosamine series.
Development
102
, 193
–210
Trinkaus
J. P.
, Fink
R. D.
(1992
) On the convergent cell movements of gastrulation in Fundulus.
J. Exp. Zool
261
, 40
–61
Vakaet
L.
(1960
) Quelques precisions sur la cinematique de la ligne primitive chez le poulet.
J. Embryol. Exp. Morph
8
, 321
–326
Vakaet
L.
(1970
) Cinephotomicrographic investigations of gastrulation in the chick blastoderm.
Arch. Biol. (Liege)
81
, 387
–426
Waddington
C. H.
(1935
) The development of isolated parts of the chick blastoderm.
J. Exp. Zool
71
, 273
–288
Warga
R. M.
, Kimmel
C. B.
(1990
) Cell movement during epiboly and gastrulation in zebrafish.
Development
108
, 569
–580
Wetts
R.
, Fraser
S.
(1989
) Slow intermixing of cells during Xenopus embryogenesis contributes to the consistency of the blastomere fate map.
Development
105
, 9
–15
Winkel
G. K.
, Pedersen
R. A.
(1988
) Fate of the inner cell mass in mouse embryos as studied by microinjection of lineage tracers.
Dev. Biol
127
, 143
–156
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1994
