We have constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.

REFERENCES

Ashburner
M.
(
1991
)
Drosophila genetic maps.
Drosophila Inf. Service
69
,
1
399
Baker
N. E.
,
Rubin
G. M.
(
1989
)
Effect on eye development of dominant mutations in Drosophila homologue of the EGF receptor.
Nature
340
,
150
153
Baker
N. E.
,
Rubin
M. G.
(
1992
)
Ellipse mutations in the Drosophila homologue of the EGF receptor affect pattern formation, cell division, and cell death in eye imaginal discs.
Dev. Biol
150
,
381
396
Chou
T.-B.
,
Perrimon
N.
(
1992
)
Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.
Genetics
131
,
643
653
Clifford
R. J.
,
Schupbach
T.
(
1989
)
Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGF receptor gene.
Genetics
123
,
771
787
Evan
G. I.
,
Lewis
G. K.
,
Ramsay
G.
,
Bishop
J. M.
(
1985
)
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Mol. Cell. Biol
5
,
3610
3616
Fortini
M. E.
,
Rubin
G. M.
(
1990
)
Analysis of cis -acting requirements of the Rh3 and Rh4 genes reveals a bipartite organization to rhodopsin promoters in Drosophila melanogaster.
Genes Dev
4
,
444
463
Garcia-Bellido
A.
,
Dapena
J.
(
1974
)
Induction, detection and characterization of cell differentiation mutants in Drosophila.
Mol. Gen. Genet
128
,
117
130
Garcia-Bellido
A.
,
Merriam
J. R.
(
1969
)
Cell lineage of the imaginal discs in Drosophila gynandromorphs.
J. Exp. Zool
170
,
61
75
Geyer
P. K.
,
Corces
V. G.
(
1987
)
Separate regulatory elements are responsible for the complex pattern of tissue-specific and developmental transcription of the yellow locus in Drosophila melanogaster.
Genes Dev
1
,
996
1004
Golic
K. G.
(
1991
)
Site-specific recombination between homologous chromosomes in Drosophila.
Science
252
,
958
961
Golic
K. G.
,
Lindquist
S.
(
1989
)
The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome.
Cell
59
,
499
509
Hopp
T. P.
,
Prickett
K. S.
,
Price
V. L.
,
Libby
R. T.
,
March
C. J.
,
Cerretti
D. P.
,
Urdal
D. L.
,
Conlon
P. J.
(
1988
)
A short polypeptide marker sequence useful for recombinant protein identification and purification.
Bio. Technol
6
,
1204
1210
Hotta
Y.
,
Benzer
S.
(
1972
)
Mapping of behaviour in Drosophila mosaics.
Nature
240
,
527
535
Jan
L. Y.
,
Jan
Y. N.
(
1982
)
Antibodies to horseradish peroxidase as specific neuronal markers in Drosophila and grasshopper embryos.
Proc. Natl. Acad. Sci. USA
79
,
2700
2704
Janning
W.
(
1972
)
Aldehyde-oxidase as a cell marker for internal organs in Drosophila melanogaster.
Naturwissenschaften
59
,
516
517
Kankel
D. R.
,
Hall
J. C.
(
1976
)
Fate mapping of nervous system and other internal tissues in genetic mosaics of Drosophila melanogaster.
Dev. Biol
48
,
1
24
Karess
R. E.
,
Rubin
G. M.
(
1984
)
Analysis of P transposable element functions in Drosophila.
Cell
38
,
135
146
Lawrence
P. A.
(
1981
)
A general cell marker for clonal analysis of Drosophila development.
J. Embryol. Exp. Morph
64
,
321
332
Lis
J. T.
,
Simon
J. A.
,
Sutton
C. A.
(
1983
)
New heat shock puffs and beta-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene.
Cell
35
,
403
410
McLeod
M.
,
Volkert
F.
,
Broach
J.
(
1984
)
Components of the site-specific recombination system encoded by the yeast 2-micro circle.
Cold Spring Harbor Symp. Quant. Biol
49
,
779
787
Morgan
T. H.
(
1914
)
Mosaics and gynandromorphs in Drosophila.
