The methylation status of a mouse metallothionein-I/human transthyretin fusion gene was studied during gametogenesis in transgenic mice. In the adult tissues of this mouse line, the promoter region of the transgene on chromosome 11 is methylated when it is maternally inherited and undermethylated when it is paternally inherited. Germ cells from various developmental stages of gametogenesis were isolated, and their DNAs were assayed using methylation-sensitive restriction endonucleases and the polymerase chain reaction. Only low to nonexistent levels of transgene methylation were detected in germ cells from 14.5-day-old male and female fetuses irrespective of the parental origin of the transgene. This undermethylated state persisted in oocytes from newborn females as well as in testicular spermatogenic cells and sperm. By contrast, the transgene promoter was completely methylated in fully grown oocytes arrested at the first meiotic prophase. The endogenous metallothionein-I gene promoter, located on a different chromosome, remained undermethylated at all stages examined, consistent with previous findings reported for a typical CpG island. Taken together, the results suggest that parental-specific adult patterns of transgene methylation are established during gametogenesis.
Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis
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T. Ueda, K. Yamazaki, R. Suzuki, H. Fujimoto, H. Sasaki, Y. Sakaki, T. Higashinakagawa; Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis. Development 1 December 1992; 116 (4): 831–839. doi: https://doi.org/10.1242/dev.116.4.831
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