Liver and spleen hematopoietic cell suspensions from 20-day-old-fetal rats were fractionated on Percoll gradients. A granulocyte-rich splenic fraction inhibited CFUe production by cultures of a CFUe-enriched liver fraction, and by cultures of unfractionated liver and spleen hematopoietic cells. Conditioned medium from the spleen cell fraction contained an inhibitor of relative molecular mass, Mr, 25–35 × 10(3). The sensitivity of spleen cells to the inhibitor varied with the age of the fetus from which they were derived (20-day-old less than 18-and 19-day-old). No such age-dependence was found for liver cells. The inhibitor affects cycling CFUe, blocks the lethal effect of AraC, does not appear to be lineage-specific and its influence can be reversed by washing.
Erythroid colony formation by fetal rat liver and spleen cells in vitro: inhibition by a low relative molecular mass component of fetal spleen
M.D. Nagel, J. Nagel; Erythroid colony formation by fetal rat liver and spleen cells in vitro: inhibition by a low relative molecular mass component of fetal spleen. Development 1 January 1992; 114 (1): 213–219. doi: https://doi.org/10.1242/dev.114.1.213
Download citation file:
The Forest of Biologists
The Forest of Biologists is a biodiversity initiative created by The Company of Biologists, with support from the Woodland Trust. For every Research and Review article published in Development a native tree is planted in a UK forest. In addition to this we are protecting and restoring ancient woodland and are dedicating these trees to our peer reviewers. Visit our virtual forest to learn more.
Propose a new Workshop for 2025
Do you have an idea for a Workshop? We are now accepting proposals for our 2025 Biologists Workshops programme. As the scientific organiser, your involvement will be focused on the science. We'll take care of all the logistics. In 2025 we'll continue our efforts to diversify our Workshop programme and will be reserving one of our Workshops for an application from a Global South (GS) country to host an event overseas.
Development presents… Outstanding Paper Prize Finalists
On Wednesday 19 April, Development hosted a webinar celebrating the finalists for Development’s Inaugural Outstanding Paper Prize. The talks and Q&As were hosted by our Deputy Editor, Steve Wilson (UCL).
Development Journal Meeting 2023
The Abstract deadline for our 2023 Journal Meeting ‘Unconventional and Emerging Experimental Organisms in Cell and Developmental Biology’ is 23 June 2023. Find out more and apply here
Keeping up with the Node
Thirteen years on from its launch, the Node continues to be a place to share research stories, opinions, job adverts and event listings. Development have published a recent Editorial reviewing some of our most popular features, as well as introducing some new initiatives.