Proc. Soc. Exp. Biol. Med
11
,
171
172
Nusslein-Volhard
C.
,
Wieschaus
E.
(
1980
)
Mutations affecting segment number and polarity in Drosophila.
Nature
287
,
795
801
O'Hare
K.
,
Rubin
G. M.
(
1983
)
Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome.
Cell
34
,
25
35
O'Kane
C. J.
,
Gehring
W. J.
(
1987
)
Detection in situ of genomic regulatory elements in Drosophila.
Proc. Natl. Acad. Sci. USA
84
,
9123
9127
Patterson
J. T.
(
1929
)
The production of mutations in somatic cells of Drosophila melanogaster by means of X-rays.
J. Exp. Zool
53
,
327
372
Price
J. V.
,
Clifford
R. J.
,
Schupbach
T.
(
1989
)
The maternal ventralizing locus torpedo is allelic to faint little ball, an embryonic lethal, and encodes the Drosophila EGF receptor homolog.
Cell
56
,
1085
1092
Ready
D. F.
(
1989
)
A multifaceted approach to neural development.
Trends Neurosci
12
,
102
110
Ready
D. F.
,
Hanson
T. E.
,
Benzer
S.
(
1976
)
Development of the Drosophila retina, a neurocrystalline lattice.
Dev. Biol
53
,
217
240
Rebay
I.
,
Fleming
R. J.
,
Fehon
R. G.
,
Cherbas
L.
,
Cherbas
P.
,
Artavanis-Tsakonas
S.
(
1991
)
Specific EGF repeats of Notch mediate interactions with Delta and Serrate: Implications for Notch as multifunctional receptor.
Cell
67
,
687
699
Rio
D. C.
,
Laski
F. A.
,
Rubin
G. M.
(
1986
)
Identification and immunochemical analysis of biologically active Drosophila P element transposase.
Cell
44
,
21
32
Rubin
G. M.
(
1985
)
P transposable elements and their use as genetic tools in Drosophila.
Trends Neurosci
6
,
231
233
Rubin
G. M.
,
Spradling
A. C.
(
1982
)
Genetic transformation of Drosophila with transposable element vectors.
Science
218
,
348
353
Schejter
E. D.
,
Shilo
B.-Z.
(
1989
)
The Drosophila EGF receptor homolog (DER) gene is allelic to faint little ball, a locus essential for embryonic development.
Cell
56
,
1093
1104
Shilo
B.-Z.
,
Raz
E.
(
1991
)
Developmental control by the Drosophila EGF receptor homolog DER.
Trends Genet
7
,
388
392
Spradling
A. C.
,
Rubin
G. M.
(
1983
)
The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene.
Cell
34
,
47
57
Steller
H.
,
Pirrotta
V.
(
1985
)
A transposable P vector that confers selectable G418 resistance to Drosophila larvae.
EMBO J
4
,
167
171
Stern
C.
(
1936
)
Somatic crossing over and segregation in Drosophila melanogaster.
Genetics
21
,
625
730
Thummel
C. S.
,
Pirrotta
V.
(
1992
)
New pCaSpeR P element Vectors.
Drosophila Inf. Service
71
,
150
–.
Tomlinson
A.
(
1985
)
The cellular dynamics of pattern formation in the eye of Drosophila.
J. Embryol. Exp. Morph
89
,
313
331
Wharton
K. A.
,
Johansen
K. M.
,
Xu
T.
,
Artavanis-Tsakonas
S.
(
1985
)
Nucleotide sequence from the neurogenic locus notch implies aT. Xu and G. M. Rubin1237Mosaic analysis in Drosophilagene product that shares homology with proteins containing EGF-like repeats.
Cell
43
,
567
581
Wolff
T.
,
Ready
D. F.
(
1991
)
The beginning of pattern formation in the Drosophila compound eye: The morphogenetic furrow and the second mitotic wave.
Development
113
,
841
850
Xu
T.
,
Artavanis-Tsakonas
S.
(
1990
)
deltex, a locus interacting with the neurogenic genes, Notch, Delta and mastermind in Drosophila melanogaster.
Genetics
126
,
665
677
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