Mouse stromal cell lines (FLS lines), established from the livers of 13-day gestation mouse fetus, supported the proliferation and differentiation of the erythroid progenitor cells from mouse fetal livers and bone marrow in a semisolid medium in the presence of erythropoietin. A large erythroid colony of over 1000 benzidine-positive erythroid cells was developed from a single erythroid progenitor cell on the FLS cell layer after 4 days of culture. When in close contact with the layer, the erythroid progenitor cells divided rapidly with an average generation time of 9.6 h and mature erythroid cells, including enucleated erythrocytes, were produced. The present studies demonstrate that the microenvironment created by the stromal cells can support the rapid expansion of erythropoietic cell population in the fetal liver of mice.
Our Transformative Journals have exceeded their OA growth targets
Call for papers: Modelling Development In Vitro
Our next special issue will be on 'Modelling Development In Vitro’ and is due to be published in late 2022. The submission deadline is 28 February 2022. More details can be found here.
25th anniversary of the zebrafish special issue
To celebrate the 25th anniversary of our 1996 zebrafish special issue, we have compiled all the original articles and their related content into an online collection. You can also read about the significance of this special issue for the zebrafish field in this Spotlight article.
Development Journal Meeting 2022: From Stem Cells to Human Development
Following a virtual meeting in 2020, we are delighted to announce that the fifth iteration of our popular Journal Meeting will be held from 11-14 September 2022 at the historic Wotton House, Surrey. You can register your interest here. More details to follow shortly.
The Node Network
The Node Network is a global directory of developmental and stem cell biologists, designed to help you find speakers, referees, panel members and potential collaborators. Find out more about the Node Network.
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